{"id":7309,"date":"2020-05-03T13:09:57","date_gmt":"2020-05-03T20:09:57","guid":{"rendered":"https:\/\/depts.washington.edu\/pandemicalliance\/?p=7309"},"modified":"2021-03-24T13:10:46","modified_gmt":"2021-03-24T20:10:46","slug":"direct-diagnostic-testing-of-sars-cov-2-without-the-need-for-prior-rna-extraction","status":"publish","type":"post","link":"https:\/\/depts.washington.edu\/pandemicalliance\/2020\/05\/03\/direct-diagnostic-testing-of-sars-cov-2-without-the-need-for-prior-rna-extraction\/","title":{"rendered":"Direct Diagnostic Testing of SARS-CoV-2 without the Need for Prior RNA Extraction"},"content":{"rendered":"<ul>\n<li data-leveltext=\"\uf0b7\" data-font=\"Symbol\" data-listid=\"34\" data-aria-posinset=\"2\" data-aria-level=\"1\"><i><span data-contrast=\"none\">[pre-print, not peer reviewed]<\/span><\/i><span data-contrast=\"auto\">\u00a0Wei et al. report on a novel method for nucleic acid amplification testing (NAAT) for point-of-care SARS-CoV-2 diagnostic testing<\/span><span data-contrast=\"auto\">\u00a0[Loop-mediated isothermal amplification (LAMP)]<\/span><span data-contrast=\"auto\">. This assay can be run directly on transport media following a nasopharyngeal swab without requiring an RNA extraction step prior to amplification of the viral\u00a0RNA, and\u00a0provides\u00a0<\/span><span data-contrast=\"auto\">results in 30 minutes. The authors reported a sensitivity of 85% and specificity of 100% based on 10 positive and 10 negative samples.<\/span><span data-ccp-props=\"{&quot;134233279&quot;:true,&quot;201341983&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/li>\n<\/ul>\n<p><i><span data-contrast=\"none\">Wei et al. (June 2, 2020). Direct Diagnostic Testing of SARS-CoV-2 without the Need for Prior RNA Extraction.\u00a0Preprint\u00a0downloaded\u00a0June\u00a03 from\u00a0<\/span><\/i><a href=\"https:\/\/doi.org\/10.1101\/2020.05.28.20115220\"><span data-contrast=\"none\">https:\/\/doi.org\/10.1101\/2020.05.28.20115220<\/span><\/a><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559685&quot;:720,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p>[pre-print, not peer reviewed]\u00a0Wei et al. report on a novel method for nucleic acid amplification testing (NAAT) for point-of-care SARS-CoV-2 diagnostic testing\u00a0[Loop-mediated isothermal amplification (LAMP)]. This assay can be run directly on transport media following a nasopharyngeal swab without requiring an RNA extraction step prior to amplification of the viral\u00a0RNA, and\u00a0provides\u00a0results in 30 minutes. The&#8230;<\/p>\n<div><a class=\"more\" href=\"https:\/\/depts.washington.edu\/pandemicalliance\/2020\/05\/03\/direct-diagnostic-testing-of-sars-cov-2-without-the-need-for-prior-rna-extraction\/\">Read more<\/a><\/div>\n","protected":false},"author":8,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":"","_links_to":"","_links_to_target":""},"categories":[6],"tags":[],"topic":[19],"class_list":["post-7309","post","type-post","status-publish","format-standard","hentry","category-article-summary","topic-testing-and-treatment"],"_links":{"self":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/7309","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/comments?post=7309"}],"version-history":[{"count":1,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/7309\/revisions"}],"predecessor-version":[{"id":7310,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/7309\/revisions\/7310"}],"wp:attachment":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/media?parent=7309"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/categories?post=7309"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/tags?post=7309"},{"taxonomy":"topic","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/topic?post=7309"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}