Co-infections, in particular tuberculosis (TB), are becoming increasingly recognized as confounders in the HIV epidemic. It is well known that increased prevalence of TB cases is due to immune deficiency caused by HIV. Thus, biological dissection of the interplay between these two pathogens and their subsequent deleterious consequences are important areas of study to enable appropriate interventions.
The new HIV co-morbidities sub-core adds a number of immunological assays mainly focused on Mycobacterium tuberculosis (Mtb) and innate immunity such as:
- Innate Immunity Expertise
- Functional studies of monocytes, macrophages, and dendritic cells including: stimulation with TLR/NLR ligands or Mtb and measurement of cytokine secretion, effector function (such as NO production), and phagocytosis.
- siRNA transfection of primary monocytes and monocytic cell lines
- RNA analysis, including microarray experiments
- Analysis and measurement of DC maturation and activation (flow cytometry)
- Signaling assays in reconstitution systems, often with luciferase expressing reporter genes.
b. M. tuberculosis
- Growth of Mtb and other mycobacteria (BCG, M.avium)
- Infection of monocytes and macrophages with Mtb and measurement of cellular responses including replication (with lux expressing mycobacteria)
- Mouse models of Mtb infection including assays measuring T cell mediated immunity.
Thomas Hawn, MD, PhD
750 Republic St., Box 358061
Seattle, WA 98195-8061
thawn at uw.edu