{"id":3297,"date":"2025-04-01T15:20:19","date_gmt":"2025-04-01T22:20:19","guid":{"rendered":"https:\/\/depts.washington.edu\/chemwp\/chemwpnmr\/?p=3297"},"modified":"2025-04-01T15:20:19","modified_gmt":"2025-04-01T22:20:19","slug":"important-note-sample-preparation-and-health-of-the-nmr-instruments","status":"publish","type":"post","link":"https:\/\/depts.washington.edu\/chemwp\/chemwpnmr\/2025\/04\/important-note-sample-preparation-and-health-of-the-nmr-instruments\/","title":{"rendered":"IMPORTANT NOTE :  SAMPLE PREPARATION AND HEALTH OF THE NMR INSTRUMENTS"},"content":{"rendered":"<p>Dear Users<\/p>\n<p>The NMR instruments in our facility are not only expensive but they are result of years of\u00a0 extensive R&amp;D and exquisite engineering.\u00a0\u00a0 They are robust enough to tolerate quite a bit of abuse.<\/p>\n<p>But, there are limits to how much these versatile and delicate instrumentation can take and still keep functioning.<\/p>\n<p>These days by automation, all the way from ATMA probes to sample queueing with robots and smart software, we have almost eliminated the case of &#8216;user neglect or ignorance&#8217;\u00a0 that can damage instrument hardware.<\/p>\n<p>But, there is one area that is still the sole responsibility of the userbase, <span style=\"text-decoration: underline;\">which the folks who run the facility cannot control i.e.\u00a0 your NMR sample preparation. \u00a0<\/span> There are limits that need to be respected with the following parameters, that are intertwined with your NMR samples. Please take a moment to reflect on the quetions in <em>italics below :<\/em><\/p>\n<ul>\n<li><strong>Sample Temperature limits :<\/strong> <em>will the magnet be harmed at the temperature and duration at which I collect data ?<\/em><\/li>\n<li><strong>Behavior of samples as a function of temperature :<\/strong> <em>will it freeze or boil at the temperatures I reach ?\u00a0 Will that lead to sample breakage ?<\/em><\/li>\n<li><strong>Sample Ionic Concentrations : <\/strong><em>is it going to lead to sample RF heating ? is\u00a0 it going to cause irreversible damage to the NMR probehead ?<\/em><\/li>\n<li><strong>Kind of solvents you use to dissolve your NMR samples :<\/strong> <em>will there be unexpected phase changes as a function of time or temperature ? What will that do to the integrity of the NMR sample and tube ?<\/em><\/li>\n<li><strong>Suitability of the experiment you are attempting <em>vis-a-vis<\/em> the nature of your NMR sample :<\/strong> <em>is it going to overload the RF system and potentially damage the instrument ? Am I using an optimized parameter set to avoid that ?<\/em><\/li>\n<li><strong>NMR sample tube considerations<\/strong> :\u00a0 <em>is it a thick (enough) walled tube, if I am attempting an observation under high pressure inert atmosphere to prevent oxidation or line broadening ?\u00a0 if I am trying to maximize concentration with a thin walled tube,\u00a0 is it prone to breakage ? can I think of alternatives like coaxial capillary insert tubes for the purpose ?<\/em><\/li>\n<\/ul>\n<p>There are always creative alternatives to achieving your final goal without causing lasting harm to the instruments.\u00a0 But, <strong>it is an important first step for you to think about these before you submit your sample.\u00a0 When in doubt, please reach out to one of us for guidance.\u00a0\u00a0<\/strong><\/p>\n<p>Thank you for your co-operation.<\/p>\n<h2>NMR FACILITY<\/h2>\n<p>4\/1\/2025<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Dear Users The NMR instruments in our facility are not only expensive but they are result of years of\u00a0 extensive R&amp;D and exquisite engineering.\u00a0\u00a0 They are robust enough to tolerate quite a bit of abuse. But, there are limits to how much these versatile and delicate instrumentation can take and still keep functioning. These days [&hellip;]<\/p>\n","protected":false},"author":19,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[10,20],"tags":[],"_links":{"self":[{"href":"https:\/\/depts.washington.edu\/chemwp\/chemwpnmr\/wp-json\/wp\/v2\/posts\/3297"}],"collection":[{"href":"https:\/\/depts.washington.edu\/chemwp\/chemwpnmr\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/depts.washington.edu\/chemwp\/chemwpnmr\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/chemwp\/chemwpnmr\/wp-json\/wp\/v2\/users\/19"}],"replies":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/chemwp\/chemwpnmr\/wp-json\/wp\/v2\/comments?post=3297"}],"version-history":[{"count":4,"href":"https:\/\/depts.washington.edu\/chemwp\/chemwpnmr\/wp-json\/wp\/v2\/posts\/3297\/revisions"}],"predecessor-version":[{"id":3301,"href":"https:\/\/depts.washington.edu\/chemwp\/chemwpnmr\/wp-json\/wp\/v2\/posts\/3297\/revisions\/3301"}],"wp:attachment":[{"href":"https:\/\/depts.washington.edu\/chemwp\/chemwpnmr\/wp-json\/wp\/v2\/media?parent=3297"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/depts.washington.edu\/chemwp\/chemwpnmr\/wp-json\/wp\/v2\/categories?post=3297"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/depts.washington.edu\/chemwp\/chemwpnmr\/wp-json\/wp\/v2\/tags?post=3297"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}