{"id":258,"date":"2019-10-21T23:03:52","date_gmt":"2019-10-21T23:03:52","guid":{"rendered":"http:\/\/depts.washington.edu\/keck\/wordpress\/?page_id=258"},"modified":"2023-04-18T20:19:27","modified_gmt":"2023-04-18T20:19:27","slug":"widefield-microscopes","status":"publish","type":"page","link":"https:\/\/depts.washington.edu\/keck\/widefield-microscopes\/","title":{"rendered":"Widefield Microscopes"},"content":{"rendered":"\n<h3 class=\"wp-block-heading\">Widefield Microscopes<\/h3>\n\n\n\n<p>The Keck Center&#8217;s widefield microscope systems use fluorescent lamp excitation, filter cubes, and CCD monochrome and\/or RGB-color cameras to produce fluorescent, brightfield, DIC, Phase Contrast, and\/or RGB-color images.  The motorized XY-stage on the Leica DMI6000 microscope allows for mosaic tiling of large tissue sections, both fluorescent and color-stained, and multi-point live imaging. &nbsp;Widefield microscopes do not exclude out-of-focus light as scanning confocal microscopes do, so they are usually not suitable for imaging thick samples.<\/p>\n\n\n\n<p><\/p>\n\n\n\n<h4 class=\"wp-block-heading\">Leica Inverted Multi-Dimensional Widefield<\/h4>\n\n\n\n<ul class=\"wp-block-list\"><li>Leica DMI6000 inverted microscope <\/li><li>5X LWD, 10X LWD, 20X, 20X LWD and 40X LWD dry objectives<\/li><li>40X and 100X Oil objectives<\/li><li>63X water objective<\/li><li>BFP (blue), CFP (cyan), GFP (green), YFP (yellow), TexasRed (red), and CY5 (far red) filter cubes <\/li><li>Motorized filters, shutters, and Z-drive<\/li><li>Motorized stage for mosaic tiling and\/or multi-point live-imaging<\/li><li>DIC optics <\/li><li>Phase Contrast optics (for use with long working distance (LWD) objectives) <\/li><li>Z-sectioning<\/li><li>Mosaic tiling<\/li><li>Multi-point acquisition for live imaging<\/li><li>Leica DFC365 FX CCD camera (fluorescence, brightfield, DIC, phase contrast)<\/li><li>Leica MC170HD Color camera (color &#8211; e.g., crystal violet, histological stains)<\/li><li>LASX software with Navigator<br><br><\/li><\/ul>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"768\" src=\"https:\/\/depts.washington.edu\/keck\/wordpress\/wp-content\/uploads\/2021\/12\/IMG_6067-1024x768.jpg\" alt=\"\" class=\"wp-image-818\" srcset=\"https:\/\/depts.washington.edu\/keck\/wordpress\/wp-content\/uploads\/2021\/12\/IMG_6067-1024x768.jpg 1024w, https:\/\/depts.washington.edu\/keck\/wordpress\/wp-content\/uploads\/2021\/12\/IMG_6067-300x225.jpg 300w, https:\/\/depts.washington.edu\/keck\/wordpress\/wp-content\/uploads\/2021\/12\/IMG_6067-768x576.jpg 768w, https:\/\/depts.washington.edu\/keck\/wordpress\/wp-content\/uploads\/2021\/12\/IMG_6067-1536x1152.jpg 1536w, https:\/\/depts.washington.edu\/keck\/wordpress\/wp-content\/uploads\/2021\/12\/IMG_6067-1600x1200.jpg 1600w, https:\/\/depts.washington.edu\/keck\/wordpress\/wp-content\/uploads\/2021\/12\/IMG_6067.jpg 1800w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<p><\/p>\n\n\n\n<h4 class=\"wp-block-heading\">Nikon Upright Widefield<\/h4>\n\n\n\n<h4 class=\"wp-block-heading\">(currently a backup system, inquire for access)<\/h4>\n\n\n\n<ul class=\"wp-block-list\"><li>Nikon Eclipse E600 upright microscope<\/li><li>Manual Stage<\/li><li>1X, 2X, 4X, 10X, 20X, and 40X dry objectives<\/li><li>63X Oil objective<\/li><li>Blue, green, and red filter cubes <\/li><li>DIC optics<\/li><li>Q-Imaging Retigia EX CCD Camera (monochrome) with optional color filter for imaging histology slides <\/li><li>Q-Capture software <\/li><\/ul>\n\n\n\n<figure class=\"wp-block-image\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"768\" src=\"https:\/\/depts.washington.edu\/keck\/wordpress\/wp-content\/uploads\/2019\/10\/Nikon-Upright-Widefield-1024x768.jpg\" alt=\"\" class=\"wp-image-412\" srcset=\"https:\/\/depts.washington.edu\/keck\/wordpress\/wp-content\/uploads\/2019\/10\/Nikon-Upright-Widefield-1024x768.jpg 1024w, https:\/\/depts.washington.edu\/keck\/wordpress\/wp-content\/uploads\/2019\/10\/Nikon-Upright-Widefield-300x225.jpg 300w, https:\/\/depts.washington.edu\/keck\/wordpress\/wp-content\/uploads\/2019\/10\/Nikon-Upright-Widefield-768x576.jpg 768w, https:\/\/depts.washington.edu\/keck\/wordpress\/wp-content\/uploads\/2019\/10\/Nikon-Upright-Widefield-1600x1200.jpg 1600w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n","protected":false},"excerpt":{"rendered":"<p>Widefield Microscopes The Keck Center&#8217;s widefield microscope systems use fluorescent lamp excitation, filter cubes, and CCD monochrome and\/or RGB-color cameras&hellip;<\/p>\n","protected":false},"author":4,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-258","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/depts.washington.edu\/keck\/wp-json\/wp\/v2\/pages\/258","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/depts.washington.edu\/keck\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/depts.washington.edu\/keck\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/keck\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/keck\/wp-json\/wp\/v2\/comments?post=258"}],"version-history":[{"count":20,"href":"https:\/\/depts.washington.edu\/keck\/wp-json\/wp\/v2\/pages\/258\/revisions"}],"predecessor-version":[{"id":897,"href":"https:\/\/depts.washington.edu\/keck\/wp-json\/wp\/v2\/pages\/258\/revisions\/897"}],"wp:attachment":[{"href":"https:\/\/depts.washington.edu\/keck\/wp-json\/wp\/v2\/media?parent=258"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}