Below you will find links to tutorials about the IMAGEGUI, the main program for MVA of images in the NB toolbox. I have taken a lot of time creating step by step tutorials on how to use each function within the IMAGEGUI.<\/p>\n\n\n\n
The tutorials are available as pdf downloads. In each case the tutorials will take you step by step through how to use the functions of the gui.<\/p>\n\n\n\n
For best performance the IMAGEGUI should be used on a high end PC<\/strong>. The more memory and faster CPU the better.<\/strong> The IMAGEGUI will cause Matlab to run out of memory if you import image data sets with a large number of peak images (>100 to 150 peaks). I am working on improving the performance, but this is not easy with Matlab. These tutorials were created from screen shots that were 1034×934 pixels. They should work fine on any modern computer monitor. You can download all of the pdf tutorials in a zip file or view\/save each of them individually. To view a pdf tutorial click on the link. To save, right-click and choose ‘save as’.<\/p>\n\n\n\n If you are going to use the standalone version please see this tutorial NBToolbox Standalone – Important Information<\/a><\/p>\n\n\n\n Download all imagegui pdf tutorials in a zip file<\/a><\/p>\n\n\n\n 1. General Introduction<\/a><\/p>\n\n\n\n 2. Importing Data<\/a><\/p>\n\n\n\n 3. Normalizing Image Data<\/a><\/p>\n\n\n\n 4. Cropping an Image<\/a><\/p>\n\n\n\n 5. Filtering an Image<\/a><\/p>\n\n\n\n 6. Image PCA<\/a><\/p>\n\n\n\n 7. Plotting Image Scores<\/a><\/p>\n\n\n\n 8. Exporting MVA data<\/a><\/p>\n\n\n\n 9. Plotting Image Data<\/a><\/p>\n\n\n\n 10. Making RGB Overlays<\/a><\/p>\n\n\n\n 11. Making Scalebars on Images<\/a><\/p>\n\n\n\n 12. Using the Image Properties Panel<\/a><\/p>\n\n\n\n 13. Dice and Classify Image Function<\/a><\/p>\n\n\n\n 14. Create Image Ratio Plot<\/a><\/p>\n\n\n\n 15. One Way to Import From PHI RAW data<\/a><\/p>\n\n\n\n 16. Creating and Labeling Loadings Plots<\/a><\/p>\n\n\n\n 17. Cut Up Stage Raster<\/a><\/p>\n\n\n\n 18. Align Images<\/a><\/p>\n\n\n\n 19. Extract ROI<\/a><\/p>\n\n\n\n 20. MCR<\/a><\/p>\n\n\n\n 21. PC Data Browser<\/a><\/p>\n\n\n\n 22. Plot Pseudo Spectrum<\/a><\/p>\n\n\n\n 23. Image Processing<\/a><\/p>\n\n\n\n 24. Import imzML Data<\/a><\/p>\n\n\n\n 25. Correlated Image Finder<\/a><\/p>\n\n\n\n 26. Auto ROI Extractor<\/a><\/p>\n\n\n\n 27. K Means<\/a><\/p>\n\n\n\n 28. MSF Filtering<\/a><\/p>\n\n\n\n <\/p>\n","protected":false},"excerpt":{"rendered":" Below you will find links to tutorials about the IMAGEGUI, the main program for MVA of images in the NB toolbox. I have taken a lot of time creating step by step tutorials on how to use each function within the IMAGEGUI. You must go through ALL tutorials in order to use the IMAGEGUI properly.Continue reading “Imagegui Tutorials Page”<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-604","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/depts.washington.edu\/nesacbio\/wp-json\/wp\/v2\/pages\/604","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/depts.washington.edu\/nesacbio\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/depts.washington.edu\/nesacbio\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/nesacbio\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/nesacbio\/wp-json\/wp\/v2\/comments?post=604"}],"version-history":[{"count":3,"href":"https:\/\/depts.washington.edu\/nesacbio\/wp-json\/wp\/v2\/pages\/604\/revisions"}],"predecessor-version":[{"id":637,"href":"https:\/\/depts.washington.edu\/nesacbio\/wp-json\/wp\/v2\/pages\/604\/revisions\/637"}],"wp:attachment":[{"href":"https:\/\/depts.washington.edu\/nesacbio\/wp-json\/wp\/v2\/media?parent=604"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
If you run into ‘Out of Memory’ errors, try reducing the size of the data set by down binning the image, or reducing the number of peak images in the data set.
You must include the total counts image and sum of rest image when exporting .bif\/.bif6\/.bif3d files from the Iontof software.<\/strong><\/p>\n\n\n\n