{"id":255,"date":"2019-10-21T23:03:31","date_gmt":"2019-10-21T23:03:31","guid":{"rendered":"http:\/\/depts.washington.edu\/keck\/wordpress\/?page_id=255"},"modified":"2023-03-22T19:26:00","modified_gmt":"2023-03-22T19:26:00","slug":"confocal-microscopes","status":"publish","type":"page","link":"https:\/\/depts.washington.edu\/keck\/confocal-microscopes\/","title":{"rendered":"Confocal Microscopes"},"content":{"rendered":"\n<h3 class=\"wp-block-heading\">Laser-scanning Confocal Microscopes<\/h3>\n\n\n\n<p>The Keck Center&#8217;s laser-scanning confocal microscopes use laser excitation, motorized scanning mirrors, confocal pinholes, and amplification or photon-counting detectors to collect high-resolution optical sections that exclude out-of-focus light. &nbsp; Through serial, optical sectioning of fluorescently-labelled samples, these systems are capable of producing high-resolution 3D and 4D datasets.<\/p>\n\n\n\n<h4 class=\"wp-block-heading\">Leica SP8X Confocal<\/h4>\n\n\n\n<p><em>All publications that include data acquired on the Leica SP8X confocal must acknowledge <strong>NIH grant S10 OD016240<\/strong>.<\/em><\/p>\n\n\n\n<p><\/p>\n\n\n\n<ul class=\"wp-block-list\"><li>Leica DMI6000 microscope<\/li><li>Motorized XY stage capable of mosaic tiling and multi-point imaging <\/li><li>Motorized Z-galvo stage for rapid Z movement <\/li><li>10X and 20X dry objectives <\/li><li>40X and 63X oil immersion objectives <\/li><li>25X and 63X water immersion objectives <\/li><li>Adaptive focus control (AFC) for maintaining focus during live imaging and\/or mosaic tiling <\/li><li>470-670nm tunable White Light Laser (WLL) system<\/li><li>405, 458, 476, 488, 496, and 514nm fixed wavelength laser lines <\/li><li>3 PMT amplification detectors <\/li><li>2 photon-counting HyD detectors with lifetime gating <\/li><li>Trans-PMT detector for DIC imaging <\/li><li>Resonant scanner for rapid, live-imaging<\/li><li>STP8000 backlit touchpad <em>(funded by UWSTF)<\/em><\/li><li>KLONDIKE scan head electronics <em>(funded by UWSTF)<\/em><\/li><li>Expert CUDA Workstation <em>(funded by UWSTF)<\/em><\/li><li>Leica LASX Expert software<em> (funded by UWSTF)<\/em><ul><li>Navigator<\/li><li>FRAP<\/li><li>FRET<\/li><li>Lightning Deconvolution <em>(funded by UWSTF)<\/em><\/li><\/ul><\/li><\/ul>\n\n\n\n<p><em>KLONDIKE scan head electronics, CUDA computer workstation, and LASX Expert software with Lightning deconvolution were funded by the <a href=\"https:\/\/uwstf.org\/\">UW Student Technology Fee<\/a>.<\/em><\/p>\n\n\n\n<figure class=\"wp-block-image\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"768\" src=\"https:\/\/depts.washington.edu\/keck\/wordpress\/wp-content\/uploads\/2019\/10\/Leica-SP8X-Confocal-1024x768.jpg\" alt=\"\" class=\"wp-image-411\" srcset=\"https:\/\/depts.washington.edu\/keck\/wordpress\/wp-content\/uploads\/2019\/10\/Leica-SP8X-Confocal-1024x768.jpg 1024w, https:\/\/depts.washington.edu\/keck\/wordpress\/wp-content\/uploads\/2019\/10\/Leica-SP8X-Confocal-300x225.jpg 300w, https:\/\/depts.washington.edu\/keck\/wordpress\/wp-content\/uploads\/2019\/10\/Leica-SP8X-Confocal-768x576.jpg 768w, https:\/\/depts.washington.edu\/keck\/wordpress\/wp-content\/uploads\/2019\/10\/Leica-SP8X-Confocal-1600x1200.jpg 1600w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<figure