{"id":161,"date":"2015-09-28T21:47:43","date_gmt":"2015-09-28T21:47:43","guid":{"rendered":"https:\/\/depts.washington.edu\/jaisril\/?page_id=161"},"modified":"2019-11-05T23:02:47","modified_gmt":"2019-11-05T23:02:47","slug":"former-research-scientists","status":"publish","type":"page","link":"https:\/\/depts.washington.edu\/jaisril\/lab-members\/former-research-scientists\/","title":{"rendered":"Former Research Scientists (since 2005)"},"content":{"rendered":"

Daryl Phuong<\/h3>\n

\"Daryl<\/p>\n

Daryl was a Research Scientist in the Lingappa lab from 2015 – 2019. Daryl grew up in the Seattle area and attended Aviation High School (Now Raisbeck Aviation High School), which uses project-based learning to prepare students for further study in STEM related fields. He graduated from the University of Washington (UW) in 2015 with degrees in chemistry and biochemistry. His first exposure to the research world was in Susan Brockerhoff’s Lab at UW, where he learned to appreciate model organisms and their importance in studying genetic diseases. After graduating from UW, he worked in a clinical lab derivatizing drugs and learning about their extractions and purification techniques. Daryl left the Lingappa Lab in 2019 to join a PhD program at Cornell University in Ithaca, NY.<\/p>\n

Research:<\/h5>\n

In his first few months in the Lingappa lab, Daryl rapidly learned the basics of recombinant DNA technology.\u00a0 Very soon, he started learning new techniques and soon introduced an impressive array of new approaches to the Lingappa lab, including Gibson Assembly.\u00a0 He became an expert in Gibson Assembly, and even developed a very cost-efficient homemade Gibson Assembly kit that made improvements to the commercial kit.\u00a0 By greatly optimized our approach to mutating and modifying genomic-length retroviral plasmids, and generated an enormous number of new and useful plasmids during his time in the Lingappa lab.\u00a0 He also made significant improvements to our approach to recombinant protein expression and purification.<\/p>\n

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Kasana Chutiraka<\/h3>\n

\"Kasana_website_pic_2015\"Kasana grew up in Thailand, and came to Seattle for her undergraduate studies at the University of Washington, where she majored in biochemistry and psychology. As an undergraduate, she also enjoyed her courses in genetics and microbiology, and her volunteer work with HIV positive patients in Asia.\u00a0After graduating from UW, she worked as a medical scribe.\u00a0Her interest in seeing another side of infectious disease through research led her to a position in the Lingappa lab, where she was a Research Scientist from 2013-2016.<\/p>\n

Research:<\/h5>\n

During her time in the Lingappa lab, Kasana implemented two-step PCR approaches for generating proviral constructs.\u00a0She also learned immunoelectron microscopy (immunoEM) and helped generate many of the immunoEM images our lab generated.\u00a0She enjoys making novel observations using the electron microscope.<\/p>\n

Publications:<\/h5>\n

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    \n
  1. Barajas, BC, Tanaka, M, Robinson, BA, Phuong, DJ, Chutiraka, K, Reed, JC, Lingappa, JR<\/strong>. Identifying the assembly intermediate in which Gag first associates with unspliced HIV-1 RNA suggests a novel model for HIV-1 RNA packaging. PLoS Pathog. 2018;14 (4):e1006977. doi: 10.1371\/journal.ppat.1006977<\/a>. PubMed PMID:29664940<\/a> PubMed Central PMC5940231<\/a>.<\/li>\n
  2. Sette, P, O'Connor, SK, Yerramilli, VS, Dussupt, V, Nagashima, K, Chutiraka, K, Lingappa, J, Scarlata, S, Bouamr, F<\/strong>. HIV-1 Nucleocapsid Mimics the Membrane Adaptor Syntenin PDZ to Gain Access to ESCRTs and Promote Virus Budding. Cell Host Microbe. 2016;19 (3):336-48. doi: 10.1016\/j.chom.2016.02.004<\/a>. PubMed PMID:26962944<\/a> PubMed Central PMC4804359<\/a>.<\/li>\n
  3. Tanaka, M, Robinson, BA, Chutiraka, K, Geary, CD, Reed, JC, Lingappa, JR<\/strong>. Mutations of Conserved Residues in the Major Homology Region Arrest Assembling HIV-1 Gag as a Membrane-Targeted Intermediate Containing Genomic RNA and Cellular Proteins. J Virol. 2016;90 (4):1944-63. doi: 10.1128\/JVI.02698-15<\/a>. PubMed PMID:26656702<\/a> PubMed Central PMC4734008<\/a>.<\/li>\n
  4. Lingappa, JR, Reed, JC, Tanaka, M, Chutiraka, K, Robinson, BA<\/strong>. How HIV-1 Gag assembles in cells: Putting together pieces of the puzzle. Virus Res. 2014;193 :89-107. doi: 10.1016\/j.virusres.2014.07.001<\/a>. PubMed PMID:25066606<\/a> PubMed Central PMC4351045<\/a>.<\/ol>\n

