{"id":1002,"date":"2016-12-30T09:53:39","date_gmt":"2016-12-30T09:53:39","guid":{"rendered":"http:\/\/depts.washington.edu\/diabetes\/?page_id=1002"},"modified":"2026-01-02T10:11:16","modified_gmt":"2026-01-02T18:11:16","slug":"chargeback-rates","status":"publish","type":"page","link":"https:\/\/depts.washington.edu\/diabetes\/viral-vector-and-transgenic-mouse\/chargeback-rates\/","title":{"rendered":"Chargeback Rates"},"content":{"rendered":"
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\n\n\t

Chargeback Rates<\/h1>\n<\/div>\n
\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
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Service<\/strong><\/p>\n<\/td>\n

\n

Unit<\/strong><\/p>\n<\/td>\n

\n

Price*<\/strong><\/p>\n<\/td>\n<\/tr>\n

\n

VIRUS SERVICES<\/strong><\/p>\n<\/td>\n<\/tr>\n

\n

Construct for lentivirus\/MMLV\/AAV production<\/p>\n<\/td>\n

\n

per construct<\/p>\n<\/td>\n

\n

$817.40<\/p>\n<\/td>\n<\/tr>\n

\n

Construct for shRNA lentivirus<\/p>\n<\/td>\n

\n

Per construct<\/p>\n<\/td>\n

\n

$838.90<\/p>\n<\/td>\n<\/tr>\n

\n

Lentivirus production; (12\u00d715 cm plates)<\/p>\n<\/td>\n

\n

12 plates<\/p>\n<\/td>\n

\n

$3,566.00<\/p>\n<\/td>\n<\/tr>\n

\n

AAV production (per roller bottle; RB)<\/p>\n<\/td>\n

\n

3 \/ 6 \/ 12 RB per prep
*3 RB minimum<\/p>\n<\/td>\n

\n

$1,190.00\/1,050.00\/900.00<\/p>\n<\/td>\n<\/tr>\n

\n

AAV aliquot for serotype testing<\/p>\n<\/td>\n

\n

per aliquot<\/p>\n<\/td>\n

\n

$156.80<\/p>\n<\/td>\n<\/tr>\n

\n

CRISPR SERVICES CELL LINES<\/strong><\/p>\n<\/td>\n<\/tr>\n

\n

Consultation, design and testing of guide RNA<\/p>\n<\/td>\n

\n

Per target<\/p>\n<\/td>\n

\n

$822.90<\/p>\n<\/td>\n<\/tr>\n

\n

Generation of CRISPR-edited knockout line<\/p>\n<\/td>\n

\n

Per target<\/p>\n<\/td>\n

\n

$1,433.00<\/p>\n<\/td>\n<\/tr>\n

\n

Generation of CRISPR-edited point mutation line<\/p>\n<\/td>\n

\n

Per target<\/p>\n<\/td>\n

\n

$2,437.00<\/p>\n<\/td>\n<\/tr>\n

\n

Generation of CRISPR-edited knock-in line<\/p>\n<\/td>\n

\n

Per target<\/p>\n<\/td>\n

\n

$3,537.00<\/p>\n<\/td>\n<\/tr>\n

\n

CRISPR SERVICES (ADDITIONAL)<\/strong><\/p>\n<\/td>\n<\/tr>\n

\n

Synthetic guide RNA (gRNA) (approx. $300)<\/p>\n<\/td>\n

\n

Each<\/p>\n<\/td>\n

\n

$300.00<\/p>\n<\/td>\n<\/tr>\n

\n

Targeting gRNA and donor construct design<\/p>\n<\/td>\n

\n

Hour<\/p>\n<\/td>\n

\n

$51.28<\/p>\n<\/td>\n<\/tr>\n

\n

gRNA insert and repair construct synthesis<\/p>\n<\/td>\n

\n

Base<\/p>\n<\/td>\n

\n

$1.17<\/p>\n<\/td>\n<\/tr>\n

\n

Cloning gRNA insert into targeting vector<\/p>\n<\/td>\n

\n

Clone<\/p>\n<\/td>\n

\n

$521.50<\/p>\n<\/td>\n<\/tr>\n

\n

Plasmid propagation (for transfection)<\/p>\n<\/td>\n

\n

Sample<\/p>\n<\/td>\n

\n

$119.80<\/p>\n<\/td>\n<\/tr>\n

\n

MOUSE RELATED SERVICES<\/strong><\/p>\n<\/td>\n<\/tr>\n

\n

CRISPR construct production<\/p>\n<\/td>\n

\n

See below<\/p>\n<\/td>\n

\n

Contact Core Director<\/p>\n<\/td>\n<\/tr>\n

\n

Lentivirus or AAV production<\/p>\n<\/td>\n

\n

See Below<\/p>\n<\/td>\n

\n

See above<\/p>\n<\/td>\n<\/tr>\n

\n

Use of Transgenic Resources Program<\/p>\n<\/td>\n

\n

See Transgenic Resources Program (http:\/\/uwtransgenics.org)<\/p>\n<\/td>\n

\n

Variable<\/p>\n<\/td>\n<\/tr>\n

\n

MOLECULAR BIOLOGY SERVICES<\/strong><\/p>\n<\/td>\n<\/tr>\n

\n

Quantitative real time PCR with mRNA copy number determination<\/strong><\/p>\n<\/td>\n<\/tr>\n

