{"id":3766,"date":"2021-02-08T20:15:34","date_gmt":"2021-02-09T04:15:34","guid":{"rendered":"https:\/\/depts.washington.edu\/pandemicalliance\/?p=3766"},"modified":"2021-02-22T22:33:34","modified_gmt":"2021-02-23T06:33:34","slug":"implementation-of-an-in-house-real-time-reverse-transcription-pcr-assay-for-the-rapid-detection-of-the-sars-cov-2-marseille-4-variant","status":"publish","type":"post","link":"https:\/\/depts.washington.edu\/pandemicalliance\/2021\/02\/08\/implementation-of-an-in-house-real-time-reverse-transcription-pcr-assay-for-the-rapid-detection-of-the-sars-cov-2-marseille-4-variant\/","title":{"rendered":"Implementation of an In-House Real-Time Reverse Transcription-PCR Assay for the Rapid Detection of the SARS-CoV-2 Marseille-4 Variant"},"content":{"rendered":"<p><i>[Pre-print, not peer-reviewed]<\/i> A study evaluating an in-house, real-time RT-PCR (qPCR) assay to detect a SARS-CoV-2 variant with an S477N substitution in the receptor binding domain associated with increased binding affinity to ACE2 found that the assay reliably detected this variant. All 6 cDNA samples from the variant strains tested positive using the assay, whereas all 32 cDNA samples from other variants tested negative. In addition, 93% of respiratory samples identified by next-generation sequencing as containing the variant strain were positive and 0\/26 samples containing non-variant strains were positive.<span class=\"Apple-converted-space\">\u00a0<\/span><\/p>\n<p><i>Bedotto et al. (Feb 8, 2021). Implementation of an In-House Real-Time Reverse Transcription-PCR Assay for the Rapid Detection of the SARS-CoV-2 Marseille-4 Variant. Pre-print downloaded Feb 8 from <\/i><a href=\"https:\/\/doi.org\/10.1101\/2021.02.03.21250823\">https:\/\/doi.org\/10.1101\/2021.02.03.21250823<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>[Pre-print, not peer-reviewed] A study evaluating an in-house, real-time RT-PCR (qPCR) assay to detect a SARS-CoV-2 variant with an S477N substitution in the receptor binding domain associated with increased binding affinity to ACE2 found that the assay reliably detected this variant. All 6 cDNA samples from the variant strains tested positive using the assay, whereas&#8230;<\/p>\n<div><a class=\"more\" href=\"https:\/\/depts.washington.edu\/pandemicalliance\/2021\/02\/08\/implementation-of-an-in-house-real-time-reverse-transcription-pcr-assay-for-the-rapid-detection-of-the-sars-cov-2-marseille-4-variant\/\">Read more<\/a><\/div>\n","protected":false},"author":7,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":"","_links_to":"","_links_to_target":""},"categories":[6],"tags":[27,159],"topic":[19],"class_list":["post-3766","post","type-post","status-publish","format-standard","hentry","category-article-summary","tag-testing","tag-variants","topic-testing-and-treatment"],"_links":{"self":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/3766","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/users\/7"}],"replies":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/comments?post=3766"}],"version-history":[{"count":1,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/3766\/revisions"}],"predecessor-version":[{"id":3767,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/3766\/revisions\/3767"}],"wp:attachment":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/media?parent=3766"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/categories?post=3766"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/tags?post=3766"},{"taxonomy":"topic","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/topic?post=3766"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}