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    <\/a>Clair Geary<\/h3>\n

    \"ClairClair Geary graduated from Scripps College in 2010 with a B.A. in Molecular Biology. As an undergraduate she worked on vaccine development for Rift Valley Fever at the Marquet\/Ferre Vaccine Research Center\u00a0at Pitzer College. During her two years in the Lingappa lab, she constructed complex proviral plasmids, learned to perform immunogold electron microscopy, and developed expertise in capturing mages using an electron microscope. She also contributed to a project studying how cellular \u201cHIV assembly machines\u201d promote viral particle production and HIV pathogenesis.\u00a0In 2013, Clair joined the Weill Cornell Graduate School in New York City to pursue her Ph.D. studies in the laboratory of Dr. Joseph Sun.<\/p>\n

    Publications:<\/h5>\n

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      \n
    1. Docampo, MD, da Silva, MB, Lazrak, A, Nichols, KB, Lieberman, SR, Slingerland, AE, Armijo, GK, Shono, Y, Nguyen, C, Monette, S, Dwomoh, E, Lee, N, Geary, CD, Perobelli, SM, Smith, M, Markey, KA, Vardhana, SA, Kousa, AI, Zamir, E, Greenfield, I, Sun, JC, Cross, JR, Peled, JU, Jenq, RR, Stein-Thoeringer, CK, van den Brink, MRM<\/strong>. Alloreactive T cells deficient of the short-chain fatty acid receptor GPR109A induce less graft-versus-host disease. Blood. 2022;139 (15):2392-2405. doi: 10.1182\/blood.2021010719<\/a>. PubMed PMID:34653248<\/a> PubMed Central PMC9012131<\/a>.<\/li>\n
    2. Wiedemann, GM, Geary, CD, Lau, CM, Sun, JC<\/strong>. Cutting Edge: STAT1-Mediated Epigenetic Control of Rsad2<\/i> Promotes Clonal Expansion of Antiviral NK Cells. J Immunol. 2020;205 (1):21-25. doi: 10.4049\/jimmunol.2000086<\/a>. PubMed PMID:32461239<\/a> PubMed Central PMC7314649<\/a>.<\/li>\n
    3. Adams, NM, Geary, CD, Santosa, EK, Lumaquin, D, Le Luduec, JB, Sottile, R, van der Ploeg, K, Hsu, J, Whitlock, BM, Jackson, BT, Weizman, OE, Huse, M, Hsu, KC, Sun, JC<\/strong>. Cytomegalovirus Infection Drives Avidity Selection of Natural Killer Cells. Immunity. 2019;50 (6):1381-1390.e5. doi: 10.1016\/j.immuni.2019.04.009<\/a>. PubMed PMID:31103381<\/a> PubMed Central PMC6614060<\/a>.<\/li>\n
    4. Geary, CD, Krishna, C, Lau, CM, Adams, NM, Gearty, SV, Pritykin, Y, Thomsen, AR, Leslie, CS, Sun, JC<\/strong>. Non-redundant ISGF3 Components Promote NK Cell Survival in an Auto-regulatory Manner during Viral Infection. Cell Rep. 2018;24 (8):1949-1957.e6. doi: 10.1016\/j.celrep.2018.07.060<\/a>. PubMed PMID:30134157<\/a> PubMed Central PMC6153266<\/a>.<\/li>\n
    5. Lau, CM, Adams, NM, Geary, CD, Weizman, OE, Rapp, M, Pritykin, Y, Leslie, CS, Sun, JC<\/strong>. Epigenetic control of innate and adaptive immune memory. Nat Immunol. 2018;19 (9):963-972. doi: 10.1038\/s41590-018-0176-1<\/a>. PubMed PMID:30082830<\/a> PubMed Central PMC6225771<\/a>.<\/li>\n
    6. Adams, NM, Lau, CM, Fan, X, Rapp, M, Geary, CD, Weizman, OE, Diaz-Salazar, C, Sun, JC<\/strong>. Transcription Factor IRF8 Orchestrates the Adaptive Natural Killer Cell Response. Immunity. 2018;48 (6):1172-1182.e6. doi: 10.1016\/j.immuni.2018.04.018<\/a>. PubMed PMID:29858012<\/a> PubMed Central PMC6233715<\/a>.<\/li>\n
