{"id":261,"date":"2019-10-21T23:04:24","date_gmt":"2019-10-21T23:04:24","guid":{"rendered":"http:\/\/depts.washington.edu\/keck\/wordpress\/?page_id=261"},"modified":"2019-10-31T04:24:58","modified_gmt":"2019-10-31T04:24:58","slug":"environmental-control","status":"publish","type":"page","link":"https:\/\/depts.washington.edu\/keck\/environmental-control\/","title":{"rendered":"Environmental Control"},"content":{"rendered":"\n<h3 class=\"wp-block-heading\">Environmental Control Equipment<\/h3>\n\n\n\n<p>The Keck Center has 2 stage-top environmental control chambers for maintaining temperature and\/or atmospheric conditions during live-imaging experiments.  They can accept 35mm glass-bottomed culture dishes and chamber coverslips.  The Keck Center maintains a collection of imaging dishes, chambers, and plates to help researchers plan their experiments.<\/p>\n\n\n\n<h4 class=\"wp-block-heading\"><strong>Imaging Chamber Samples: ibidi, MatTek, Grenier, lumox<\/strong><\/h4>\n\n\n\n<figure class=\"wp-block-image\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"768\" src=\"https:\/\/depts.washington.edu\/keck\/wordpress\/wp-content\/uploads\/2019\/10\/Sample-chambers-1024x768.jpg\" alt=\"\" class=\"wp-image-415\" srcset=\"https:\/\/depts.washington.edu\/keck\/wordpress\/wp-content\/uploads\/2019\/10\/Sample-chambers-1024x768.jpg 1024w, https:\/\/depts.washington.edu\/keck\/wordpress\/wp-content\/uploads\/2019\/10\/Sample-chambers-300x225.jpg 300w, https:\/\/depts.washington.edu\/keck\/wordpress\/wp-content\/uploads\/2019\/10\/Sample-chambers-768x576.jpg 768w, https:\/\/depts.washington.edu\/keck\/wordpress\/wp-content\/uploads\/2019\/10\/Sample-chambers-1600x1200.jpg 1600w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<h4 class=\"wp-block-heading\"><strong>Warner QE-1HC Heated\/Cooled 35mm dish Platform with LCS-1 Liquid Cooling System<\/strong><\/h4>\n\n\n\n<p>This open, perfusion-capable, system accepts 35 mm coverglass-bottomed dishes and is capable of heating, cooling, and maintaining temperature in living, aqueous samples.<\/p>\n\n\n\n<figure class=\"wp-block-image\"><img loading=\"lazy\" decoding=\"async\" width=\"768\" height=\"1024\" src=\"https:\/\/depts.washington.edu\/keck\/wordpress\/wp-content\/uploads\/2019\/10\/Warner-Temp-Controller-768x1024.jpg\" alt=\"\" class=\"wp-image-416\" srcset=\"https:\/\/depts.washington.edu\/keck\/wordpress\/wp-content\/uploads\/2019\/10\/Warner-Temp-Controller-768x1024.jpg 768w, https:\/\/depts.washington.edu\/keck\/wordpress\/wp-content\/uploads\/2019\/10\/Warner-Temp-Controller-225x300.jpg 225w, https:\/\/depts.washington.edu\/keck\/wordpress\/wp-content\/uploads\/2019\/10\/Warner-Temp-Controller-1600x2133.jpg 1600w\" sizes=\"auto, (max-width: 768px) 100vw, 768px\" \/><\/figure>\n\n\n\n<h4 class=\"wp-block-heading\"><strong>Pecon Heatable Mounting Frame KH-R 2000 with optional CO2 Cover KH &amp; Heated Humidifier<\/strong><\/h4>\n\n\n\n<p>This system can be used in an open configuration for temperature control only, or in a closed configuration with an optional gas-fed lid for maintaining a humidified, 5% CO2 atmosphere to maintain physiological pH during longer live-imaging experiments.  It can accept both 35mm coverglass-bottomed dishes and chamber coverslips.<\/p>\n\n\n\n<figure class=\"wp-block-image\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"768\" src=\"https:\/\/depts.washington.edu\/keck\/wordpress\/wp-content\/uploads\/2019\/10\/Pecon-Stage-Incubator-1024x768.jpg\" alt=\"\" class=\"wp-image-417\" srcset=\"https:\/\/depts.washington.edu\/keck\/wordpress\/wp-content\/uploads\/2019\/10\/Pecon-Stage-Incubator-1024x768.jpg 1024w, https:\/\/depts.washington.edu\/keck\/wordpress\/wp-content\/uploads\/2019\/10\/Pecon-Stage-Incubator-300x225.jpg 300w, https:\/\/depts.washington.edu\/keck\/wordpress\/wp-content\/uploads\/2019\/10\/Pecon-Stage-Incubator-768x576.jpg 768w, https:\/\/depts.washington.edu\/keck\/wordpress\/wp-content\/uploads\/2019\/10\/Pecon-Stage-Incubator-1600x1200.jpg 1600w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<figure class=\"wp-block-image\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"768\" src=\"https:\/\/depts.washington.edu\/keck\/wordpress\/wp-content\/uploads\/2019\/10\/Pecon-Stage-Incubator-2-1024x768.jpg\" alt=\"\" class=\"wp-image-413\" srcset=\"https:\/\/depts.washington.edu\/keck\/wordpress\/wp-content\/uploads\/2019\/10\/Pecon-Stage-Incubator-2-1024x768.jpg 1024w, https:\/\/depts.washington.edu\/keck\/wordpress\/wp-content\/uploads\/2019\/10\/Pecon-Stage-Incubator-2-300x225.jpg 300w, https:\/\/depts.washington.edu\/keck\/wordpress\/wp-content\/uploads\/2019\/10\/Pecon-Stage-Incubator-2-768x576.jpg 768w, https:\/\/depts.washington.edu\/keck\/wordpress\/wp-content\/uploads\/2019\/10\/Pecon-Stage-Incubator-2-1600x1200.jpg 1600w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n","protected":false},"excerpt":{"rendered":"<p>Environmental Control Equipment The Keck Center has 2 stage-top environmental control chambers for maintaining temperature and\/or atmospheric conditions during live-imaging&hellip;<\/p>\n","protected":false},"author":4,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-261","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/depts.washington.edu\/keck\/wp-json\/wp\/v2\/pages\/261","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/depts.washington.edu\/keck\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/depts.washington.edu\/keck\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/keck\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/keck\/wp-json\/wp\/v2\/comments?post=261"}],"version-history":[{"count":9,"href":"https:\/\/depts.washington.edu\/keck\/wp-json\/wp\/v2\/pages\/261\/revisions"}],"predecessor-version":[{"id":502,"href":"https:\/\/depts.washington.edu\/keck\/wp-json\/wp\/v2\/pages\/261\/revisions\/502"}],"wp:attachment":[{"href":"https:\/\/depts.washington.edu\/keck\/wp-json\/wp\/v2\/media?parent=261"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}