\n

TaqMan Probe<\/p>\n<\/td>\n

\n

Each<\/p>\n<\/td>\n

\n

$200.00<\/p>\n<\/td>\n<\/tr>\n

\n

Probe and Primer Design<\/p>\n<\/td>\n

\n

Each<\/p>\n<\/td>\n

\n

$76.28<\/p>\n<\/td>\n<\/tr>\n

\n

Singleplex Reaction \u2013 from RNA\/DNA<\/p>\n<\/td>\n

\n

Well<\/p>\n<\/td>\n

\n

$3.75\/3.39<\/p>\n<\/td>\n<\/tr>\n

\n

Multiplex Reaction \u2013 from RNA\/DNA<\/p>\n<\/td>\n

\n

Well<\/p>\n<\/td>\n

\n

$5.21\/4.85<\/p>\n<\/td>\n<\/tr>\n

\n

SYBR Green \u2013 from RNA\/DNA<\/p>\n<\/td>\n

\n

Well<\/p>\n<\/td>\n

\n

$3.14\/2.93<\/p>\n<\/td>\n<\/tr>\n

\n

Reverse Transcription of RNA into cDNA<\/p>\n<\/td>\n

\n

Sample<\/p>\n<\/td>\n

\n

$4.25<\/p>\n<\/td>\n<\/tr>\n

\n

Genotyping using TaqMan probes<\/strong><\/p>\n<\/td>\n<\/tr>\n

\n

Genotyping DNA Isolation<\/p>\n<\/td>\n

\n

Sample<\/p>\n<\/td>\n

\n

$13.55<\/p>\n<\/td>\n<\/tr>\n

\n

25 \u221dl reaction<\/p>\n<\/td>\n

\n

Sample<\/p>\n<\/td>\n

\n

$3.08<\/p>\n<\/td>\n<\/tr>\n

\n

12.5 \u221dl reaction<\/p>\n<\/td>\n

\n

Sample<\/p>\n<\/td>\n

\n

$2.73<\/p>\n<\/td>\n<\/tr>\n

\n

Nucleic Acid Prep<\/strong><\/p>\n<\/td>\n<\/tr>\n

\n

Total RNA\/DNA Mini<\/p>\n<\/td>\n

\n

Sample<\/p>\n<\/td>\n

\n

$17.83<\/p>\n<\/td>\n<\/tr>\n

\n

Total RNA\/DNA Maxi<\/p>\n<\/td>\n

\n

Sample<\/p>\n<\/td>\n

\n

$104.90<\/p>\n<\/td>\n<\/tr>\n

\n

mRNA Mini<\/p>\n<\/td>\n

\n

Sample<\/p>\n<\/td>\n

\n

$56.22<\/p>\n<\/td>\n<\/tr>\n

\n

mRNA Maxi<\/p>\n<\/td>\n

\n

Sample<\/p>\n<\/td>\n

\n

$252.20<\/p>\n<\/td>\n<\/tr>\n

\n

Quantitation of RNA\/DNA by RiboGreen\/PicoGreen<\/p>\n<\/td>\n

\n

Well<\/p>\n<\/td>\n

\n

$1.54<\/p>\n<\/td>\n<\/tr>\n

\n

Northern\/Southern Blot<\/strong><\/p>\n<\/td>\n<\/tr>\n

\n

Preparation<\/p>\n<\/td>\n

\n

Blot<\/p>\n<\/td>\n

\n

$250.40<\/p>\n<\/td>\n<\/tr>\n

\n

Probing of Blot<\/p>\n<\/td>\n

\n

Probe<\/p>\n<\/td>\n

\n

$280.80<\/p>\n<\/td>\n<\/tr>\n

\n

Other Molecular Biology Services<\/strong><\/p>\n<\/td>\n<\/tr>\n

\n

Cloning of cDNA into vector<\/p>\n<\/td>\n

\n

Construct<\/p>\n<\/td>\n

\n

$817.40<\/p>\n<\/td>\n<\/tr>\n

