{"id":2737,"date":"2019-03-19T19:14:36","date_gmt":"2019-03-19T19:14:36","guid":{"rendered":"http:\/\/depts.washington.edu\/kellylab\/?page_id=2737"},"modified":"2019-03-19T19:14:39","modified_gmt":"2019-03-19T19:14:39","slug":"protocols","status":"publish","type":"page","link":"https:\/\/depts.washington.edu\/kellylab\/protocols\/","title":{"rendered":"Protocols"},"content":{"rendered":"<section class=\"kc-elm kc-css-60687 kc_row\"><div class=\"kc-row-container  kc-container\"><div class=\"kc-wrap-columns\"><div class=\"kc-elm kc-css-964358 kc_col-sm-12 kc_column kc_col-sm-12\"><div class=\"kc-col-container\"><div class=\"kc-elm kc-css-219441 kc_text_block\"><div>\u00a0<\/div>\n<div>\u00a0<\/div>\n<div>\u00a0<\/div>\n<div class=\"wp-block-file\"><a href=\"http:\/\/depts.washington.edu\/kellylab\/wordpress\/wp-content\/uploads\/2021\/12\/qRT-PCR-SOP.doc\">SOP for Generating cDNA from Total RNA for RTPCR<\/a><a class=\"wp-block-file__button\" href=\"http:\/\/depts.washington.edu\/kellylab\/wordpress\/wp-content\/uploads\/2021\/12\/qRT-PCR-SOP.doc\" download=\"\">Download<\/a><\/div>\n<p><!-- \/wp:file --><\/p>\n<div class=\"wp-block-file\"><a href=\"http:\/\/depts.washington.edu\/kellylab\/wordpress\/wp-content\/uploads\/2019\/03\/ECFC_SOP.doc\">SOP for Endothelial Colony Forming Cells (ECFCs), also known as Endothelial Progenitor Cells (EPCs)<\/a><a class=\"wp-block-file__button\" href=\"http:\/\/depts.washington.edu\/kellylab\/wordpress\/wp-content\/uploads\/2019\/03\/ECFC_SOP.doc\" download=\"\">Download<\/a><\/div>\n<p><!-- \/wp:file --><\/p>\n<p><!-- wp:file {\"id\":2739,\"href\":\"http:\/\/depts.washington.edu\/kellylab\/wordpress\/wp-content\/uploads\/2019\/03\/ICC-Materials-and-Methods.doc\"} --><\/p>\n<div class=\"wp-block-file\"><a href=\"http:\/\/depts.washington.edu\/kellylab\/wordpress\/wp-content\/uploads\/2019\/03\/ICC-Materials-and-Methods.doc\">Immunocytochemistry (ICC) Materials and Methods<\/a><a class=\"wp-block-file__button\" href=\"http:\/\/depts.washington.edu\/kellylab\/wordpress\/wp-content\/uploads\/2019\/03\/ICC-Materials-and-Methods.doc\" download=\"\">Download<\/a><\/div>\n<p><!-- \/wp:file --><\/p>\n<p><!-- wp:file {\"id\":2740,\"href\":\"http:\/\/depts.washington.edu\/kellylab\/wordpress\/wp-content\/uploads\/2019\/03\/QIAGEN_protocol.pdf\"} --><\/p>\n<div class=\"wp-block-file\"><a href=\"http:\/\/depts.washington.edu\/kellylab\/wordpress\/wp-content\/uploads\/2019\/03\/QIAGEN_protocol.pdf\">QIAGEN DNA Purification<\/a><a class=\"wp-block-file__button\" href=\"http:\/\/depts.washington.edu\/kellylab\/wordpress\/wp-content\/uploads\/2019\/03\/QIAGEN_protocol.pdf\" download=\"\">Download<\/a><\/div>\n<p><!-- \/wp:file --><\/p>\n<p><!