{"id":742,"date":"2021-11-16T20:57:59","date_gmt":"2021-11-16T20:57:59","guid":{"rendered":"https:\/\/depts.washington.edu\/eph\/wordpress\/?page_id=742"},"modified":"2022-01-18T18:06:50","modified_gmt":"2022-01-19T02:06:50","slug":"modeling-cardiac-pathology-in-duchenne-muscular-dystrophy-in-ipsc-derived-engineered-heart-tissue","status":"publish","type":"page","link":"https:\/\/depts.washington.edu\/eph\/wordpress\/modeling-cardiac-pathology-in-duchenne-muscular-dystrophy-in-ipsc-derived-engineered-heart-tissue\/","title":{"rendered":"Modeling cardiac pathology in Duchenne muscular dystrophy in iPSC-derived engineered heart tissue"},"content":{"rendered":"\n<p>Investigators: S<a href=\"https:\/\/depts.washington.edu\/eph\/wordpress\/?page_id=300\" data-type=\"page\" data-id=\"300\">amantha Bremner<\/a>, <a rel=\"noreferrer noopener\" href=\"https:\/\/depts.washington.edu\/eph\/wordpress\/?page_id=167\" data-type=\"page\" data-id=\"167\" target=\"_blank\">David Mack<\/a>, <a href=\"https:\/\/iscrm.uw.edu\/faculty\/nathan-sniadecki\/\" target=\"_blank\" rel=\"noreferrer noopener\">Nathan Sniadecki<\/a><\/p>\n\n\n\n<p>Cardiac disease is the leading cause of death in Duchenne muscular dystrophy. We differentiate human induced pluripotent stem cells bearing DMD dystrophin mutations to create 3-dimensional cardiac tissue. These are mounted suspended between rigid and flexible posts, so that extent of deflection of the flexible post provides a measure of the force of muscle contraction, revealing DMD-associated defects in contractile force and beat rate.<\/p>\n\n\n\n<div class=\"wp-block-image is-style-default\"><figure class=\"aligncenter size-medium\"><img data-recalc-dims=\"1\" loading=\"lazy\" decoding=\"async\" width=\"300\" height=\"286\" src=\"https:\/\/i0.wp.com\/depts.washington.edu\/eph\/wordpress\/wp-content\/uploads\/2022\/01\/cartoon.png?resize=300%2C286&#038;ssl=1\" alt=\"\" class=\"wp-image-1000\" srcset=\"https:\/\/i0.wp.com\/depts.washington.edu\/eph\/wordpress\/wp-content\/uploads\/2022\/01\/cartoon.png?resize=300%2C286&amp;ssl=1 300w, https:\/\/i0.wp.com\/depts.washington.edu\/eph\/wordpress\/wp-content\/uploads\/2022\/01\/cartoon.png?resize=768%2C732&amp;ssl=1 768w, https:\/\/i0.wp.com\/depts.washington.edu\/eph\/wordpress\/wp-content\/uploads\/2022\/01\/cartoon.png?resize=500%2C477&amp;ssl=1 500w, https:\/\/i0.wp.com\/depts.washington.edu\/eph\/wordpress\/wp-content\/uploads\/2022\/01\/cartoon.png?resize=800%2C763&amp;ssl=1 800w, https:\/\/i0.wp.com\/depts.washington.edu\/eph\/wordpress\/wp-content\/uploads\/2022\/01\/cartoon.png?w=1008&amp;ssl=1 1008w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/figure><\/div>\n\n\n\n<figure class=\"wp-block-video\"><video height=\"640\" style=\"aspect-ratio: 640 \/ 640;\" width=\"640\" autoplay controls loop preload=\"auto\" src=\"https:\/\/depts.washington.edu\/eph\/wordpress\/wp-content\/uploads\/2022\/01\/beating-ips-cardios.mp4\"><\/video><figcaption><strong>Beating human cardiomyocytes differentiated from induced pluripotent stem cells<\/strong><\/figcaption><\/figure>\n\n\n\n<figure class=\"wp-block-video\"><video height=\"476\" style=\"aspect-ratio: 1280 \/ 476;\" width=\"1280\" autoplay controls loop src=\"https:\/\/depts.washington.edu\/eph\/wordpress\/wp-content\/uploads\/2022\/01\/Fluo4.mp4\"><\/video><figcaption><strong>Calcium transients in contracting post-mounted human 3D engineered heart tissue differentiated from induced pluripotent stem cells<\/strong><\/figcaption><\/figure>\n","protected":false},"excerpt":{"rendered":"<p>Investigators: Samantha Bremner, David Mack, Nathan Sniadecki Cardiac disease is the leading cause of death in Duchenne muscular dystrophy. We differentiate human induced pluripotent stem cells bearing DMD dystrophin mutations to create 3-dimensional cardiac tissue. These are mounted suspended between rigid and flexible posts, so that extent of deflection of the flexible post provides a [&hellip;]<\/p>\n","protected":false},"author":4,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"site-sidebar-layout":"default","site-content-layout":"default","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"class_list":["post-742","page","type-page","status-publish","hentry"],"jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/depts.washington.edu\/eph\/wordpress\/wp-json\/wp\/v2\/pages\/742","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/depts.washington.edu\/eph\/wordpress\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/depts.washington.edu\/eph\/wordpress\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/eph\/wordpress\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/eph\/wordpress\/wp-json\/wp\/v2\/comments?post=742"}],"version-history":[{"count":10,"href":"https:\/\/depts.washington.edu\/eph\/wordpress\/wp-json\/wp\/v2\/pages\/742\/revisions"}],"predecessor-version":[{"id":1019,"href":"https:\/\/depts.washington.edu\/eph\/wordpress\/wp-json\/wp\/v2\/pages\/742\/revisions\/1019"}],"wp:attachment":[{"href":"https:\/\/depts.washington.edu\/eph\/wordpress\/wp-json\/wp\/v2\/media?parent=742"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}