{"id":3690,"date":"2021-02-04T17:58:51","date_gmt":"2021-02-05T01:58:51","guid":{"rendered":"https:\/\/depts.washington.edu\/pandemicalliance\/?p=3690"},"modified":"2021-02-04T17:58:51","modified_gmt":"2021-02-05T01:58:51","slug":"rapid-sensitive-and-specific-severe-acute-respiratory-syndrome-coronavirus-2-detection-a-multicenter-comparison-between-standard-quantitative-reverse-transcriptase-polymerase-chain-reaction-and-cri","status":"publish","type":"post","link":"https:\/\/depts.washington.edu\/pandemicalliance\/2021\/02\/04\/rapid-sensitive-and-specific-severe-acute-respiratory-syndrome-coronavirus-2-detection-a-multicenter-comparison-between-standard-quantitative-reverse-transcriptase-polymerase-chain-reaction-and-cri\/","title":{"rendered":"Rapid, Sensitive, and Specific Severe Acute Respiratory Syndrome Coronavirus 2 Detection: A Multicenter Comparison Between Standard Quantitative Reverse-Transcriptase Polymerase Chain Reaction and CRISPR-Based DETECTR"},"content":{"rendered":"<p>The DETECTR assay (a combination of RT-LAMP and RT-Cas12-RNP) was found to have similar sensitivity but superior specificity to detect SARS-CoV-2 when compared to qRT-PCR on 378 patient samples derived from routine testing. Since the DETECTR assay is not as susceptible to false negatives from N-gene mutations and not as resource intensive to perform, the authors suggest the assay could complement qRT-PCR to increase testing capacity and improve specificity.<\/p>\n<p><i>Brandsma et al. (Oct 10, 2020). Rapid, Sensitive, and Specific Severe Acute Respiratory Syndrome Coronavirus 2 Detection: A Multicenter Comparison Between Standard Quantitative Reverse-Transcriptase Polymerase Chain Reaction and CRISPR-Based DETECTR. The Journal of Infectious Diseases. <\/i><a href=\"https:\/\/doi.org\/10.1093\/infdis\/jiaa641\">https:\/\/doi.org\/10.1093\/infdis\/jiaa641<\/a><i><span class=\"Apple-converted-space\">\u00a0<\/span><\/i><\/p>\n","protected":false},"excerpt":{"rendered":"<p>The DETECTR assay (a combination of RT-LAMP and RT-Cas12-RNP) was found to have similar sensitivity but superior specificity to detect SARS-CoV-2 when compared to qRT-PCR on 378 patient samples derived from routine testing. Since the DETECTR assay is not as susceptible to false negatives from N-gene mutations and not as resource intensive to perform, the&#8230;<\/p>\n<div><a class=\"more\" href=\"https:\/\/depts.washington.edu\/pandemicalliance\/2021\/02\/04\/rapid-sensitive-and-specific-severe-acute-respiratory-syndrome-coronavirus-2-detection-a-multicenter-comparison-between-standard-quantitative-reverse-transcriptase-polymerase-chain-reaction-and-cri\/\">Read more<\/a><\/div>\n","protected":false},"author":7,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":"","_links_to":"","_links_to_target":""},"categories":[6],"tags":[27],"topic":[19],"class_list":["post-3690","post","type-post","status-publish","format-standard","hentry","category-article-summary","tag-testing","topic-testing-and-treatment"],"_links":{"self":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/3690","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/users\/7"}],"replies":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/comments?post=3690"}],"version-history":[{"count":1,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/3690\/revisions"}],"predecessor-version":[{"id":3691,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/3690\/revisions\/3691"}],"wp:attachment":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/media?parent=3690"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/categories?post=3690"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/tags?post=3690"},{"taxonomy":"topic","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/topic?post=3690"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}