-- wp:file {\"id\":2741,\"href\":\"http:\/\/depts.washington.edu\/kellylab\/wordpress\/wp-content\/uploads\/2019\/03\/Trypan-Blue-Exclusion-Protocol.docx\"} --><\/p>\n<div class=\"wp-block-file\"><a href=\"http:\/\/depts.washington.edu\/kellylab\/wordpress\/wp-content\/uploads\/2019\/03\/Trypan-Blue-Exclusion-Protocol.docx\">Trypan Blue Exclusion<\/a><a class=\"wp-block-file__button\" href=\"http:\/\/depts.washington.edu\/kellylab\/wordpress\/wp-content\/uploads\/2019\/03\/Trypan-Blue-Exclusion-Protocol.docx\" download=\"\">Download<\/a><\/div>\n<p><!-- \/wp:file --><\/p>\n<p><!-- wp:file {\"id\":2741,\"href\":\"http:\/\/depts.washington.edu\/kellylab\/wordpress\/wp-content\/uploads\/2020\/10\/Genotyping-Cre-Mice-Protocol.docx\"} --><\/p>\n<div class=\"wp-block-file\"><a href=\"http:\/\/depts.washington.edu\/kellylab\/wordpress\/wp-content\/uploads\/2020\/10\/Genotyping-Cre-Mice-Protocol.docx\">Mice Genotyping<\/a><a class=\"wp-block-file__button\" href=\"http:\/\/depts.washington.edu\/kellylab\/wordpress\/wp-content\/uploads\/2020\/10\/Genotyping-Cre-Mice-Protocol.docx\" download=\"\">Download<\/a><\/div>\n<p><!-- \/wp:file --><\/p>\n<p><!-- wp:file {\"id\":2738,\"href\":\"http:\/\/depts.washington.edu\/kellylab\/wordpress\/wp-content\/uploads\/2020\/10\/Silver-Stain-Protocol.docx\"} --><\/p>\n<div class=\"wp-block-file\"><a href=\"http:\/\/depts.washington.edu\/kellylab\/wordpress\/wp-content\/uploads\/2020\/10\/Silver-Stain-Protocol.docx\">Silver Stain<\/a><a class=\"wp-block-file__button\" href=\"http:\/\/depts.washington.edu\/kellylab\/wordpress\/wp-content\/uploads\/2020\/10\/Silver-Stain-Protocol.docx\" download=\"\">Download<\/a><\/div>\n<p><!-- \/wp:file --><\/p>\n<p><!-- wp:file {\"id\":2738,\"href\":http:\/\/depts.washington.edu\/kellylab\/wordpress\/wp-content\/uploads\/2020\/10\/Tri-Reagent-Protocol.pdf\"} --><\/p>\n<div class=\"wp-block-file\"><a href=\"http:\/\/depts.washington.edu\/kellylab\/wordpress\/wp-content\/uploads\/2020\/10\/Tri-Reagent-Protocol.pdf\">Tri Reagent Protocol<\/a><a class=\"wp-block-file__button\" href=\"http:\/\/depts.washington.edu\/kellylab\/wordpress\/wp-content\/uploads\/2020\/10\/Tri-Reagent-Protocol.pdf\" download=\"\">Download<\/a><\/div>\n<p><!-- \/wp:file --><\/p>\n<p><!-- wp:file {\"id\":2738,\"href\":http:\/\/depts.washington.edu\/kellylab\/wordpress\/wp-content\/uploads\/2020\/10\/Hazardous-Waste-Disposal-kt.doc\"} --><\/p>\n<div class=\"wp-block-file\"><a href=\"http:\/\/depts.washington.edu\/kellylab\/wordpress\/wp-content\/uploads\/2020\/10\/Hazardous-Waste-Disposal-kt.doc\">Hazardous Waste Disposal<\/a><a class=\"wp-block-file__button\" href=\"http:\/\/depts.washington.edu\/kellylab\/wordpress\/wp-content\/uploads\/2020\/10\/Hazardous-Waste-Disposal-kt.doc\" download=\"\">Download<\/a><\/div>\n<p><!-- \/wp:file --><\/p>\n<p><!-- wp:file {\"id\":2738,\"href\":http:\/\/depts.washington.edu\/kellylab\/wordpress\/wp-content\/uploads\/2020\/10\/Kelly-Lab-SOP-for-Acrylamide-Use.docx\"} --><\/p>\n<div class=\"wp-block-file\"><a href=\"http:\/\/depts.washington.edu\/kellylab\/wordpress\/wp-content\/uploads\/2020\/10\/Kelly-Lab-SOP-for-Acrylamide-Use.docx\">Kelly Lab SOP for Acrylamide Use<\/a><a class=\"wp-block-file__button\" href=\"http:\/\/depts.washington.edu\/kellylab\/wordpress\/wp-content\/uploads\/2020\/10\/Kelly-Lab-SOP-for-Acrylamide-Use.docx\" download=\"\">Download<\/a><\/div>\n<p><!