{"id":1669,"date":"2020-10-20T09:44:25","date_gmt":"2020-10-20T16:44:25","guid":{"rendered":"https:\/\/depts.washington.edu\/pandemicalliance\/?p=1669"},"modified":"2020-10-21T09:53:28","modified_gmt":"2020-10-21T16:53:28","slug":"pooling-of-samples-to-optimize-sars-cov-2-diagnosis-by-rt-qpcr-comparative-analysis-of-two-protocols","status":"publish","type":"post","link":"https:\/\/depts.washington.edu\/pandemicalliance\/2020\/10\/20\/pooling-of-samples-to-optimize-sars-cov-2-diagnosis-by-rt-qpcr-comparative-analysis-of-two-protocols\/","title":{"rendered":"Pooling of Samples to Optimize SARS-CoV-2 Diagnosis by RT-QPCR: Comparative Analysis of Two Protocols"},"content":{"rendered":"<ul>\n<li>Pooling naso- or oropharyngeal swab samples prior to RNA extraction was associated with 100% diagnostic accuracy, while pooling after individual-sample RNA extraction was associated with 91% diagnostic accuracy. Volpato et al. conclude that testing protocols should call for pooling swab samples before RNA extraction.<\/li>\n<\/ul>\n<p>Volpato et al. (Oct 16, 2020). Pooling of Samples to Optimize SARS-CoV-2 Diagnosis by RT-QPCR: Comparative Analysis of Two Protocols. European Journal of Clinical Microbiology &amp; Infectious Diseases. <a href=\"https:\/\/doi.org\/10.1007\/s10096-020-04071-8\">https:\/\/doi.org\/10.1007\/s10096-020-04071-8<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Pooling naso- or oropharyngeal swab samples prior to RNA extraction was associated with 100% diagnostic accuracy, while pooling after individual-sample RNA extraction was associated with 91% diagnostic accuracy. Volpato et al. conclude that testing protocols should call for pooling swab samples before RNA extraction. Volpato et al. (Oct 16, 2020). Pooling of Samples to Optimize&#8230;<\/p>\n<div><a class=\"more\" href=\"https:\/\/depts.washington.edu\/pandemicalliance\/2020\/10\/20\/pooling-of-samples-to-optimize-sars-cov-2-diagnosis-by-rt-qpcr-comparative-analysis-of-two-protocols\/\">Read more<\/a><\/div>\n","protected":false},"author":5,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":"","_links_to":"","_links_to_target":""},"categories":[6],"tags":[27],"topic":[19],"class_list":["post-1669","post","type-post","status-publish","format-standard","hentry","category-article-summary","tag-testing","topic-testing-and-treatment"],"_links":{"self":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/1669","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/users\/5"}],"replies":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/comments?post=1669"}],"version-history":[{"count":1,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/1669\/revisions"}],"predecessor-version":[{"id":1670,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/1669\/revisions\/1670"}],"wp:attachment":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/media?parent=1669"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/categories?post=1669"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/tags?post=1669"},{"taxonomy":"topic","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/topic?post=1669"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}