14 Tesla (600 MHz) High Resolution IMaging (HRIM)

The High Resolution Nuclear Magnetic Resonance (NMR) Spectroscopy, Magnetic Resonance Imaging (MRI), and Magnetic Resonance Spectroscopy (MRS) Core Facility at the South Lake Union campus assists researchers from the University of Washington Mitochondria and Metabolism Center and the research community at large.

The facility is capable of multi-nuclear, high resolution NMR as well as in vivo MRS and micro MRI.  All projects are welcome.


The cornerstone of the facility is a state-of-the-art 14 Tesla (600 MHz) vertical wide bore Bruker Magnetic Resonance Spectrometer. The magnet system is fully equipped for NMR and provides cost effective in vivo MRI and MRS in small animals, such as mice.

It is equipped with a sample temperature control unit and a biological sensor system directly linked to the instrument, allowing animal monitoring and control of studies.  The laboratory has a satellite workstation for data processing, a lab bench area for sample/animal preparation, an isoflurane anesthesia system for  in-vivo work, and a small refrigerator for temporary storage of samples and reagents.

The facility is currently approved for non-survival and survival work at the BSL-1 and BSL-2 levels and can be added to researchers’ protocols at these levels. Please review our Lab Safety Resources page to find links to the UW IACUC and their required forms.

Equipment and Software

High Resolution Spectroscopy

Software: TOPSPIN (Bruker-Biospin)


High Resolution Probes
Type Tuning Range Variable Temperature Range
3 mm BBO 15N – 31P with 1H decoupling -120 to 150 °C
5 mm BBO 15N to 31P with 1H decoupling -150 to 180 °C
10 mm BBO 109Ag to 31P with 1H decoupling -130 to 150 °C
20 mm BBO 109Ag to 31P with 1H decoupling



Software: ParaVision (Bruker-Biospin)

Micro-AHS/RF2.5 In Vivo 25 mm ID micro-imaging probe with:
  • Two volume coil inserts: 1H and 1H/23Na dual tuned
  • A 1H/31P surface coil insert
MicroMouse 2.5-In Vivo 30 mm ID micro-imaging probe
  • A 1H-19F/31P volume coil