Cellular Immunity and Flow Cytometry Subcore

About

The mission of the flow cytometry subcore is to support all phases of experimental studies using flow cytometry including experimental design, staining panel design, laboratory procedures, collection of samples on the instrument, analysis of data and interpretation of results.

The flow cytometry core provides support for experiments utilizing flow cytometry.  These can be simpler experiments using one to three colors or cutting-edge experiments using up to 18 colors.  Support can be provided for all phases of these studies, including experimental design, staining panel design, laboratory procedures, collection of samples on the instrument, analysis of data and interpretation of results.  Our aim is to serve as a resource to promote research in HIV and HIV-related diseases and we can provide multiple levels of support ranging from answering questions by email or phone, to providing individual training in laboratory procedures and also providing samples of specialized fluorochrome-conjugated antibody reagents.

The specialized cellular immunoassay aims to train individuals in state-of-the-art functional immunoassays. We can aid in assay design, optimization and validation of new assays as well as provide training for: T cell functional assays (measures of proliferation, cytotoxicity, cytokine secretion, viral suppression, etc) and NK functional assays (cytotoxicity, cytokine secretion, viral suppression, ADCVI).

We can also perform small or pilot experiments that may, for example, be necessary for a grant application.  Please feel free to contact us. 

People

Stephen De Rosa
Subcore Director
Email: sderosa at fredhutch.org
Telephone: 206-667-1681206-667-1681

Ana Gervassi
Subcore Director
Email: ana.gervassi at cidresearch.org
Telephone: 206-256-7198

Contact

Stephen De Rosa is available for consultation by email, phone or in person.  The laboratory is located at Fred Hutchinson Cancer Research Center at the 1100 Eastlake Building.