{"id":7542,"date":"2020-06-15T14:57:40","date_gmt":"2020-06-15T21:57:40","guid":{"rendered":"https:\/\/depts.washington.edu\/pandemicalliance\/?p=7542"},"modified":"2021-03-25T14:58:21","modified_gmt":"2021-03-25T21:58:21","slug":"multiple-assays-in-a-real-time-rt-pcr-sars-cov-2-panel-can-mitigate-the-risk-of-loss-of-sensitivity-by-new-genomic-variants-during-the-covid-19-outbreak","status":"publish","type":"post","link":"https:\/\/depts.washington.edu\/pandemicalliance\/2020\/06\/15\/multiple-assays-in-a-real-time-rt-pcr-sars-cov-2-panel-can-mitigate-the-risk-of-loss-of-sensitivity-by-new-genomic-variants-during-the-covid-19-outbreak\/","title":{"rendered":"Multiple Assays in a Real-Time RT-PCR SARS-CoV-2 Panel Can Mitigate the Risk of Loss of Sensitivity by New Genomic Variants during the COVID-19 Outbreak"},"content":{"rendered":"<ul>\n<li data-leveltext=\"\uf0b7\" data-font=\"Symbol\" data-listid=\"34\" data-aria-posinset=\"2019\" data-aria-level=\"1\"><span data-contrast=\"auto\">Penarrubia\u00a0et al.\u00a0<\/span><span data-contrast=\"auto\">assessed the effect of accumulated genetic variability of SARS-CoV-2 on sensitivity of five SARS-CoV-2 PCR assay<\/span><span data-contrast=\"auto\">s<\/span><span data-contrast=\"auto\">. A total of 11,627 (34%) genomes includ<\/span><span data-contrast=\"auto\">ed<\/span><span data-contrast=\"auto\">\u00a0single mutations\u00a0<\/span><span data-contrast=\"auto\">that\u00a0<\/span><span data-contrast=\"auto\">affected annealing of a PCR assay. Variations in 8,773 (2<\/span><span data-contrast=\"auto\">6<\/span><span data-contrast=\"auto\">%) genomes were considered high risk, whereas\u00a0<\/span><span data-contrast=\"auto\">an\u00a0<\/span><span data-contrast=\"auto\">additional 2,854 (8.43%) genomes\u00a0<\/span><span data-contrast=\"auto\">had\u00a0<\/span><span data-contrast=\"auto\">single mutations\u00a0<\/span><span data-contrast=\"auto\">that were not\u00a0<\/span><span data-contrast=\"auto\">predicted\u00a0<\/span><span data-contrast=\"auto\">affect<\/span><span data-contrast=\"auto\">\u00a0sensitivity.\u00a0<\/span><span data-ccp-props=\"{&quot;134233279&quot;:true,&quot;201341983&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/li>\n<li data-leveltext=\"\uf0b7\" data-font=\"Symbol\" data-listid=\"34\" data-aria-posinset=\"2019\" data-aria-level=\"1\"><span data-contrast=\"auto\">T<\/span><span data-contrast=\"auto\">argeting more than one region in the viral genome for SARS-CoV-2 detection\u00a0<\/span><span data-contrast=\"auto\">may\u00a0<\/span><span data-contrast=\"auto\">mitigate the risk of loss of sensitivity due to the unknown mutation rate during this SARS-CoV-2 outbreak.<\/span><span data-ccp-props=\"{&quot;134233279&quot;:true,&quot;201341983&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/li>\n<\/ul>\n<p><i><span data-contrast=\"none\">Penarrubia\u00a0et al. (June 8, 2020). Multiple Assays in a Real-Time RT-PCR SARS-CoV-2 Panel Can Mitigate the Risk of Loss of Sensitivity by New Genomic Variants during the COVID-19 Outbreak. International Journal of Infectious Diseases.<\/span><\/i><span data-contrast=\"auto\">\u00a0<\/span><a href=\"https:\/\/doi.org\/10.1016\/j.ijid.2020.06.027\"><span data-contrast=\"none\">https:\/\/doi.org\/10.1016\/j.ijid.2020.06.027<\/span><\/a><span data-ccp-props=\"{&quot;134233279&quot;:true,&quot;201341983&quot;:0,&quot;335559685&quot;:720,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Penarrubia\u00a0et al.\u00a0assessed the effect of accumulated genetic variability of SARS-CoV-2 on sensitivity of five SARS-CoV-2 PCR assays. A total of 11,627 (34%) genomes included\u00a0single mutations\u00a0that\u00a0affected annealing of a PCR assay. Variations in 8,773 (26%) genomes were considered high risk, whereas\u00a0an\u00a0additional 2,854 (8.43%) genomes\u00a0had\u00a0single mutations\u00a0that were not\u00a0predicted\u00a0affect\u00a0sensitivity.\u00a0\u00a0 Targeting more than one region in the viral genome&#8230;<\/p>\n<div><a class=\"more\" href=\"https:\/\/depts.washington.edu\/pandemicalliance\/2020\/06\/15\/multiple-assays-in-a-real-time-rt-pcr-sars-cov-2-panel-can-mitigate-the-risk-of-loss-of-sensitivity-by-new-genomic-variants-during-the-covid-19-outbreak\/\">Read more<\/a><\/div>\n","protected":false},"author":8,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":"","_links_to":"","_links_to_target":""},"categories":[6],"tags":[],"topic":[19],"class_list":["post-7542","post","type-post","status-publish","format-standard","hentry","category-article-summary","topic-testing-and-treatment"],"_links":{"self":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/7542","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/comments?post=7542"}],"version-history":[{"count":1,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/7542\/revisions"}],"predecessor-version":[{"id":7543,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/7542\/revisions\/7543"}],"wp:attachment":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/media?parent=7542"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/categories?post=7542"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/tags?post=7542"},{"taxonomy":"topic","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/topic?post=7542"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}