{"id":481,"date":"2016-10-30T22:55:49","date_gmt":"2016-10-30T22:55:49","guid":{"rendered":"http:\/\/depts.washington.edu\/diabetes\/?page_id=481"},"modified":"2021-09-14T11:49:19","modified_gmt":"2021-09-14T18:49:19","slug":"services-facilities","status":"publish","type":"page","link":"https:\/\/depts.washington.edu\/diabetes\/cellular-and-molecular-imaging\/services-facilities\/","title":{"rendered":"Services and Facilities"},"content":{"rendered":"
<\/div>
\n

The Cellular and Molecular Imaging Core has two main locations: the\u00a0Cellular and Molecular Imaging Core at VA Puget Sound Health Care System and in the Histology and Imaging Core<\/a> at UW SLU. These two laboratories provide state-of-the art facilities to Diabetes Research Center Affiliates and enable the following services to be offered:<\/p>\n

Conventional Bright Field and Fluorescence Microscopy<\/h3>\n

Nikon NiE<\/a> (VA site) upright motorized microscope configured for bright field (including DIC) and 4-color fluorescence (LED; AURA II), large field of view monochrome and color cameras, and high NA dry and oil immersion objective lenses.<\/p>\n

Nikon Ti2<\/a> (VA site) inverted microscope with 5-color LED (AURA III), large field of view monochrome and color cameras, and high NA dry and oil immersion objective lenses.<\/p>\n

Nikon 90i (SLU and VA sites) microscope configured for bright field, DIC and multichannel fluorescence<\/p>\n

Digital Pathology<\/h3>\n

Hamamatsu NanoZoomer Digital Pathology Virtual Microscope<\/strong><\/a> (SLU site) scans whole slides and digitizes tissue sections. Images can be viewed over a network or the internet and imported into Visiopharm software for image analysis.<\/p>\n

Confocal Microscopy<\/h3>\n

Nikon A1R HD<\/strong> dual scanning confocal microscope (VA site), is equipped with a high speed resonance scanner (15 fps for 1024×1024 full frame scanning or 420fps in band scan mode) and high resolution Galvano scanner. The system comprises 4 laser lines (405nm, 488nm, 561nm, 640nm) and numerous high numerical aperture dry, oil and water immersion objective lenses.<\/p>\n

Super-Resolution Microscopy<\/h3>\n

Nikon N-SIM E<\/a> (VA site)wide-field system offers lateral resolution of 115 nm and an axial resolution of 269 nm. This system includes 3 laser lines (405nm, 488nm, 561nm) and is compatible with standard labels and dyes.<\/p>\n

Nikon N-STORM<\/a> (VA site) provides 20nm lateral resolution and 50nm axial resolution for imaging using synthetic photo-switchable dyes with a specific buffering protocol. Specific features include high speed acquisition (up to 500Hz), 4 laser lines (405nm, 488nm, 561nm, 640nm) and utilizes the best available 100X TIRF objective lens.<\/p>\n

Laser Capture Microdissection<\/h3>\n

Leica LMD7<\/a> includes high powered dissection laser, linear-encoded scanning stage (100 nm XY repeatability) and collection from up to 3 slides simultaneously. System is equipped for bright field and DAPI, GFP, CFP, TRITC and Cy5 fluorescence. A range of objectives (1.25 – 63X) and collection devices (including 96-well plates) provides substantial flexibility.<\/p>\n

Live Cell Imaging<\/h3>\n

DeltaVision Elite (SLU site) and Nikon Ti2\/High Content (VA site) systems are equipped for live-cell imaging as well as conventional imaging for multichannel fluorescence.<\/p>\n

Image Analysis<\/h3>\n

Visiopharm Imaging Software<\/strong><\/a>\u00a0with AI\/Deep learning provides powerful automated tissue segmentation and image analysis. Numerous apps are available or can be developed by core staff.<\/p>\n

Nikon NIS Elements<\/a> integrates image acquisition, visualization and analysis in one platform. Visualization and analysis of conventional, confocal (z-stack) and time lapse datasets including deconvolution are available. Several automated image analysis routines are available and can be customized for new projects.<\/p>\n

Tissue Processing and Embedding<\/h3>\n