    7. Madera, S, Geary, CD, Lau, CM, Pikovskaya, O, Reiner, SL, Sun, JC<\/strong>. Cutting Edge: Divergent Requirement of T-Box Transcription Factors in Effector and Memory NK Cells. J Immunol. 2018;200 (6):1977-1981. doi: 10.4049\/jimmunol.1700416<\/a>. PubMed PMID:29440505<\/a> PubMed Central PMC5840019<\/a>.<\/li>\n
    8. Rapp, M, Lau, CM, Adams, NM, Weizman, OE, O'Sullivan, TE, Geary, CD, Sun, JC<\/strong>. Core-binding factor β and Runx transcription factors promote adaptive natural killer cell responses. Sci Immunol. 2017;2 (18):. doi: 10.1126\/sciimmunol.aan3796<\/a>. PubMed PMID:29222089<\/a> PubMed Central PMC6265048<\/a>.<\/li>\n
    9. Geary, CD, Sun, JC<\/strong>. Memory responses of natural killer cells. Semin Immunol. 2017;31 :11-19. doi: 10.1016\/j.smim.2017.08.012<\/a>. PubMed PMID:28863960<\/a> PubMed Central PMC5724965<\/a>.<\/li>\n
    10. Adams, NM, O'Sullivan, TE, Geary, CD, Karo, JM, Amezquita, RA, Joshi, NS, Kaech, SM, Sun, JC<\/strong>. NK Cell Responses Redefine Immunological Memory. J Immunol. 2016;197 (8):2963-2970. doi: 10.4049\/jimmunol.1600973<\/a>. PubMed PMID:27824591<\/a> PubMed Central PMC5108457<\/a>.<\/li>\n
    11. O'Sullivan, TE, Geary, CD, Weizman, OE, Geiger, TL, Rapp, M, Dorn, GW 2nd, Overholtzer, M, Sun, JC<\/strong>. Atg5 Is Essential for the Development and Survival of Innate Lymphocytes. Cell Rep. 2016;15 (9):1910-9. doi: 10.1016\/j.celrep.2016.04.082<\/a>. PubMed PMID:27210760<\/a> PubMed Central PMC4889506<\/a>.<\/li>\n
    12. Tanaka, M, Robinson, BA, Chutiraka, K, Geary, CD, Reed, JC, Lingappa, JR<\/strong>. Mutations of Conserved Residues in the Major Homology Region Arrest Assembling HIV-1 Gag as a Membrane-Targeted Intermediate Containing Genomic RNA and Cellular Proteins. J Virol. 2016;90 (4):1944-63. doi: 10.1128\/JVI.02698-15<\/a>. PubMed PMID:26656702<\/a> PubMed Central PMC4734008<\/a>.<\/li>\n
    13. Geiger, TL, Abt, MC, Gasteiger, G, Firth, MA, O'Connor, MH, Geary, CD, O'Sullivan, TE, van den Brink, MR, Pamer, EG, Hanash, AM, Sun, JC<\/strong>. Nfil3 is crucial for development of innate lymphoid cells and host protection against intestinal pathogens. J Exp Med. 2014;211 (9):1723-31. doi: 10.1084\/jem.20140212<\/a>. PubMed PMID:25113970<\/a> PubMed Central PMC4144732<\/a>.<\/li>\n
    14. Robinson, BA, Reed, JC, Geary, CD, Swain, JV, Lingappa, JR<\/strong>. A temporospatial map that defines specific steps at which critical surfaces in the Gag MA and CA domains act during immature HIV-1 capsid assembly in cells. J Virol. 2014;88 (10):5718-41. doi: 10.1128\/JVI.03609-13<\/a>. PubMed PMID:24623418<\/a> PubMed Central PMC4019110<\/a>.<\/li>\n
    15. Reed, JC, Molter, B, Geary, CD, McNevin, J, McElrath, J, Giri, S, Klein, KC, Lingappa, JR<\/strong>. HIV-1 Gag co-opts a cellular complex containing DDX6, a helicase that facilitates capsid assembly. J Cell Biol. 2012;198 (3):439-56. doi: 10.1083\/jcb.201111012<\/a>. PubMed PMID:22851315<\/a> PubMed Central PMC3413349<\/a>.<\/ol>\n

