{"id":7400,"date":"2020-06-08T15:41:48","date_gmt":"2020-06-08T22:41:48","guid":{"rendered":"https:\/\/depts.washington.edu\/pandemicalliance\/?p=7400"},"modified":"2021-03-24T15:42:38","modified_gmt":"2021-03-24T22:42:38","slug":"identification-of-nsp1-gene-as-the-target-of-sars-cov-2-real-time-rt-pcr-using-nanopore-whole-genome-sequencing","status":"publish","type":"post","link":"https:\/\/depts.washington.edu\/pandemicalliance\/2020\/06\/08\/identification-of-nsp1-gene-as-the-target-of-sars-cov-2-real-time-rt-pcr-using-nanopore-whole-genome-sequencing\/","title":{"rendered":"Identification of Nsp1 Gene as the Target of SARS-CoV-2 Real-Time RT-PCR Using Nanopore Whole Genome Sequencing"},"content":{"rendered":"<ul>\n<li data-leveltext=\"\uf0b7\" data-font=\"Symbol\" data-listid=\"36\" data-aria-posinset=\"1\" data-aria-level=\"1\"><span data-contrast=\"auto\">Chan et al. found that\u00a0<\/span><span data-contrast=\"auto\">SARS-CoV-2 mutations in current binding sites for PCR tests may compromise their sensitivity\u00a0<\/span><span data-contrast=\"auto\">and<\/span><span data-contrast=\"auto\">\u00a0identified the nsp1 gene as a novel target. An RT-PCR assay developed using this\u00a0<\/span><span data-contrast=\"auto\">target<\/span><span data-contrast=\"auto\">\u00a0had a sensitivity of 93.1% and a specificity of 100%.\u00a0\u00a0<\/span><span data-ccp-props=\"{&quot;134233279&quot;:true,&quot;201341983&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/li>\n<\/ul>\n<p><i><span data-contrast=\"none\">Chan et al. (June 202<\/span><\/i><i><span data-contrast=\"none\">0). Identification of Nsp1 Gene as the Target of SARS-CoV-2 Real-Time RT-PCR Using Nanopore Whole Genome Sequencing. Journal of Medical Virology.\u00a0<\/span><\/i><a href=\"https:\/\/doi.org\/10.1002\/jmv.26140\"><i><span data-contrast=\"none\">https:\/\/doi.org\/10.1002\/jmv.26140<\/span><\/i><\/a><span data-ccp-props=\"{&quot;134233279&quot;:true,&quot;201341983&quot;:0,&quot;335559685&quot;:720,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Chan et al. found that\u00a0SARS-CoV-2 mutations in current binding sites for PCR tests may compromise their sensitivity\u00a0and\u00a0identified the nsp1 gene as a novel target. An RT-PCR assay developed using this\u00a0target\u00a0had a sensitivity of 93.1% and a specificity of 100%.\u00a0\u00a0\u00a0 Chan et al. (June 2020). Identification of Nsp1 Gene as the Target of SARS-CoV-2 Real-Time RT-PCR&#8230;<\/p>\n<div><a class=\"more\" href=\"https:\/\/depts.washington.edu\/pandemicalliance\/2020\/06\/08\/identification-of-nsp1-gene-as-the-target-of-sars-cov-2-real-time-rt-pcr-using-nanopore-whole-genome-sequencing\/\">Read more<\/a><\/div>\n","protected":false},"author":8,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":"","_links_to":"","_links_to_target":""},"categories":[6],"tags":[],"topic":[19],"class_list":["post-7400","post","type-post","status-publish","format-standard","hentry","category-article-summary","topic-testing-and-treatment"],"_links":{"self":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/7400","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/comments?post=7400"}],"version-history":[{"count":1,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/7400\/revisions"}],"predecessor-version":[{"id":7401,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/7400\/revisions\/7401"}],"wp:attachment":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/media?parent=7400"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/categories?post=7400"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/tags?post=7400"},{"taxonomy":"topic","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/topic?post=7400"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}