-- \/wp:file --><\/p>\n<p><!-- wp:file {\"id\":2738,\"href\":http:\/\/depts.washington.edu\/kellylab\/wordpress\/wp-content\/uploads\/2020\/10\/Kelly-Lab-SOP-for-Flammable-Solvents-Use.docx\"} --><\/p>\n<div class=\"wp-block-file\"><a href=\"http:\/\/depts.washington.edu\/kellylab\/wordpress\/wp-content\/uploads\/2020\/10\/Kelly-Lab-SOP-for-Flammable-Solvents-Use.docx\">Kelly Lab SOP for Flammable Solvents<\/a><a class=\"wp-block-file__button\" href=\"http:\/\/depts.washington.edu\/kellylab\/wordpress\/wp-content\/uploads\/2020\/10\/Kelly-Lab-SOP-for-Flammable-Solvents-Use.docx\" download=\"\">Download<\/a><\/div>\n<p><!-- \/wp:file --><\/p>\n<p><!-- wp:file {\"id\":2738,\"href\":http:\/\/depts.washington.edu\/kellylab\/wordpress\/wp-content\/uploads\/2020\/10\/Whole-Mount-X-gal.docx\"} --><\/p>\n<div class=\"wp-block-file\"><a href=\"http:\/\/depts.washington.edu\/kellylab\/wordpress\/wp-content\/uploads\/2020\/10\/Whole-Mount-X-gal.docx\">Whole Mount X-gal Histochemistry of Transgenic Animal<\/a><a class=\"wp-block-file__button\" href=\"http:\/\/depts.washington.edu\/kellylab\/wordpress\/wp-content\/uploads\/2020\/10\/Whole-Mount-X-gal.docx\" download=\"\">Download<\/a><\/div>\n<p><!-- \/wp:file --><\/p>\n<p><!-- wp:file {\"id\":2738,\"href\":http:\/\/depts.washington.edu\/kellylab\/wordpress\/wp-content\/uploads\/2020\/10\/Radiolabeled-Digoxin-Stock-SOP.doc\"} --><\/p>\n<div class=\"wp-block-file\"><a href=\"http:\/\/depts.washington.edu\/kellylab\/wordpress\/wp-content\/uploads\/2020\/10\/Radiolabeled-Digoxin-Stock-SOP.doc\">Radiolabeled Digoxin Stock SOP<\/a><a class=\"wp-block-file__button\" href=\"http:\/\/depts.washington.edu\/kellylab\/wordpress\/wp-content\/uploads\/2020\/10\/Radiolabeled-Digoxin-Stock-SOP.doc\" download=\"\">Download<\/a><\/div>\n<p><!-- \/wp:file --><\/p>\n<p><!-- wp:file {\"id\":2738,\"href\":http:\/\/depts.washington.edu\/kellylab\/wordpress\/wp-content\/uploads\/2020\/10\/RAM-Lab-Practices_SOP.doc\"} --><\/p>\n<div class=\"wp-block-file\"><a href=\"http:\/\/depts.washington.edu\/kellylab\/wordpress\/wp-content\/uploads\/2020\/10\/RAM-Lab-Practices_SOP.doc\">Kelly Lab Practices for Working with Radioactive Materials<\/a><a class=\"wp-block-file__button\" href=\"http:\/\/depts.washington.edu\/kellylab\/wordpress\/wp-content\/uploads\/2020\/10\/RAM-Lab-Practices_SOP.doc\" download=\"\">Download<\/a><\/div>\n<p><!-- \/wp:file --><\/p>\n<p><!-- wp:file {\"id\":2738,\"href\":http:\/\/depts.washington.edu\/kellylab\/wordpress\/wp-content\/uploads\/2020\/10\/RAM-Waste-Lab-Practices_SOP.doc\"} --><\/p>\n<div class=\"wp-block-file\"><a href=\"http:\/\/depts.washington.edu\/kellylab\/wordpress\/wp-content\/uploads\/2020\/10\/RAM-Waste-Lab-Practices_SOP.doc\">Kelly Lab Practices for Disposing Radioactive Waste<\/a><a class=\"wp-block-file__button\" href=\"http:\/\/depts.washington.edu\/kellylab\/wordpress\/wp-content\/uploads\/2020\/10\/RAM-Waste-Lab-Practices_SOP.doc\" download=\"\">Download<\/a><\/div>\n<p><!