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      <\/a>J. Victor Swain<\/h3>\n

      \"Vic_June2012.img_assist_custom-180x135\"<\/p>\n

      J. Victor Swain graduated from Drexel University in 1998 with a B.S. in Biotechnology. \u00a0He has worked in industry, including at GlaxoSmithKline and Merck, and in the Mullins lab at the University of Washington. \u00a0He joined the Lingappa lab as a Research Scientist in 2012, moved to the Seattle Prosetta Lab in 2013.<\/p>\n

      Publications:<\/h5>\n

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        \n
      1. Reed, JC, Solas, D, Kitaygorodskyy, A, Freeman, B, Ressler, DTB, Phuong, DJ, Swain, JV, Matlack, K, Hurt, CR, Lingappa, VR, Lingappa, JR<\/strong>. Identification of an Antiretroviral Small Molecule That Appears To Be a Host-Targeting Inhibitor of HIV-1 Assembly. J Virol. 2021;95 (3):. doi: 10.1128\/JVI.00883-20<\/a>. PubMed PMID:33148797<\/a> PubMed Central PMC7925099<\/a>.<\/li>\n
      2. Reed, JC, Westergreen, N, Barajas, BC, Ressler, DTB, Phuong, DJ, Swain, JV, Lingappa, VR, Lingappa, JR<\/strong>. Formation of RNA Granule-Derived Capsid Assembly Intermediates Appears To Be Conserved between Human Immunodeficiency Virus Type 1 and the Nonprimate Lentivirus Feline Immunodeficiency Virus. J Virol. 2018;92 (9):. doi: 10.1128\/JVI.01761-17<\/a>. PubMed PMID:29467316<\/a> PubMed Central PMC5899207<\/a>.<\/li>\n
      3. Manocheewa, S, Lanxon-Cookson, EC, Liu, Y, Swain, JV, McClure, J, Rao, U, Maust, B, Deng, W, Sunshine, JE, Kim, M, Rolland, M, Mullins, JI<\/strong>. Pairwise growth competition assay for determining the replication fitness of human immunodeficiency viruses. J Vis Exp. 2015; (99):e52610. doi: 10.3791\/52610<\/a>. PubMed PMID:25993602<\/a> PubMed Central PMC4542137<\/a>.<\/li>\n
      4. Robinson, BA, Reed, JC, Geary, CD, Swain, JV, Lingappa, JR<\/strong>. A temporospatial map that defines specific steps at which critical surfaces in the Gag MA and CA domains act during immature HIV-1 capsid assembly in cells. J Virol. 2014;88 (10):5718-41. doi: 10.1128\/JVI.03609-13<\/a>. PubMed PMID:24623418<\/a> PubMed Central PMC4019110<\/a>.<\/li>\n
      5. Lanxon-Cookson, EC, Swain, JV, Manocheewa, S, Smith, RA, Maust, B, Kim, M, Westfall, D, Rolland, M, Mullins, JI<\/strong>. Factors affecting relative fitness measurements in pairwise competition assays of human immunodeficiency viruses. J Virol Methods. 2013;194 (1-2):7-13. doi: 10.1016\/j.jviromet.2013.07.062<\/a>. PubMed PMID:23933395<\/a> PubMed Central PMC3825816<\/a>.<\/li>\n
      6. Manocheewa, S, Swain, JV, Lanxon-Cookson, E, Rolland, M, Mullins, JI<\/strong>. Fitness costs of mutations at the HIV-1 capsid hexamerization interface. PLoS One. 2013;8 (6):e66065. doi: 10.1371\/journal.pone.0066065<\/a>. PubMed PMID:23785468<\/a> PubMed Central PMC3681919<\/a>.<\/li>\n
      7. Rolland, M, Manocheewa, S, Swain, JV, Lanxon-Cookson, EC, Kim, M, Westfall, DH, Larsen, BB, Gilbert, PB, Mullins, JI<\/strong>. HIV-1 conserved-element vaccines: relationship between sequence conservation and replicative capacity. J Virol. 2013;87 (10):5461-7. doi: 10.1128\/JVI.03033-12<\/a>. PubMed PMID:23468488<\/a> PubMed Central PMC3648173<\/a>.<\/li>\n
      8. Liu, Y, Holte, S, Rao, U, McClure, J, Konopa, P, Swain, JV, Lanxon-Cookson, E, Kim, M, Chen, L, Mullins, JI<\/strong>. A sensitive real-time PCR based assay to estimate the impact of amino acid substitutions on the competitive replication fitness of human immunodeficiency virus type 1 in cell culture. J Virol Methods. 2013;189 (1):157-66. doi: 10.1016\/j.jviromet.2012.10.016<\/a>. PubMed PMID:23201292<\/a> PubMed Central PMC3608845<\/a>.<\/li>\n
      9. Rolland, M, Carlson, JM, Manocheewa, S, Swain, JV, Lanxon-Cookson, E, Deng, W, Rousseau, CM, Raugi, DN, Learn, GH, Maust, BS, Coovadia, H, Ndung'u, T, Goulder, PJ, Walker, BD, Brander, C, Heckerman, DE, Mullins, JI<\/strong>. Amino-acid co-variation in HIV-1 Gag subtype C: HLA-mediated selection pressure and compensatory dynamics. PLoS One. 2010;5 (9):. doi: 10.1371\/journal.pone.0012463<\/a>. PubMed PMID:20824187<\/a> PubMed Central PMC2931691<\/a>.<\/li>\n
      10. van 't Wout, AB, Swain, JV, Schindler, M, Rao, U, Pathmajeyan, MS, Mullins, JI, Kirchhoff, F<\/strong>. Nef induces multiple genes involved in cholesterol synthesis and uptake in human immunodeficiency virus type 1-infected T cells. J Virol. 2005;79 (15):10053-8. doi: 10.1128\/JVI.79.15.10053-10058.2005<\/a>. PubMed PMID:16014965<\/a> PubMed Central PMC1181597<\/a>.<\/ol>\n