class=\"wp-block-image\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"768\" src=\"https:\/\/depts.washington.edu\/keck\/wordpress\/wp-content\/uploads\/2019\/10\/Leica-SP8X-Confocal-2-1024x768.jpg\" alt=\"\" class=\"wp-image-418\" srcset=\"https:\/\/depts.washington.edu\/keck\/wordpress\/wp-content\/uploads\/2019\/10\/Leica-SP8X-Confocal-2-1024x768.jpg 1024w, https:\/\/depts.washington.edu\/keck\/wordpress\/wp-content\/uploads\/2019\/10\/Leica-SP8X-Confocal-2-300x225.jpg 300w, https:\/\/depts.washington.edu\/keck\/wordpress\/wp-content\/uploads\/2019\/10\/Leica-SP8X-Confocal-2-768x576.jpg 768w, https:\/\/depts.washington.edu\/keck\/wordpress\/wp-content\/uploads\/2019\/10\/Leica-SP8X-Confocal-2-1600x1200.jpg 1600w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<h4 class=\"wp-block-heading\">Zeiss LSM 710 Confocal<\/h4>\n\n\n\n<ul class=\"wp-block-list\"><li> Zeiss Axio Observer Z.1 microscope <\/li><li> Manual stage with slide, dish, and plate inserts <\/li><li> 5X, 10X and 20X dry objectives <\/li><li> 40X, 63X, and 100X(no UV) oil immersion objectives <\/li><li> 40X and 63X water immersion objectives <\/li><li> 405, 458, 488, 514, 561, 594, and 633nm fixed wavelength laser lines <\/li><li> 2 PMT amplification detectors <\/li><li> 1 spectral PMT amplification detector <\/li><li> Tunable emission for all detectors <\/li><li> Trans-PMT detector for DIC imaging <\/li><li> Zeiss Zen 2009 software <\/li><\/ul>\n\n\n\n<figure class=\"wp-block-image\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"768\" src=\"https:\/\/depts.washington.edu\/keck\/wordpress\/wp-content\/uploads\/2019\/10\/Zeiss-710-Confocal-1024x768.jpg\" alt=\"\" class=\"wp-image-414\" srcset=\"https:\/\/depts.washington.edu\/keck\/wordpress\/wp-content\/uploads\/2019\/10\/Zeiss-710-Confocal-1024x768.jpg 1024w, https:\/\/depts.washington.edu\/keck\/wordpress\/wp-content\/uploads\/2019\/10\/Zeiss-710-Confocal-300x225.jpg 300w, https:\/\/depts.washington.edu\/keck\/wordpress\/wp-content\/uploads\/2019\/10\/Zeiss-710-Confocal-768x576.jpg 768w, https:\/\/depts.washington.edu\/keck\/wordpress\/wp-content\/uploads\/2019\/10\/Zeiss-710-Confocal-1600x1200.jpg 1600w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n","protected":false},"excerpt":{"rendered":"<p>Laser-scanning Confocal Microscopes The Keck Center&#8217;s laser-scanning confocal microscopes use laser excitation, motorized scanning mirrors, confocal pinholes, and amplification or&hellip;<\/p>\n","protected":false},"author":4,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-255","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/depts.washington.edu\/keck\/wp-json\/wp\/v2\/pages\/255","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/depts.washington.edu\/keck\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/depts.washington.edu\/keck\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/keck\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/keck\/wp-json\/wp\/v2\/comments?post=255"}],"version-history":[{"count":14,"href":"https:\/\/depts.washington.edu\/keck\/wp-json\/wp\/v2\/pages\/255\/revisions"}],"predecessor-version":[{"id":889,"href":"https:\/\/depts.washington.edu\/keck\/wp-json\/wp\/v2\/pages\/255\/revisions\/889"}],"wp:attachment":[{"href":"https:\/\/depts.washington.edu\/keck\/wp-json\/wp\/v2\/media?parent=255"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}