{"id":6226,"date":"2020-04-23T16:05:40","date_gmt":"2020-04-23T23:05:40","guid":{"rendered":"https:\/\/depts.washington.edu\/pandemicalliance\/?p=6226"},"modified":"2021-03-09T16:06:49","modified_gmt":"2021-03-10T00:06:49","slug":"detection-of-sars-cov-2-rna-by-direct-rt-qpcr-on-nasopharyngeal-specimens-without-extraction-of-viral-rna","status":"publish","type":"post","link":"https:\/\/depts.washington.edu\/pandemicalliance\/2020\/04\/23\/detection-of-sars-cov-2-rna-by-direct-rt-qpcr-on-nasopharyngeal-specimens-without-extraction-of-viral-rna\/","title":{"rendered":"Detection of SARS-CoV-2 RNA by direct RT-qPCR on nasopharyngeal specimens without extraction of viral RNA."},"content":{"rendered":"<ul>\n<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">In order to circumvent the limited availability of RNA extraction reagents and to reduce the cost and turn-around time of the assay, the authors describe a new protocol for direct RT-qPCR to detect SARS-CoV-2 in nasopharyngeal swabs. The new approach provides higher analytic sensitivity for detection than many other commercial and laboratory-developed methods, and the rate of RT-qPCR inhibition was similar to the standard approach.\u00a0<\/span><\/li>\n<\/ul>\n<p><i><span style=\"font-weight: 400\">Hasan et al. (Apr 18, 2020). Detection of SARS-CoV-2 RNA by direct RT-qPCR on nasopharyngeal specimens without extraction of viral RNA. Pre-print downloaded Apr 23 from <\/span><\/i><a href=\"https:\/\/doi.org\/10.1101\/2020.04.18.20070755\"><span style=\"font-weight: 400\">https:\/\/doi.org\/10.1101\/2020.04.18.20070755<\/span><\/a><i><span style=\"font-weight: 400\">\u00a0<\/span><\/i><\/p>\n","protected":false},"excerpt":{"rendered":"<p>In order to circumvent the limited availability of RNA extraction reagents and to reduce the cost and turn-around time of the assay, the authors describe a new protocol for direct RT-qPCR to detect SARS-CoV-2 in nasopharyngeal swabs. The new approach provides higher analytic sensitivity for detection than many other commercial and laboratory-developed methods, and the&#8230;<\/p>\n<div><a class=\"more\" href=\"https:\/\/depts.washington.edu\/pandemicalliance\/2020\/04\/23\/detection-of-sars-cov-2-rna-by-direct-rt-qpcr-on-nasopharyngeal-specimens-without-extraction-of-viral-rna\/\">Read more<\/a><\/div>\n","protected":false},"author":8,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":"","_links_to":"","_links_to_target":""},"categories":[6],"tags":[],"topic":[19],"class_list":["post-6226","post","type-post","status-publish","format-standard","hentry","category-article-summary","topic-testing-and-treatment"],"_links":{"self":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/6226","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/comments?post=6226"}],"version-history":[{"count":1,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/6226\/revisions"}],"predecessor-version":[{"id":6227,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/6226\/revisions\/6227"}],"wp:attachment":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/media?parent=6226"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/categories?post=6226"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/tags?post=6226"},{"taxonomy":"topic","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/topic?post=6226"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}