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        <\/a>Veronica Nguyen<\/h3>\n

        \"VeronicaVeronica Nguyen completed her bachelor of science in Economics at the University of Washington in 2009.\u00a0 Veronica is also a co-founding member of the UW chapter of Global Medical Brigades, an international student-led volunteer health care relief organization in Honduras, Panama and Ghana.\u00a0 She began working in the Lingappa lab in 2007 as a student assistant and is was hired as a research scientist in 2009.\u00a0 In the Lingappa lab, she constructed plasmids, maintained our plasmid library, helped with electron microscopy studies, and performed a wide variety of lab support duties.\u00a0 In the summer of 2011, she took a short leave of absence to be involved in an HIV education and awareness project in Tanzania, Africa.<\/p>\n

        Veronica left the Lingappa lab in the summer of 2011 to pursue her studies as a medical student at Albany Medical College.\u00a0 Subsequently, she was an internal medicine resident at Georgetown University Hospital in Washington, DC, and a fellow in the Dept. of Medicine at the University of Arizona Health Sciences campus in Tucson.<\/p>\n

        Publications:<\/h5>\n

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          \n
        1. Klein KC, Reed JC, Tanaka M, Nguyen V, Giri S, and Lingappa JR. \u00a0\u00a0HIV Gag-Leucine zipper chimeras form ABCE1-containing intermediates and RNAse-resistant immature capsids similar to those formed by wild-type HIV-1 Gag.\u00a0\u00a0Journal of Virology, 2011 Jul;85(14):7419-35. [Download]<\/a><\/ol>\n

