{"id":916,"date":"2023-07-21T21:30:41","date_gmt":"2023-07-22T04:30:41","guid":{"rendered":"https:\/\/depts.washington.edu\/cfar\/wordpress\/?page_id=916"},"modified":"2023-07-22T14:13:43","modified_gmt":"2023-07-22T21:13:43","slug":"humoral-immunity-subcore","status":"publish","type":"page","link":"https:\/\/depts.washington.edu\/cfar\/cores\/immunology-retrovirology-and-cure-core\/humoral-immunity-subcore\/","title":{"rendered":"Humoral Immunity Subcore"},"content":{"rendered":"<p>The Humoral Immunity subcore offers the following services: antibody and antigen testing, recombinant protein and antibody production, viral and cell line culture, and neutralization assays.<\/p>\n<div class=\"tab-tour  vertical-tour\" id=\"tabs-tour-container\">\n<div class=\"screen-reader-text\"><\/div>\n<div class=\"row\">\n<div class=\"col-3\">\n<ul class=\"nav flex-column nav-pills\" id=\"tab-tour\" role=\"tablist\" aria-orientation=\"vertical\">\n<li class=\"nav-item\" role=\"presentation\"><button role=\"tab\" class=\"nav-link active\" type=\"button\" id=\"title-tab-tour-1\" data-toggle=\"tab\" href=\"#tab-tour-1\"  aria-controls=\"tab-tour-1\" aria-selected=\"true\" >Services<\/button><\/li>\n<li class=\"nav-item\" role=\"presentation\"><button role=\"tab\" class=\"nav-link\" type=\"button\" id=\"title-tab-tour-2\" data-toggle=\"tab\" href=\"#tab-tour-2\"  aria-controls=\"tab-tour-2\" aria-selected=\"false\" tabindex=\"-1\">People<\/button><\/li>\n<\/ul>\n<\/div>\n<div class=\"col-9\">\n<div class=\"tab-content\" id=\"tab-content-tab-tour\">\n<div class=\"tab-pane fade show active\" id=\"tab-tour-1\" role=\"tabpanel\" aria-labelledby=\"title-tab-tour-1\" tabindex=\"0\">\n<div class=\"content_block\" id=\"custom_post_widget-979\">\n<div class=\"su-spoiler su-spoiler-style-default su-spoiler-icon-caret su-spoiler-closed\" data-scroll-offset=\"0\" data-anchor-in-url=\"no\">\n<div class=\"su-spoiler-title\" role=\"button\"><span class=\"su-spoiler-icon\"><\/span>Antibody-based Analyses for Antibodies or Antigens<\/div>\n<div class=\"su-spoiler-content su-u-clearfix su-u-trim\">\nThree antibody-based analyses. Below are protocols and further information on the analyses for which we can provide assistance.<\/p>\n<ul>\n<li>Western blot for Envelope proteins<\/li>\n<li>HIV-1 Envelope gp120 ELISA for endpoint titer and avidity<\/li>\n<li>BioLayer interferometry (Octet)*<\/li>\n<\/ul>\n<p>*For more information on how to access this service, please contact Noah Sather at noah.sather@seattlechildrens.org\n<\/p>\n<\/div>\n<\/div>\n<div class=\"su-spoiler su-spoiler-style-default su-spoiler-icon-caret su-spoiler-closed\" data-scroll-offset=\"0\" data-anchor-in-url=\"no\">\n<div class=\"su-spoiler-title\" role=\"button\"><span class=\"su-spoiler-icon\"><\/span>Recombinant Protein and Antibody Production and Purification<\/div>\n<div class=\"su-spoiler-content su-u-clearfix su-u-trim\">\nTwo types of assays are available for recombinant protein research:<\/p>\n<ul>\n<li>Expression and Purification of HIV-1 Envelope gp120 and gp140<\/li>\n<li>Large-scale purification of IgG from human or macaque plasma<\/li>\n<\/ul>\n<p>For more information on how to access this service, please contact cfarinfo@uw.edu\n<\/p>\n<\/div>\n<\/div>\n<div class=\"su-spoiler su-spoiler-style-default su-spoiler-icon-caret su-spoiler-closed\" data-scroll-offset=\"0\" data-anchor-in-url=\"no\">\n<div class=\"su-spoiler-title\" role=\"button\"><span class=\"su-spoiler-icon\"><\/span>Virus and Cell Line Culture and Neutralization Assays<\/div>\n<div class=\"su-spoiler-content su-u-clearfix su-u-trim\">\nFour types of assays. Below are protocols and further information on the analyses for which we can provide assistance.<\/p>\n<ul>\n<li>Isolation of PBMC from PALL filters for subsequent bulk viral infection<\/li>\n<li>Bulk Viral Infection of PHA-activated PBMC<\/li>\n<li>Pseudovirus production<\/li>\n<li>TZM-bl neutralization assay for HIV-1 single round competent viruses<\/li>\n<\/ul>\n<p>For more information on how to access this service, please contact Noah Sather at noah.sather at cidresearch.org.\n<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div class=\"tab-pane fade show\" id=\"tab-tour-2\" role=\"tabpanel\" aria-labelledby=\"title-tab-tour-2\" tabindex=\"0\">\nNoah Sather, PhD<br \/>\nHumoral Immunity Subcore Director<br \/>\nEmail: noah.sather at cidresearch.org\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>The Humoral Immunity subcore offers the following services: antibody and antigen testing, recombinant protein and antibody production, viral and cell line culture, and neutralization assays.<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":870,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"templates\/template-no-hero.php","meta":{"footnotes":""},"class_list":["post-916","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/depts.washington.edu\/cfar\/wp-json\/wp\/v2\/pages\/916","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/depts.washington.edu\/cfar\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/depts.washington.edu\/cfar\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/cfar\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/cfar\/wp-json\/wp\/v2\/comments?post=916"}],"version-history":[{"count":3,"href":"https:\/\/depts.washington.edu\/cfar\/wp-json\/wp\/v2\/pages\/916\/revisions"}],"predecessor-version":[{"id":981,"href":"https:\/\/depts.washington.edu\/cfar\/wp-json\/wp\/v2\/pages\/916\/revisions\/981"}],"up":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/cfar\/wp-json\/wp\/v2\/pages\/870"}],"wp:attachment":[{"href":"https:\/\/depts.washington.edu\/cfar\/wp-json\/wp\/v2\/media?parent=916"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}