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Lingappa, JR, Reed, JC, Tanaka, M, Chutiraka, K, Robinson, BA<\/strong>. How HIV-1 Gag assembles in cells: Putting together pieces of the puzzle. Virus Res. 2014;193 :89-107. doi: 10.1016\/j.virusres.2014.07.001<\/a>. PubMed PMID:25066606<\/a> PubMed Central PMC4351045<\/a>.<\/p>\n This review serves as an introduction to the field for newcomers, but also provides a useful summary to those more familiar with HIV-1 assembly.\u00a0 In it, we describe how different experimental approaches have been used to study assembly of the HIV-1 immature capsid \u2013 including analysis of mutations, structural biology, assembly of recombinant Gag, assembly in cell-free systems, imaging, biochemical approaches, and siRNA knockdowns.\u00a0 This review also highlights unanswered questions in the HIV-1 assembly field.<\/p>\n <\/p>\n <\/span><\/p>\n <\/p>\n Lingappa, UF, Wu, X, Macieik, A, Yu, SF, Atuegbu, A, Corpuz, M, Francis, J, Nichols, C, Calayag, A, Shi, H, Ellison, JA, Harrell, EK, Asundi, V, Lingappa, JR, Prasad, MD, Lipkin, WI, Dey, D, Hurt, CR, Lingappa, VR, Hansen, WJ, Rupprecht, CE<\/strong>. Host-rabies virus protein-protein interactions as druggable antiviral targets. Proc Natl Acad Sci U S A. 2013;110 (10):E861-8. doi: 10.1073\/pnas.1210198110<\/a>. PubMed PMID:23404707<\/a> PubMed Central PMC3593902<\/a>.<\/p>\n Rabies is almost always fatal and causes a significant number of deaths in developing countries; yet, antiviral drugs for treating the disease are completely lacking.\u00a0 In this paper, Prosetta Biosciences used an assembly pathway screen to identify a small molecule that inhibits rabies capsid assembly.\u00a0 This drug-like compound binds to complexes containing ABCE1, a cellular enzyme that Jaisri\u00a0Lingappa’s lab has implicated in facilitating\u00a0HIV-1 capsid assembly.\u00a0 Authors on this paper include investigators at Prosetta Biosciences, the Centers for Disease Control (CDC), and Jaisri\u00a0Lingappa’s lab at UW.<\/p>\n \u201cCell Leading Edge Select\u201d commentary on this paper, entitled Targeting the Host-Virus Machinery<\/a><\/p>\n Learn more about the rabies drug screen used in\u00a0this paper from\u00a0an article by science writer Carl Zimmer in the blog\u00a0Phenomena by National Geographic<\/a>,\u00a0and in a SciBx article<\/a>.<\/p>\n