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          <\/a>Samina Giri<\/h3>\n

          \"SaminaSamina Giri grew up in Los Angeles and Tucson and obtained her B.S. degree in Molecular and Cellular Biology from University of Arizona in Tucson.\u00a0 In Tucson she worked on BAC library construction and ancestral DNA studies. She was a member of the Lingappa Lab from 2009 – 2011.<\/p>\n

          Research:<\/h5>\n

          During her time in the Lingappa lab, Samina specialized\u00a0 in performing immunogold labeling electron microscopy. \u00a0 She worked closely with the staff at the Fred Hutchinson Cancer Research Center Electron Microscopy Facility.<\/p>\n

          Publications:<\/h5>\n

          <\/p>\n

            \n
          1. Reed JC, Molter B, Geary CD, McNevin J, McElrath J, Giri S, Klein KC, Lingappa JR.\u00a0 HIV-1 Gag co-opts a cellular complex containing DDX6, a helicase that facilitates capsid assembly.\u00a0 J Cell Biol. 2012 Aug 6;198(3):439-56. Epub 2012 Jul 30.[Download]<\/a><\/span>\n<\/li>\n
          2. Klein KC, Reed JC, Tanaka M, Nguyen V, Giri S, and Lingappa JR. \u00a0\u00a0HIV Gag-Leucine zipper chimeras form ABCE1-containing intermediates and RNAse-resistant immature capsids similar to those formed by wild-type HIV-1 Gag.\u00a0\u00a0Journal of Virology, 2011 Jul;85(14):7419-35. [Download]<\/a><\/span><\/ol>\n

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            <\/a>Brook Barajas<\/h3>\n

            \"Brook<\/p>\n

            Brook Barajas\u00a0was raised in Seattle, WA. She majored in Microbiology at the University of California at Santa Barbara (UCSB), where she graduated with Highest Honors in 2007 and was the Student Commencement Speaker. As an undergraduate, she was frequently on the Dean\u2019s list and received numerous honors for her academic scholarship, including an award for \u201cOutstanding Critical Thinking and Analysis in the Laboratory\u201d. While at UCSB, she worked in the laboratory of Dr. Kevin Plaxco in the UCSB Dept. of Chemistry.\u00a0 Brook was a research scientist in the Lingappa laboratory from 2007 \u2013 2009.\u00a0 She left the Lingappa lab to join the Cancer Biology Ph.D. program at Stanford University, where she was awarded a Stanford Graduate Fellowship in 2009 and an NSF Graduate Research Fellowship in 2011.\u00a0 Her Ph.D. dissertation studies were in the laboratory of\u00a0Dr. Paul Khavari, in the Dept. of Dermatology at Stanford University, where she is leading a project on epigenomic profiling in differentiating primary epidermal keratinocytes.\u00a0 She returned to the Lingappa lab in 2016 for her postdoctoral studies and subsequently joined Juno Therapeutics in Seattle.<\/p>\n

            Research:<\/h5>\n

            Brook worked closely with\u00a0Beth Thielen<\/a>\u00a0in the Lingappa lab, studying regulation of the deaminase activity of various APOBEC3 proteins expressed in primary human cells.<\/p>\n

