{"id":6146,"date":"2020-04-20T14:37:31","date_gmt":"2020-04-20T21:37:31","guid":{"rendered":"https:\/\/depts.washington.edu\/pandemicalliance\/?p=6146"},"modified":"2021-03-09T14:38:32","modified_gmt":"2021-03-09T22:38:32","slug":"evaluation-of-antibody-testing-for-sars-cov-2-using-elisa-and-lateral-flow-immunoassays","status":"publish","type":"post","link":"https:\/\/depts.washington.edu\/pandemicalliance\/2020\/04\/20\/evaluation-of-antibody-testing-for-sars-cov-2-using-elisa-and-lateral-flow-immunoassays\/","title":{"rendered":"Evaluation of antibody testing for SARS-CoV-2 using ELISA and lateral flow immunoassays"},"content":{"rendered":"<ul>\n<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Recognizing the need for robust antibody detection testing, Crook tested plasma for SARS-CoV-2 IgM and IgG antibodies by ELISA and 9 different commercially available lateral flow immunoassay (LFIA) devices. ELISA has a sensitivity of 85% (34\/40) \u226510 after symptom onset and a specificity of 100% (142\/142), whereas the performance of LFIA was much lower with point estimates for sensitivity ranging from 55-70% and specificity from 95-100%.\u00a0<\/span><\/li>\n<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">If antibody tests are deployed as an individual-level approach to inform release from quarantine, then high-specificity is essential, as false-positive results return non-immune individuals to risk of exposure. The author concludes that LFIA devices may be useful for population-level surveys, but are currently inadequate for individual patient applications.\u00a0\u00a0<\/span><\/li>\n<\/ul>\n<p><i><span style=\"font-weight: 400\">Crook (Apr 20, 2020). Evaluation of antibody testing for SARS-CoV-2 using ELISA and lateral flow immunoassays. Pre-print downloaded Apr 20 from <\/span><\/i><a href=\"https:\/\/www.medrxiv.org\/content\/10.1101\/2020.04.15.20066407v1\"><span style=\"font-weight: 400\">https:\/\/doi.org\/10.1101\/2020.04.15.20066407<\/span><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Recognizing the need for robust antibody detection testing, Crook tested plasma for SARS-CoV-2 IgM and IgG antibodies by ELISA and 9 different commercially available lateral flow immunoassay (LFIA) devices. ELISA has a sensitivity of 85% (34\/40) \u226510 after symptom onset and a specificity of 100% (142\/142), whereas the performance of LFIA was much lower with&#8230;<\/p>\n<div><a class=\"more\" href=\"https:\/\/depts.washington.edu\/pandemicalliance\/2020\/04\/20\/evaluation-of-antibody-testing-for-sars-cov-2-using-elisa-and-lateral-flow-immunoassays\/\">Read more<\/a><\/div>\n","protected":false},"author":8,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":"","_links_to":"","_links_to_target":""},"categories":[6],"tags":[],"topic":[19],"class_list":["post-6146","post","type-post","status-publish","format-standard","hentry","category-article-summary","topic-testing-and-treatment"],"_links":{"self":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/6146","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/comments?post=6146"}],"version-history":[{"count":1,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/6146\/revisions"}],"predecessor-version":[{"id":6147,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/6146\/revisions\/6147"}],"wp:attachment":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/media?parent=6146"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/categories?post=6146"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/tags?post=6146"},{"taxonomy":"topic","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/topic?post=6146"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}