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Joshua C. Vaughan

Assistant Professor of ChemistryJoshua Vaughan

Adjunct Assistant Professor of Physiology & Biophysics

Ph.D. Massachusetts Institute of Technology, 2005

(Analytical, Biological, and Physical Chemistry)

(206) 543-4644


Vaughan group website


Research Interests


The Vaughan group develops new tools for high resolution fluorescence microscopy and uses these tools, along with established ones, to understand the molecular-scale organization of biological systems. On the tool development side, they are synthesizing new fluorescent probes, creating new methods for tissue expansion and clearing, and developing assays to understand the organization of the genome. On the application side, they partner with biologists and pathologists to apply imaging tools to the study of mouse neuronal circuitry, immune cell development, and pathology. Group members use diverse skill sets in chemistry, biology, and optics to tackle these highly interdisciplinary problems.


Representative Publications


Decarreau, J.; Wagenbach, M.; Lynch, E.; Halpern, A. R.; Vaughan, J.C.; Kollman, J.; Wordeman, L. A tetrameric kinesin, Kif25, suppresses pre-mitotic centrosome separation to establish proper spindle orientation. Nat. Cell Biol. 2017, 19, 384–390.


Chozinski, T. C.*; Halpern, A. R.*; Okawa, H.; Kim, H.-J.; Tremel, G. J.; Wong, R. O. L.; Vaughan, J. C. Expansion Microscopy with Conventional Antibodies and Fluorescent Proteins. Nat. Methods 2016, 13, 485–488. (Featured on journal cover)


Jia, S.; Vaughan, J. C.; Zhuang, X. Isotropic 3D Super-Resolution Imaging With a Self-Bending Point Spread Function. Nature Photon. 2014, 8, 302–306.


Vaughan, J. C.; Dempsey, G. T.; Sun, E.; Zhuang, X. Phosphine quenching of cyanine dyes as a versatile tool for fluorescence microscopy. J. Am. Chem. Soc. 2013, 135, 1197–1200.


Vaughan, J. C.; Jia, S.; Zhuang, X. Ultrabright Photoactivatable Fluorophores Created by Reductive Caging. Nat. Methods 2012, 9, 1181–1184.


More Publications


Awards & Activities

  • NIH Transformative Research Award, 2017-2022
  • 2008-2015 Burroughs Wellcome Career Award at the Scientific Interface
  • 2008-2009 NIH Ruth L. Kirschstein National Research Service Award Postdoctoral Fellowship

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