{"id":2157,"date":"2020-11-13T11:02:16","date_gmt":"2020-11-13T19:02:16","guid":{"rendered":"https:\/\/depts.washington.edu\/pandemicalliance\/?p=2157"},"modified":"2020-11-16T11:03:22","modified_gmt":"2020-11-16T19:03:22","slug":"validation-and-testing-of-a-method-for-detection-of-sars-cov-2-rna-in-healthy-human-stool","status":"publish","type":"post","link":"https:\/\/depts.washington.edu\/pandemicalliance\/2020\/11\/13\/validation-and-testing-of-a-method-for-detection-of-sars-cov-2-rna-in-healthy-human-stool\/","title":{"rendered":"Validation and Testing of a Method for Detection of SARS-CoV-2 RNA in Healthy Human Stool"},"content":{"rendered":"<ul>\n<li>[Pre-print, not peer-reviewed] An assay designed to detect SARS-CoV-2 RNA in stool based upon a modified version of the CDC rRT-PCR SARS-CoV-2 test. Using stool samples from health individuals spiked with inactivated SAR-CoV-2 virus, the lower limit of detection (LoD) of the assay was found to be 3,000 viral RNA copies per gram of original stool sample. Thirty contrived SARS-CoV-2 samples were tested by a second laboratory and were correctly identified as positive or negative in at least one of two rounds of testing. Samples from individuals with COVID-19 were also tested, and the assay was able to detect SARS-CoV-2 RNA in 3 of the 5 samples.<\/li>\n<\/ul>\n<p>Coryell et al. (Nov 12, 2020). Validation and Testing of a Method for Detection of SARS-CoV-2 RNA in Healthy Human Stool. MedRxiv. <a href=\"https:\/\/doi.org\/10.1101\/2020.11.09.20228601\">https:\/\/doi.org\/10.1101\/2020.11.09.20228601<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>[Pre-print, not peer-reviewed] An assay designed to detect SARS-CoV-2 RNA in stool based upon a modified version of the CDC rRT-PCR SARS-CoV-2 test. Using stool samples from health individuals spiked with inactivated SAR-CoV-2 virus, the lower limit of detection (LoD) of the assay was found to be 3,000 viral RNA copies per gram of original&#8230;<\/p>\n<div><a class=\"more\" href=\"https:\/\/depts.washington.edu\/pandemicalliance\/2020\/11\/13\/validation-and-testing-of-a-method-for-detection-of-sars-cov-2-rna-in-healthy-human-stool\/\">Read more<\/a><\/div>\n","protected":false},"author":5,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":"","_links_to":"","_links_to_target":""},"categories":[6],"tags":[27],"topic":[19],"class_list":["post-2157","post","type-post","status-publish","format-standard","hentry","category-article-summary","tag-testing","topic-testing-and-treatment"],"_links":{"self":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/2157","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/users\/5"}],"replies":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/comments?post=2157"}],"version-history":[{"count":1,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/2157\/revisions"}],"predecessor-version":[{"id":2158,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/2157\/revisions\/2158"}],"wp:attachment":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/media?parent=2157"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/categories?post=2157"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/tags?post=2157"},{"taxonomy":"topic","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/topic?post=2157"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}