-- \/wp:file --><\/p>\n<p><!-- wp:file {\"id\":2738,\"href\":http:\/\/depts.washington.edu\/kellylab\/wordpress\/wp-content\/uploads\/2020\/10\/Carcinogens_SOP.doc\"} --><\/p>\n<div class=\"wp-block-file\"><a href=\"http:\/\/depts.washington.edu\/kellylab\/wordpress\/wp-content\/uploads\/2020\/10\/Carcinogens_SOP.doc\">Carcinogens<\/a><a class=\"wp-block-file__button\" href=\"http:\/\/depts.washington.edu\/kellylab\/wordpress\/wp-content\/uploads\/2020\/10\/Carcinogens_SOP.doc\" download=\"\">Download<\/a><\/div>\n<p><!-- \/wp:file --><\/p>\n<p><!-- wp:file {\"id\":2738,\"href\":http:\/\/depts.washington.edu\/kellylab\/wordpress\/wp-content\/uploads\/2020\/10\/Ethidium-Bromide.doc\"} --><\/p>\n<div class=\"wp-block-file\"><a href=\"http:\/\/depts.washington.edu\/kellylab\/wordpress\/wp-content\/uploads\/2020\/10\/Ethidium-Bromide.doc\">Ethidium Bromide<\/a><a class=\"wp-block-file__button\" href=\"http:\/\/depts.washington.edu\/kellylab\/wordpress\/wp-content\/uploads\/2020\/10\/Ethidium-Bromide.doc\" download=\"\">Download<\/a><\/div>\n<p><!-- \/wp:file --><\/p>\n<p><!-- wp:file {\"id\":2738,\"href\":http:\/\/depts.washington.edu\/kellylab\/wordpress\/wp-content\/uploads\/2020\/10\/EdsMiniPrep.docx\"} --><\/p>\n<div class=\"wp-block-file\"><a href=\"http:\/\/depts.washington.edu\/kellylab\/wordpress\/wp-content\/uploads\/2020\/10\/EdsMiniPrep.docx\">Alkaline Lysis MiniPrep<\/a><a class=\"wp-block-file__button\" href=\"http:\/\/depts.washington.edu\/kellylab\/wordpress\/wp-content\/uploads\/2020\/10\/EdsMiniPrep.docx\" download=\"\">Download<\/a><\/div>\n<p><!-- \/wp:file --><\/p>\n<div>\u00a0<\/div>\n<div>\u00a0<\/div>\n<\/div><\/div><\/div><\/div><\/div><\/section>","protected":false},"excerpt":{"rendered":"","protected":false},"author":3,"featured_media":2288,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/depts.washington.edu\/kellylab\/wp-json\/wp\/v2\/pages\/2737"}],"collection":[{"href":"https:\/\/depts.washington.edu\/kellylab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/depts.washington.edu\/kellylab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/kellylab\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/kellylab\/wp-json\/wp\/v2\/comments?post=2737"}],"version-history":[{"count":5,"href":"https:\/\/depts.washington.edu\/kellylab\/wp-json\/wp\/v2\/pages\/2737\/revisions"}],"predecessor-version":[{"id":2878,"href":"https:\/\/depts.washington.edu\/kellylab\/wp-json\/wp\/v2\/pages\/2737\/revisions\/2878"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/kellylab\/wp-json\/wp\/v2\/media\/2288"}],"wp:attachment":[{"href":"https:\/\/depts.washington.edu\/kellylab\/wp-json\/wp\/v2\/media?parent=2737"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}