{"id":7134,"date":"2020-05-27T17:22:28","date_gmt":"2020-05-28T00:22:28","guid":{"rendered":"https:\/\/depts.washington.edu\/pandemicalliance\/?p=7134"},"modified":"2021-03-19T17:23:10","modified_gmt":"2021-03-20T00:23:10","slug":"sample-pooling-as-a-strategy-of-sars-cov-2-nucleic-acid-screening-increases-the-false-negative-rate","status":"publish","type":"post","link":"https:\/\/depts.washington.edu\/pandemicalliance\/2020\/05\/27\/sample-pooling-as-a-strategy-of-sars-cov-2-nucleic-acid-screening-increases-the-false-negative-rate\/","title":{"rendered":"Sample Pooling as a Strategy of SARS-COV-2 Nucleic Acid Screening Increases the False-Negative Rate"},"content":{"rendered":"<ul>\n<li data-leveltext=\"\uf0b7\" data-font=\"Symbol\" data-listid=\"34\" data-aria-posinset=\"2019\" data-aria-level=\"1\"><i><span data-contrast=\"none\">[<\/span><\/i><i><span data-contrast=\"none\">pre-print, not peer reviewed<\/span><\/i><i><span data-contrast=\"none\">]<\/span><\/i><span data-contrast=\"auto\">\u00a0<\/span><span data-contrast=\"auto\">Gan et al. diluted three SARS-CoV-2 positive samples (2 with high viral load<\/span><span data-contrast=\"auto\">\u00a0and<\/span><span data-contrast=\"auto\">\u00a01 with low viral load) with 8<\/span><span data-contrast=\"auto\">,<\/span><span data-contrast=\"auto\">094<\/span><span data-contrast=\"auto\">\u00a0negative samples or\u00a0<\/span><span data-contrast=\"auto\">double distilled water\u00a0<\/span><span data-contrast=\"auto\">to form RNA pools.\u00a0<\/span><span data-contrast=\"auto\">SARS-CoV-2 was detected in pools from\u00a0<\/span><span data-contrast=\"auto\">high viral load samples up to 1\/1000 dilution, while\u00a0<\/span><span data-contrast=\"auto\">the low viral load sample<\/span><span data-contrast=\"auto\">\u00a0<\/span><span data-contrast=\"auto\">could only be observed at a low dilution ratio<\/span><span data-contrast=\"auto\">.<\/span><span data-contrast=\"auto\">\u00a0<\/span><span data-contrast=\"auto\">Based on these findings,\u00a0<\/span><span data-contrast=\"auto\">pooling\u00a0<\/span><span data-contrast=\"auto\">samples for\u00a0<\/span><span data-contrast=\"auto\">surveillance may lead to false negatives<\/span><span data-contrast=\"auto\">\u00a0for samples with low viral load<\/span><span data-contrast=\"auto\">.<\/span><span data-ccp-props=\"{&quot;134233279&quot;:true,&quot;201341983&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/li>\n<\/ul>\n<p><i><span data-contrast=\"none\">Gan et al. (May 26, 2020). Sample Pooling as a Strategy of SARS-COV-2 Nucleic Acid Screening Increases the False-Negative Rate. Pre-print\u00a0downloaded May 27 from\u00a0<\/span><\/i><a href=\"https:\/\/doi.org\/10.1101\/2020.05.18.20106138\"><span data-contrast=\"none\">https:\/\/doi.org\/10.1101\/2020.05.18.20106138<\/span><\/a><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559685&quot;:720,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p>[pre-print, not peer reviewed]\u00a0Gan et al. diluted three SARS-CoV-2 positive samples (2 with high viral load\u00a0and\u00a01 with low viral load) with 8,094\u00a0negative samples or\u00a0double distilled water\u00a0to form RNA pools.\u00a0SARS-CoV-2 was detected in pools from\u00a0high viral load samples up to 1\/1000 dilution, while\u00a0the low viral load sample\u00a0could only be observed at a low dilution ratio.\u00a0Based on&#8230;<\/p>\n<div><a class=\"more\" href=\"https:\/\/depts.washington.edu\/pandemicalliance\/2020\/05\/27\/sample-pooling-as-a-strategy-of-sars-cov-2-nucleic-acid-screening-increases-the-false-negative-rate\/\">Read more<\/a><\/div>\n","protected":false},"author":8,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":"","_links_to":"","_links_to_target":""},"categories":[6],"tags":[],"topic":[19],"class_list":["post-7134","post","type-post","status-publish","format-standard","hentry","category-article-summary","topic-testing-and-treatment"],"_links":{"self":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/7134","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/comments?post=7134"}],"version-history":[{"count":1,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/7134\/revisions"}],"predecessor-version":[{"id":7135,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/7134\/revisions\/7135"}],"wp:attachment":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/media?parent=7134"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/categories?post=7134"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/tags?post=7134"},{"taxonomy":"topic","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/topic?post=7134"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}