{"id":5851,"date":"2020-04-10T14:16:43","date_gmt":"2020-04-10T21:16:43","guid":{"rendered":"https:\/\/depts.washington.edu\/pandemicalliance\/?p=5851"},"modified":"2021-03-05T14:17:31","modified_gmt":"2021-03-05T22:17:31","slug":"optimization-of-sars-cov-2-detection-by-rt-qpcr-without-rna-extraction","status":"publish","type":"post","link":"https:\/\/depts.washington.edu\/pandemicalliance\/2020\/04\/10\/optimization-of-sars-cov-2-detection-by-rt-qpcr-without-rna-extraction\/","title":{"rendered":"Optimization of SARS-CoV-2 detection by RT-QPCR without RNA extraction"},"content":{"rendered":"<ul>\n<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">The authors evaluated a direct RT-QPCR method without RNA extraction and the influence of swab storage media on detection.\u00a0 Their SeeGene\u2019s assay provided similar efficiency to RealStar\u00ae.<\/span><\/li>\n<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">The authors conclude that RNA extraction may not be necessary if samples are stored in UTM or molecular water; and also recommend that samples be stored in saline solution or Hanks medium.<\/span><\/li>\n<\/ul>\n<p><i><span style=\"font-weight: 400\">Merindol et al. (Apr 10, 2020). Optimization of SARS-CoV-2 detection by RT-QPCR without RNA extraction. Pre-print downloaded Apr 10 from<\/span><\/i> <a href=\"https:\/\/doi.org\/10.1101\/2020.04.06.028902\"><span style=\"font-weight: 400\">https:\/\/doi.org\/10.1101\/2020.04.06.028902<\/span><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>The authors evaluated a direct RT-QPCR method without RNA extraction and the influence of swab storage media on detection.\u00a0 Their SeeGene\u2019s assay provided similar efficiency to RealStar\u00ae. The authors conclude that RNA extraction may not be necessary if samples are stored in UTM or molecular water; and also recommend that samples be stored in saline&#8230;<\/p>\n<div><a class=\"more\" href=\"https:\/\/depts.washington.edu\/pandemicalliance\/2020\/04\/10\/optimization-of-sars-cov-2-detection-by-rt-qpcr-without-rna-extraction\/\">Read more<\/a><\/div>\n","protected":false},"author":8,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":"","_links_to":"","_links_to_target":""},"categories":[6],"tags":[],"topic":[19],"class_list":["post-5851","post","type-post","status-publish","format-standard","hentry","category-article-summary","topic-testing-and-treatment"],"_links":{"self":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/5851","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/comments?post=5851"}],"version-history":[{"count":1,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/5851\/revisions"}],"predecessor-version":[{"id":5852,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/5851\/revisions\/5852"}],"wp:attachment":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/media?parent=5851"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/categories?post=5851"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/tags?post=5851"},{"taxonomy":"topic","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/topic?post=5851"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}