{"id":6574,"date":"2021-03-15T13:46:56","date_gmt":"2021-03-15T20:46:56","guid":{"rendered":"https:\/\/depts.washington.edu\/pandemicalliance\/?p=6574"},"modified":"2021-03-16T13:48:13","modified_gmt":"2021-03-16T20:48:13","slug":"specific-allelic-discrimination-of-n501y-and-other-sars-cov-2-mutations-by-ddpcr-detects-b-1-1-7-lineage-in-washington-state","status":"publish","type":"post","link":"https:\/\/depts.washington.edu\/pandemicalliance\/2021\/03\/15\/specific-allelic-discrimination-of-n501y-and-other-sars-cov-2-mutations-by-ddpcr-detects-b-1-1-7-lineage-in-washington-state\/","title":{"rendered":"Specific Allelic Discrimination of N501Y and Other SARS-CoV-2 Mutations by DdPCR Detects B.1.1.7 Lineage in Washington State"},"content":{"rendered":"<ul>\n<li style=\"font-weight: 400\"><i><span style=\"font-weight: 400\">[Pre-print, not peer-reviewed]<\/span><\/i> <span style=\"font-weight: 400\">A research team in Washington State described detection of the first cases of the B.1.1.7 SARS-CoV-2 variant in the state using a novel droplet reverse transcriptase digital-PCR (RT-ddPCR) assay that detects four mutations associated with the B.1.1.7 lineage. All four targets were detected in two specimens, and follow-up sequencing revealed 10 mutations in the S gene and phylogenetic clustering within the B.1.1.7 lineage.\u00a0The authors suggest that RT-ddPCR may support efficient surveillance of SARS-CoV-2 strains.\u00a0<\/span><\/li>\n<\/ul>\n<p><i><span style=\"font-weight: 400\">Perchetti et al.\u00a0(Mar 12, 2021). Specific Allelic Discrimination of N501Y and Other SARS-CoV-2 Mutations by DdPCR Detects B.1.1.7 Lineage in Washington State. Pre-print downloaded Mar 15 from <\/span><\/i><a href=\"https:\/\/doi.org\/10.1101\/2021.03.10.21253321\"><span style=\"font-weight: 400\">https:\/\/doi.org\/10.1101\/2021.03.10.21253321<\/span><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>[Pre-print, not peer-reviewed] A research team in Washington State described detection of the first cases of the B.1.1.7 SARS-CoV-2 variant in the state using a novel droplet reverse transcriptase digital-PCR (RT-ddPCR) assay that detects four mutations associated with the B.1.1.7 lineage. All four targets were detected in two specimens, and follow-up sequencing revealed 10 mutations&#8230;<\/p>\n<div><a class=\"more\" href=\"https:\/\/depts.washington.edu\/pandemicalliance\/2021\/03\/15\/specific-allelic-discrimination-of-n501y-and-other-sars-cov-2-mutations-by-ddpcr-detects-b-1-1-7-lineage-in-washington-state\/\">Read more<\/a><\/div>\n","protected":false},"author":8,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":"","_links_to":"","_links_to_target":""},"categories":[6],"tags":[27,59],"topic":[19],"class_list":["post-6574","post","type-post","status-publish","format-standard","hentry","category-article-summary","tag-testing","tag-wa","topic-testing-and-treatment"],"_links":{"self":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/6574","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/comments?post=6574"}],"version-history":[{"count":1,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/6574\/revisions"}],"predecessor-version":[{"id":6575,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/6574\/revisions\/6575"}],"wp:attachment":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/media?parent=6574"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/categories?post=6574"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/tags?post=6574"},{"taxonomy":"topic","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/topic?post=6574"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}