Title: METADATA FOR PRIMARY PRODUCTION DATA SETS FROM PUMP CASTS COLUMBIA RIVER LMER PROJECT Contact: Lawrence F. Small College of Oceanic and Atmospheric Sciences Oregon State University Corvallis, OR 97331-5503 USA email: smalll@ccmail.orst.edu Principle Investigators (Institution) and research area: Fredrick Prahl (Oregon St. Univ. Oceanic and Atmospheric Science) geochemistry; Lawrence Small (Oregon St. Univ. Oceanic and Atmospheric Science) phytoplankton Data Description: Estuarine turbidity maxima (ETM) are hypothesized to be sites of enhanced biological activity and chemical transformations. An active microbial loop and enhanced copepod grazing relative to surrounding areas may lead to rapid regeneration of nutrients capable of fueling primary production. Production and its associated biochemical parameters can be used to map the ETM and determine the riverine versus marine contributions to ETM particulate matter. Spatial and Temporal Resolution: Pump cast sampling, and associated CTD casts took place from a fixed vessel during one 28-35 day cruise per year in 1990, 1991, 1992, 1995, and 1996. In 1997 there were 2 week cruies in May, July, and October. Methodology: Pump casts were conducted immediately following a CTD cast and are, thus, cross-referenced by CTD data parameters. Water was sampled from surface and near-bottom depths and assayed for chlorophyll-a and phaeophytin-a by standard fluorometric techniques. Primary production was estimated by standard 14C methods. During these incubations, fluorescence was measured before and after treatemnt with DCMU to estimate photosynthetic potential of the cells. Particulate organic carbon and nitrogen were measured from filtered surface and bottom water samples, total suspended particulate matter was determined gravimetrically. See Small and Morgan (1994) for details and further references on methods. Variables: Code cast-specific code number (see below) Date date in MM/DD/YY format Time Time of day (Pacific Standard Time) Depth depth in meters Salinity salinity Fluor chlorophyll a (mg/m^3) determined by fluorometer (1992 only) AC3chla chlorophyll a (mg/m^3) determined by AC-3 (see below) Chla chlorophyll -a (ug / L), measured Phaeo phaeopigment (ug / L), measured 14CProd primary production (mg C / m^3 / h) DCMU DCMU index POC particulate organic carbon (mg / L) POC* particulate organic carbon (mg / L). Beginning in 1997, the particulate organic carbon samples were also analyzed in a second lab, on a second analyzer. PN particulate nitrogen (mg / L) PN* particulate nitrogen (mg / L). Beginning in 1997, the particulate organic nitrogen samples were also analyzed in a second lab, on a second analyzer. Explanation of Code: Format of code is: YYBSENN YY first two digits indicate year B letter(s) indicates boundary location or tidal series as follows: U=upstream (beyond salt wedge), D=downstream (below estuarine turbidity maximum), N=neap tide, S=spring tide, SN=spring-neap transition, E= ebb, F=flood, L=long time series, YB=Young's Bay, CB=Cathlamet Bay S number of sample series at that location/tidal stage E event number (sequence) within that series (an 'E' or 'F' may follow this number indicating and ebb or flood tide sampling NN two (or three) digits indicate CTD cast number that corresponds to the pump cast * an asterisk after the code indicates CTD data taken from the downcast immediately prior to the pump cast Other data notes: *** the AC-3 unit was used beginning in 1995; data sets from before then do not contain this column The voltage reading from the AC-3 is transformed into a chlorophyll a value using the factory calibration coefficients. The voltage signal is proportional to chlorophyll absorption after light passage through a series of filters contained within the AC-3 unit. *** DCMU index is calculated as: [F(DCMU) - F(INITIAL)] / F(DCMU), where F(DCMU) is the DCMU-enhanced fluorescence of the whole water sample and F(INITIAL) is the fluorescence before DCMU addition. File format: comma-delineated ASCII with header; maximum number of records = 912; number of data columns = 12 References: Small, LF and SR Morgan. 1994. Phytoplankton attributes of the turbidity maximum of the Columbia River Estuary, USA. In: KR Dyer and RJ Orth, eds., Changes in fluxes in estuaries: Implications from science to management (ECSA22/ERF, Plymouth, September 1992). pp 465-472. Olsen and Olsen, Fredensborg, Denmark. ---------------------------------------------------------------------------------------------------------------------------------