            Publications:<\/h5>\n

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              \n
            1. Suker, A, Li, Y, Robson, D, Marren, A, Australasian CREI (Certificate of Reproductive Endocrinology and Infertility) Consensus Expert Panel on Trial Evidence (ACCEPT) Group<\/strong>. Australasian recurrent pregnancy loss clinical management guideline 2024, part II. Aust N Z J Obstet Gynaecol. 2024;64 (5):445-458. doi: 10.1111\/ajo.13820<\/a>. PubMed PMID:38934293<\/a> PubMed Central PMC11660019<\/a>.<\/li>\n
            2. Rubin, AJ, Barajas, BC, Furlan-Magaril, M, Lopez-Pajares, V, Mumbach, MR, Howard, I, Kim, DS, Boxer, LD, Cairns, J, Spivakov, M, Wingett, SW, Shi, M, Zhao, Z, Greenleaf, WJ, Kundaje, A, Snyder, M, Chang, HY, Fraser, P, Khavari, PA<\/strong>. Lineage-specific dynamic and pre-established enhancer-promoter contacts cooperate in terminal differentiation. Nat Genet. 2017;49 (10):1522-1528. doi: 10.1038\/ng.3935<\/a>. PubMed PMID:28805829<\/a> PubMed Central PMC5715812<\/a>.<\/li>\n
            3. Lopez-Pajares, V, Qu, K, Zhang, J, Webster, DE, Barajas, BC, Siprashvili, Z, Zarnegar, BJ, Boxer, LD, Rios, EJ, Tao, S, Kretz, M, Khavari, PA<\/strong>. A LncRNA-MAF:MAFB transcription factor network regulates epidermal differentiation. Dev Cell. 2015;32 (6):693-706. doi: 10.1016\/j.devcel.2015.01.028<\/a>. PubMed PMID:25805135<\/a> PubMed Central PMC4456036<\/a>.<\/li>\n
            4. Boxer, LD, Barajas, B, Tao, S, Zhang, J, Khavari, PA<\/strong>. ZNF750 interacts with KLF4 and RCOR1, KDM1A, and CTBP1\/2 chromatin regulators to repress epidermal progenitor genes and induce differentiation genes. Genes Dev. 2014;28 (18):2013-26. doi: 10.1101\/gad.246579.114<\/a>. PubMed PMID:25228645<\/a> PubMed Central PMC4173152<\/a>.<\/li>\n
            5. Webster, DE, Barajas, B, Bussat, RT, Yan, KJ, Neela, PH, Flockhart, RJ, Kovalski, J, Zehnder, A, Khavari, PA<\/strong>. Enhancer-targeted genome editing selectively blocks innate resistance to oncokinase inhibition. Genome Res. 2014;24 (5):751-60. doi: 10.1101\/gr.166231.113<\/a>. PubMed PMID:24443471<\/a> PubMed Central PMC4009605<\/a>.<\/li>\n
            6. Zarnegar, BJ, Webster, DE, Lopez-Pajares, V, Vander Stoep Hunt, B, Qu, K, Yan, KJ, Berk, DR, Sen, GL, Khavari, PA<\/strong>. Genomic profiling of a human organotypic model of AEC syndrome reveals ZNF750 as an essential downstream target of mutant TP63. Am J Hum Genet. 2012;91 (3):435-43. doi: 10.1016\/j.ajhg.2012.07.007<\/a>. PubMed PMID:22922031<\/a> PubMed Central PMC3511987<\/a>.<\/li>\n
            7. Thielen, BK, McNevin, JP, McElrath, MJ, Hunt, BV, Klein, KC, Lingappa, JR<\/strong>. Innate immune signaling induces high levels of TC-specific deaminase activity in primary monocyte-derived cells through expression of APOBEC3A isoforms. J Biol Chem. 2010;285 (36):27753-66. doi: 10.1074\/jbc.M110.102822<\/a>. PubMed PMID:20615867<\/a> PubMed Central PMC2934643<\/a>.<\/li>\n
            8. Lubin, AA, Hunt, BV, White, RJ, Plaxco, KW<\/strong>. Effects of probe length, probe geometry, and redox-tag placement on the performance of the electrochemical E-DNA sensor. Anal Chem. 2009;81 (6):2150-8. doi: 10.1021\/ac802317k<\/a>. PubMed PMID:19215066<\/a> .<\/ol>\n

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              <\/a>Britta Molter<\/h3>\n

              \"BrittaBritta grew up in Warendorf, a small town in northwestern Germany.\u00a0 She studied biology at the Westfalian Wilhelm\u2018s University of M\u00fcnster. In 2003, she received the ERASMUS Mundus Scholarship to study abroad in Trondheim, Norway. She was granted the DAAD (Deutscher Akademischer Austauschdienst) Scholarshipto conduct her master\u2019s thesis research at the University of Washington in 2005. For her master\u2019s thesis, she investigated the behavior of Malayan sun bears in captivity and correlated it to social conditions and endocrine measurements. In 2006, she obtained her Master\u2019s degree in Biology, with emphases on behavioral biology and biochemistry.\u00a0 Britta was a research scientist in the Lingappa lab from 2006 \u2013 2008.\u00a0 In 2008, Britta returned to Germany to raise her children.<\/p>\n

