{"id":6611,"date":"2020-05-04T14:21:06","date_gmt":"2020-05-04T21:21:06","guid":{"rendered":"https:\/\/depts.washington.edu\/pandemicalliance\/?p=6611"},"modified":"2021-03-16T14:22:12","modified_gmt":"2021-03-16T21:22:12","slug":"evaluation-of-covid-19-rt-qpcr-test-in-multi-sample-pools-2","status":"publish","type":"post","link":"https:\/\/depts.washington.edu\/pandemicalliance\/2020\/05\/04\/evaluation-of-covid-19-rt-qpcr-test-in-multi-sample-pools-2\/","title":{"rendered":"Evaluation of\u00a0COVID-19 RT-QPCR Test in Multi-Sample Pools"},"content":{"rendered":"<ul>\n<li data-leveltext=\"\uf0b7\" data-font=\"Symbol\" data-listid=\"38\" data-aria-posinset=\"2\" data-aria-level=\"1\"><span data-contrast=\"auto\">Many healthcare systems are experiencing shortages of reagents for SARS-CoV-2 diagnostic testing. These authors were able to detect a single positive sample in pools of up to 32 negative samples<\/span><span data-contrast=\"auto\">, either before\u00a0<\/span><span data-contrast=\"auto\">or after RNA extraction,\u00a0<\/span><span data-contrast=\"auto\">with an estimated false negative rate of 10%<\/span><span data-contrast=\"auto\">. Single positive samples could also be recovered from pools of 64 (the highest pool size tested) with additional amplification cycles.\u00a0<\/span><span data-contrast=\"auto\">Pooled testing could be applied immediately to expand screening capacities.\u00a0<\/span><span data-ccp-props=\"{&quot;134233279&quot;:true,&quot;201341983&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/li>\n<\/ul>\n<p><i><span data-contrast=\"none\">Yelin et al. (May<\/span><\/i><i><span data-contrast=\"none\">\u00a0<\/span><\/i><i><span data-contrast=\"none\">2<\/span><\/i><i><span data-contrast=\"none\">, 2020). Evaluation of\u00a0COVID-19 RT-QPCR Test in Multi-Sample Pools. Clinical Infectious Diseases<\/span><\/i><i><span data-contrast=\"auto\">.\u00a0<\/span><\/i><a href=\"https:\/\/doi.org\/10.1093\/cid\/ciaa531\"><i><span data-contrast=\"none\">https:\/\/doi.org\/10.1093\/cid\/ciaa531<\/span><\/i><\/a><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559685&quot;:720,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Many healthcare systems are experiencing shortages of reagents for SARS-CoV-2 diagnostic testing. These authors were able to detect a single positive sample in pools of up to 32 negative samples, either before\u00a0or after RNA extraction,\u00a0with an estimated false negative rate of 10%. Single positive samples could also be recovered from pools of 64 (the highest&#8230;<\/p>\n<div><a class=\"more\" href=\"https:\/\/depts.washington.edu\/pandemicalliance\/2020\/05\/04\/evaluation-of-covid-19-rt-qpcr-test-in-multi-sample-pools-2\/\">Read more<\/a><\/div>\n","protected":false},"author":8,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":"","_links_to":"","_links_to_target":""},"categories":[6],"tags":[],"topic":[19],"class_list":["post-6611","post","type-post","status-publish","format-standard","hentry","category-article-summary","topic-testing-and-treatment"],"_links":{"self":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/6611","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/comments?post=6611"}],"version-history":[{"count":1,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/6611\/revisions"}],"predecessor-version":[{"id":6612,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/6611\/revisions\/6612"}],"wp:attachment":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/media?parent=6611"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/categories?post=6611"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/tags?post=6611"},{"taxonomy":"topic","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/topic?post=6611"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}