{"id":3543,"date":"2021-01-29T20:04:42","date_gmt":"2021-01-30T04:04:42","guid":{"rendered":"https:\/\/depts.washington.edu\/pandemicalliance\/?p=3543"},"modified":"2021-02-22T22:43:05","modified_gmt":"2021-02-23T06:43:05","slug":"a-rapid-and-low-cost-protocol-for-the-detection-of-b-1-1-7-lineage-of-sars-cov-2-by-using-sybr-green-based-rt-qpcr","status":"publish","type":"post","link":"https:\/\/depts.washington.edu\/pandemicalliance\/2021\/01\/29\/a-rapid-and-low-cost-protocol-for-the-detection-of-b-1-1-7-lineage-of-sars-cov-2-by-using-sybr-green-based-rt-qpcr\/","title":{"rendered":"A Rapid and Low-Cost protocol for the detection of B.1.1.7 lineage of SARS-CoV-2 by using SYBR Green-Based RT-qPCR"},"content":{"rendered":"<p><i>[Pre-print, not peer-reviewed]<\/i> Abdel-Sater et al. reported the development of a rapid molecular test to identify the SARS-CoV-2 B.1.1.7 (UK) variant using a set of RT-PCR primers that were designed to confirm the deletion mutations \u039469\/\u039470 in the spike and the \u0394106\/\u0394107\/\u0394108 in the NSP6 gene. The large-scale screening method may help bypass the need for widespread sequencing to confirm the presence of both the B.1.1.7 variant and variants with similar deletions.<\/p>\n<p><i>Abdel-Sater et al. (Jan 29, 2021). A Rapid and Low-Cost protocol for the detection of B.1.1.7 lineage of SARS-CoV-2 by using SYBR Green-Based RT-qPCR. Pre-print downloaded Jan 29 from <\/i><a href=\"https:\/\/doi.org\/10.1101\/2021.01.27.21250048\">https:\/\/doi.org\/10.1101\/2021.01.27.21250048<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>[Pre-print, not peer-reviewed] Abdel-Sater et al. reported the development of a rapid molecular test to identify the SARS-CoV-2 B.1.1.7 (UK) variant using a set of RT-PCR primers that were designed to confirm the deletion mutations \u039469\/\u039470 in the spike and the \u0394106\/\u0394107\/\u0394108 in the NSP6 gene. The large-scale screening method may help bypass the need&#8230;<\/p>\n<div><a class=\"more\" href=\"https:\/\/depts.washington.edu\/pandemicalliance\/2021\/01\/29\/a-rapid-and-low-cost-protocol-for-the-detection-of-b-1-1-7-lineage-of-sars-cov-2-by-using-sybr-green-based-rt-qpcr\/\">Read more<\/a><\/div>\n","protected":false},"author":7,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":"","_links_to":"","_links_to_target":""},"categories":[6],"tags":[27,159],"topic":[19],"class_list":["post-3543","post","type-post","status-publish","format-standard","hentry","category-article-summary","tag-testing","tag-variants","topic-testing-and-treatment"],"_links":{"self":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/3543","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/users\/7"}],"replies":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/comments?post=3543"}],"version-history":[{"count":1,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/3543\/revisions"}],"predecessor-version":[{"id":3544,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/3543\/revisions\/3544"}],"wp:attachment":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/media?parent=3543"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/categories?post=3543"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/tags?post=3543"},{"taxonomy":"topic","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/topic?post=3543"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}