Bleach
Doing a wash load with bleach requires one cup bleach [5.25% sodium hypochlorite] per wash load [16 gallons] or one part in 256.
TUBE # |
DIRECTIONS: |
TOTAL DILUTION |
1 |
1 part bleach & 9 parts seawater |
1 : 10 |
2 |
1 part #1 and 9 parts seawater |
1 : 100 |
3 |
1 part #2 and 9 parts seawater |
1 : 1,000 |
4 |
1 part #3 and 9 parts seawater |
1 : 10,000 |
5 |
1 part #4 and 9 parts seawater |
1 : 100,000 |
6 |
1 part #5 and 9 parts seawater |
1 : 1,000,000 |
7 |
1 part #6 and 9 parts seawater |
1 : 10,000,000 |
8 |
PURE SEAWATER |
CONTROL |
Set up test tubes with about a half an inch of each of the above solutions.
- Add one drop of concentrated eggs to each.
- Swirl and let sit for one minute.
- Add one drop of diluted sperm suspension and swirl briefly.
- Wait 5 minutes.
- Score eggs for fertilization. If time permits can also score for later development [Next period see if divided. Next day see if there are swimming blastula.]