The Cell Analysis Facility


The Cell Analysis Facility is located in the Department of Immunology at the UW Medicine South Lake Union Campus Room E-386.

The facility provides a broad range of instrumentation and expertise for addressing experimental issues related to single cells. The Facility makes available state-of-the-art instrumentation and techniques while developing new ways of answering questions about the nature and function of cells. Not only is instrumentation available to the University community, but it is intended that the Facility contribute to all aspects of the research process. This includes consultation in experimental design, technical assistance in the operation of the instruments, distribution of raw data files, archival data storage, offline data analysis workstations, interpretation of results, and the provision of facilities for the preparation and production of presentation graphics.

The facility makes collected data easily accesible via shared folders on the flowlab Google Drive that are linked to each uses own UW email address. User can login to their own UW google drive and navigare to the "Shared with me" link to access the flowlab folder with their data organized by instrument. The facility also maintains an additional back up copy of all data on a local hardrive system. Workstations for data analysis are available in the computer lab free of charge.

For instruction, introduction to flow cytometry courses are offered periodically, and individual instruction can be provided as needed. Please call us at 543-7006 for details.

For details of availability, time, and charges, please see the section on Frequently Asked Questions

Remember to acknowledge the valuable services provided by the Cell Analysis Facility in your research papers and publications: "This research was supported by the Cell Analysis Facility Flow Cytometry Shared Resource Lab in the Department of Immunology at the University of Washington."

CAF COVID-19 Policies

Data Analysis Computer Lab: The workstation in the Cell Analysis Facility are closer together than the recommended 6-foot social distancing outlined by the CDC. We are limiting the number of people that are in that space at any given time. Please give others sufficient space, we are limiting the computer room to two people at a time choose a workstation furthest away from others. Please sign up for the computer lab on the scheduling calendar to make sure that it is availble for you when you need it!
To analyze data from your lab or home, a FlowJo license can be purchased through the facility for more information, click here or contact the lab for more information.
Instrumentation Use: The facility is open to all uses however, staff is limited so please check with us if you require assistance to make sure that we will be available to help you.  
Day of scheduling: Instruments that have been scheduling in advance will be setup by the facility staff. If you sign up on the same day and no one else has already scheduled time on that instrument the instrument will not be turned on by the staff. You may email flowlab@uw.edu or call the lab (206) 543-7006 to request it be turned on and QC’d but be aware if you sign up late in the day or right before you want to use the instrument it may not be ready for you.

Please limit the number of people at the instruments there should only be one person at an instrument at a time unless you are involved in training. Do not come in groups to run samples, designate a single person to enter the facility. Please review the facility map Here


All users who run should be familiar with how to properly clean the instruments. You may need to shutdown the instruments if you are the last user scheduled. Directions are avialable on the this website in the Protocols menu under Instrument Guides.


Cancellatios: If you have scheduled in advance and need to cancel day of, make sure to email flowlab@uw.edu 1st thing in the morning so that we can remove you from the schedule.


If you have a cough, sore throat, chills or fever STAY HOME.
Please follow these procedures when entering the Facility.


Before entering the lab, please have mask or face covering on.
Cytometer Usage:

  1. Don new gloves before using a cytometer (gloves located by the doors)
  1. Decontaminate the workstation.
    1. Spray the keyboard, mouse and surrounding surfaces with 70% EtOH let sit 5 minutes. Wipe dry with a paper towel and discard in the trash. 
  2. Login and run your experiment.
  3. When finished, follow the posted instrument cleaning procedure. In addition, decontaminate the workstation again.
    1. Lightly spray the keyboard, mouse and surrounding surfaces with EtOH
    2. Let sit for 5 minutes
    3. Wipe dry with paper towels, discard in the trash.
  4. Remove gloves and discard before leaving the instrumentation rooms.

Data analysis workstation:

  1. Sanitize hands 1st before sitting down at a workstation in the Computer lab.
  1. Decontaminate the workstation.
    1. Spray the keyboard, mouse and surrounding surfaces with 70% EtOH let sit 5 minutes. Wipe dry with a paper towel and discard in the trash. 
  2. When finished, decontaminate the workstation again.
    1. Spray the same surfaces with 70% EtOH and wipe dry with paper towels, discard in the trash.

New Rates effective September 01, 2020

Sorter Rates are seeing an increase this year due to the limitations with COVID-19 and assited sorting from mid March until June. The Cell Analsysis Faciltiy is a recharge center and by federal law cannot make a profit and must cover all of its operational expenses by the rates charged to the users.. If you have questions, please contact Michele.

Check out our Latest Newsletter!

Other News

Users can now Subscribe to facility Announcements: Using the new online calendar, users can now subscribe to general announcements or subscribe to specific instrument updates that will be sent via email. This will provide timely information and alerts. Users are free to sign up for one or all lists that are of an interest. Instructions on how to subscribe are here.

Scheduling Calendar: You will need to login with you UWNetID to schedule time. If you do not have a UWNetID or have not created an account with the facility, please see Michele Black to be added to the access list. See Scheduling for more detailed information.

Any material requiring BSL-2 containment or any viable cells that could be classified as BSL-2 (this is all human cell lines and primary human samples ) must be cleared through EH&S by submitting a Biological Use Authorization form (BUA) form prior to being sorted at the facility.

When signing up for a sort please indicates the cells origin and you must specify the Biosafety level of your material. If it falls under BSL-2 you may not sort unless your lab is approved.  

Approval will be given on a case by case basis through IBS commitee and EH&S depending on the risk assessment as it applies specifically to cell sorting in the Cell Analysis Facility. Contact ehsbio@uw.edu to obtain the necessary forms and instructions to add the Cell Analysis Facility to your approved protocol.

Please refere to Sorting Requirements for additional information.

 

If you have any questions or any concerns please contact Michele Black at mblack2@uw.edu
or  685-3014.

 

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