              Research:<\/h5>\n

              Britta worked closely with Jonathan Reed to demonstrate that DDX6 plays a critical role in HIV-1 capsid assembly.<\/p>\n

              Publications:<\/h5>\n

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                \n
              1. Reed JC, Molter B, Geary CD, McNevin J, McElrath J, Giri S, Klein KC, Lingappa JR.\u00a0 HIV-1 Gag co-opts a cellular complex containing DDX6, a helicase that facilitates capsid assembly.\u00a0 J Cell Biol. 2012 Aug 6;198(3):439-56. Epub 2012 Jul 30.<\/span>\u00a0[Download]<\/a><\/ol>\n

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                <\/a>Lorne Walker<\/h3>\n

                \"LorneLorne Walker graduated from UW in June 2002 with a double major in Biochemistry and Applied and Computational Mathematical Sciences. He was a technician in the Lingappa lab from 2002 \u2013 2005 and was involved in a wide variety of projects. \u00a0He left the Lingappa lab to join the MD PhD program at UC San Diego and went on to do his residency in the pediatrics program at the Children’s Hospital of Pittsburgh (University of Pittsburgh Medical Center).<\/p>\n

                Publications:<\/h5>\n

                <\/p>\n

                  \n
                1. Thielen BK, Klein KC, Walker LW, Rieck M, Buckner JH, Tomblingson GW, and Lingappa JR. T cells contain an RNase-insensitive inhibitor of APOBEC3G deaminase activity. PLoS Pathogens, accepted June 2007.<\/ol>\n

                   <\/p>\n

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                   <\/p>\n

                  Mona Dellos<\/h3>\n

                  \"MonaMona Dellos worked in the Lingappa lab while she was an undergraduate at UW. She worked closely with Kevin Klein, while he was a graduate student in the lab studying hepatitis C virus assembly.<\/p>\n

                  Publications:<\/h5>\n

                  <\/p>\n

                    \n
                  1. Klein, K. C., S. R. Dellos, and J. R. Lingappa. Identification of residues in the core protein that are critical for assembly of hepatitis C virus capsids using a cell-free system. Journal of Virology. 2005 Jun;79(11):6814-26. Erratum in: J Virol. 2005 Aug;79(15):10098.\t \t\t[ Abstract<\/a>]<\/ol>\n

                     <\/p>\n

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                     <\/p>\n

                    Garrett Tomblingson<\/h3>\n

                    \"GarrettGarrett was a research technician in the Lingappa Lab between August 2005 and June 2007.<\/p>\n

                    Publications:<\/h5>\n

                    <\/p>\n

                      \n
                    1. Thielen BK, Klein KC, Walker LW, Rieck M, Buckner JH, Tomblingson GW, and Lingappa JR. T cells contain an RNase-insensitive inhibitor of APOBEC3G deaminase activity. PLoS Pathogens, accepted June 2007.<\/ol>\n
                      Go to top<\/a><\/div>\n","protected":false},"excerpt":{"rendered":"

                      Daryl Phuong Daryl was a Research Scientist in the Lingappa lab from 2015 – 2019. Daryl grew up in the Seattle area and attended Aviation High School (Now Raisbeck Aviation High School), which uses project-based learning to prepare students for further study in STEM related fields. He graduated from the University of Washington (UW) in…<\/p>\n

                      Read more<\/a><\/div>\n","protected":false},"author":1,"featured_media":0,"parent":154,"menu_order":5,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-161","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/depts.washington.edu\/jaisril\/wp-json\/wp\/v2\/pages\/161","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/depts.washington.edu\/jaisril\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/depts.washington.edu\/jaisril\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/jaisril\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/jaisril\/wp-json\/wp\/v2\/comments?post=161"}],"version-history":[{"count":41,"href":"https:\/\/depts.washington.edu\/jaisril\/wp-json\/wp\/v2\/pages\/161\/revisions"}],"predecessor-version":[{"id":543,"href":"https:\/\/depts.washington.edu\/jaisril\/wp-json\/wp\/v2\/pages\/161\/revisions\/543"}],"up":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/jaisril\/wp-json\/wp\/v2\/pages\/154"}],"wp:attachment":[{"href":"https:\/\/depts.washington.edu\/jaisril\/wp-json\/wp\/v2\/media?parent=161"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}