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Sterilization Services

HSAS&F

HSLS Standard Operating Procedures (SOP)*

HS Laboratory Services provides sterilization of:

You can deliver materials between 8:00am – 5:00pm to T-276.

 

Contacts

Zemeheret Seare

Sophia Myers

hslabs@nulluw.edu

206-543-8869

Sterilization and Disposal of Biohazardous Waste

HS Laboratory Services (HSLS) has one large steam sterilizer dedicated to the sterilization of biohazardous waste with a 60 minute cycle at 121° C. The last load is run at 3:15 pm. If it is important that your waste run same-day, please bring it to HSLS as early as possible.

 

PACKING PROCEDURES

Please review the below packing procedures to ensure that deliveries to HSLS meet criteria prior to drop-off.

  • Solid biohazardous waste must be packaged in an autoclavable biohazard bag
    • no larger than 19 x 23
    • attach autoclave tape
    • clearly label with the Principal Investigator’s name and the room number
    • transport in a leak-proof secondary container
    • place in secondary bin upon arrival at HSLS.
  • Sharps waste and broken glassware must be packaged in red sharps containers
    • no larger than 11 x 13 x 16
    • no more than two-thirds full**
    • marked with biohazardous symbol
    • attach autoclave tape
    • clearly label with the Principal Investigator’s name and the room number
    • place in secondary bin upon arrival at HSLS.

General information on packaging sharps

  • Liquid waste must be packaged in a leak-proof, rigid container
    • no taller than 11.5 inches
    • no more than two-thirds full**
    • clearly label with the Principal Investigator’s name and the room number
    • transport in a leak-proof secondary container
  • Laboratory glass and plastic waste contaminated with biohazardous material should be packaged in a container that is easily autoclaved that does not allow the tips to puncture it (one possible solution is to use a pipette box) and
    • no larger than 11 x 15 x 21
    • attach autoclave tape
    • clearly label with the Principal Investigator’s name and the room number
    • transport in a leak-proof secondary container

General information on packaging lab glass and plastic

Not all plastic materials are safe for autoclaving. Polypropylene (PP) and polypropylene copolymer (PPCO) containers can be autoclaved many times. Fluoropolymer products, such as Teflon PFA, FEP, or ETFE, can also be autoclaved. However, Polycarbonate (PC) containers may withstand only 30 – 50 autoclaving cycles or less. The following resins cannot be autoclaved – HDPE, LDPE, PET, PETG. To maximize the longevity of reusable plastic containers suitable for the autoclave, HSLS recommends purchasing containers made from PP and PPCO material.

Due to a variance in the lifecycle of materials, HSLS will not replace plastic containers damaged during the autoclaving process.

 
DROP-OFF PROCEDURES

The following drop-off procedures must be followed to ensure the timely processing of Biohazardous Waste.

In order to receive services, customers are required to fill out the on-site sign-in sheet at the time of each drop-off. Customers are asked to provide the following information:

  • Date of Service
  • Laboratory Name
  • Room Number of Laboratory
  • Phone Number
  • Budget Number
  • Department
  • Building Code

Please drop off waste in T-276 by loading materials into plastic trays on the processing cart, filling up the lower shelves of the cart first. Each filled plastic tray is considered one load. We can process 9 loads of biohazardous waste at a time. The Magnuson Health Sciences Center Biohazardous Waste Flow Chart (pdf) summarizes the proper packaging, treatment and disposal of biological and biomedical waste for locations within Magnuson Health Sciences, W.H. Foege Genome Sciences, and W.H. Foege Bioengineering.


Sterilization of Lab Supplies

 

DROP-OFF/PICK-UP PROCEDURES

The following drop-off/pick-up procedures must be followed to ensure the sterilization of lab supplies.

In order to receive services, customers are required to fill out the on-site sign-in sheet at the time of each drop-off. Customers are asked to provide the following information:

  • Date of Service
  • Laboratory Name
  • Room Number of Laboratory
  • Phone Number
  • Budget Number
  • Department
  • Building Code

Reagents and Laboratory Supplies

HSLS has two autoclaves dedicated to the steam sterilization of reagents and laboratory supplies, such as pipette tips, caps, mixed glassware, and instruments. Please drop off supplies in T-276, loading your supplies onto a small autoclave cart. Do not overfill, item on carts may only be one layer. Special exposure times and temperatures can be requested. Once autoclaved these can be picked up from T-286.

Cell Culture Work

For those laboratories doing cell culture work, HSLS has an oven available to do high-heat dry sterilization. Please drop off materials in T-276 to be processed for 3 hours at 195 C. These loads are run at the end of the day and are then available to be picked up the next day from T-286.

*To ensure the safety of the Biowaste team, HS Laboratory Services (HSLS) cannot accept items that do not meet SOP. Items not meeting HSLS standards will be refused or sent back. If this occurs, the following can be completed to ensure proper disposal of Biowaste.

  • Repackage the item, ensuring that packaging does not exceed HSLS size requirements. For example, if a sharps container is brought to the HSLS facility too full, it can be placed directly into a larger sharps container and processed. Do not attempt to remove items from a sharps container.
  • If an item cannot be repackaged according to HSLS size requirements, it will need to be shipped to ensure proper disposal. To ship biohazardous waste, labs must:
    • Attend the “Shipping Regulated Medical Waste by Contracted Carrier” in-person training. See EH&S Training Website for more details. http://www.ehs.washington.edu/training/shipping-regulated-medical-waste-contracted-carrier
    • Sign up for waste pickup either through laboratory services (recommended) at biowaste@nulluw.edu or by filling out the online form at https://depts.washington.edu/hsasf/instructional-support/labservices/biowaste-collection/bio-hazardous-waste-collection-request-form/

If questions arise regarding the packaging or shipping of Biowaste, please contact UW EH&S at ehsbio@nulluw.edu or 206-221-7770 for support. You can also visit their website for more information on Biohazardous Waste and Sharps and Laboratory Glass

**This allows steam to reach the contents and allows for expansion and contraction of the container during autoclaving, which works to prevent sharps from puncturing the container during processing.