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Separation of Polar and Non Polar Substances


Hexane is very flammable

Potassium permanganate and sodium dichromate are strong oxidizers

Iodine causes burns.

Chemicals and Solutions: 

Distilled water


I2 crystals

KMnO4 crystals

Sodium dichromate crystals


2-1L separatory funnels

2 Ringstands, 2 rings, and 2 clamps

2 Pennyhead stoppers

2 Spatulas


  • Put ~300 mL water into each sep. funnel.
  • Add ~200 mL n-hexane to each sep. funnel.
  • Add a few KMnO4 crystals to one sep. funnel and shake.

     (KMnO4 will dissolve in the aq. layer).

  • Add a few I2 crystals to the other sep. funnel and shake.

     (I2 will dissolve in the organic layer).

  • As an extra bonus, a few crystals of sodium dichromate can be added to the sep. funnel already containing iodine so  that Husky colors are made.

To schedule a demonstration, please send an e-mail to the demonstration lab.



Eric Camp

Lecture Demonstration Technician

Bagley Hall 171

(206) 543-1606


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