\n

Bacterial Plasmid Transformation<\/p>\n<\/td>\n

\n

Sample<\/p>\n<\/td>\n

\n

$45.32<\/p>\n<\/td>\n<\/tr>\n

\n

Primer Design and Synthesis<\/p>\n<\/td>\n

\n

Base<\/p>\n<\/td>\n

\n

$1.35<\/p>\n<\/td>\n<\/tr>\n

\n

Whole Plasmid Sequencing<\/p>\n<\/td>\n

\n

Sample<\/p>\n<\/td>\n

\n

$18.65<\/p>\n<\/td>\n<\/tr>\n

\n

Sequencing<\/p>\n<\/td>\n

\n

Rxn<\/p>\n<\/td>\n

\n

$9.76<\/p>\n<\/td>\n<\/tr>\n

\n

OTHER SERVICES<\/strong><\/p>\n<\/td>\n<\/tr>\n

\n

Training<\/p>\n<\/td>\n

\n

Hour<\/p>\n<\/td>\n

\n

$51.28<\/p>\n<\/td>\n<\/tr>\n

\n

Consultation<\/p>\n<\/td>\n

\n

Hour<\/p>\n<\/td>\n

\n

$93.46<\/p>\n<\/td>\n<\/tr>\n

\n

*Invoices paid with non-UW funds will be charged an additional 8% F&A rate.<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Rates are subject to change<\/p>\n<\/div><\/div><\/div><\/div><\/div><\/section>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":0,"parent":63,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-sidebar.php","meta":{"_mi_skip_tracking":false,"footnotes":""},"class_list":["post-1002","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/depts.washington.edu\/diabetes\/wp-json\/wp\/v2\/pages\/1002","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/depts.washington.edu\/diabetes\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/depts.washington.edu\/diabetes\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/diabetes\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/diabetes\/wp-json\/wp\/v2\/comments?post=1002"}],"version-history":[{"count":28,"href":"https:\/\/depts.washington.edu\/diabetes\/wp-json\/wp\/v2\/pages\/1002\/revisions"}],"predecessor-version":[{"id":14341,"href":"https:\/\/depts.washington.edu\/diabetes\/wp-json\/wp\/v2\/pages\/1002\/revisions\/14341"}],"up":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/diabetes\/wp-json\/wp\/v2\/pages\/63"}],"wp:attachment":[{"href":"https:\/\/depts.washington.edu\/diabetes\/wp-json\/wp\/v2\/media?parent=1002"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}