{"id":7627,"date":"2020-06-18T15:45:26","date_gmt":"2020-06-18T22:45:26","guid":{"rendered":"https:\/\/depts.washington.edu\/pandemicalliance\/?p=7627"},"modified":"2021-03-25T15:46:07","modified_gmt":"2021-03-25T22:46:07","slug":"real-time-reverse-transcription-loop-mediated-isothermal-amplification-for-rapid-detection-of-sars-cov-2","status":"publish","type":"post","link":"https:\/\/depts.washington.edu\/pandemicalliance\/2020\/06\/18\/real-time-reverse-transcription-loop-mediated-isothermal-amplification-for-rapid-detection-of-sars-cov-2\/","title":{"rendered":"Real-Time Reverse Transcription Loop-Mediated Isothermal Amplification for Rapid Detection of SARS-CoV-2"},"content":{"rendered":"<ul>\n<li data-leveltext=\"\uf0b7\" data-font=\"Symbol\" data-listid=\"34\" data-aria-posinset=\"2019\" data-aria-level=\"1\"><span data-contrast=\"auto\">Lau et al. developed a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for rapid detection of SARS-CoV-2.\u00a0<\/span><span data-contrast=\"auto\">In comparison to<\/span><span data-contrast=\"auto\">\u00a0the RT-qPCR assay, the investigators reported\u00a0<\/span><span data-contrast=\"auto\">that the RT-LAMP assay was 2.5 times faster<\/span><span data-contrast=\"auto\">\u00a0and\u00a0<\/span><span data-contrast=\"auto\">had\u00a0<\/span><span data-contrast=\"auto\">a clinical sensitivity and specificity of 100%.<\/span><span data-contrast=\"auto\">\u00a0However, the RT-LAMP assay had a\u00a0<\/span><span data-contrast=\"auto\">5-fold higher limit of detection,\u00a0<\/span><span data-ccp-props=\"{&quot;134233279&quot;:true,&quot;201341983&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/li>\n<\/ul>\n<p><i><span data-contrast=\"none\">Lau et al. (June 3, 2020). Real-Time Reverse Transcription Loop-Mediated Isothermal Amplification for Rapid Detection of SARS-CoV-2.\u00a0<\/span><\/i><i><span data-contrast=\"none\">PeerJ<\/span><\/i><i><span data-contrast=\"none\">.\u00a0<\/span><\/i><a href=\"https:\/\/doi.org\/10.7717\/peerj.9278\"><span data-contrast=\"none\">https:\/\/doi.org\/10.7717\/peerj.9278<\/span><\/a><span data-ccp-props=\"{&quot;134233279&quot;:true,&quot;201341983&quot;:0,&quot;335559685&quot;:720,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Lau et al. developed a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for rapid detection of SARS-CoV-2.\u00a0In comparison to\u00a0the RT-qPCR assay, the investigators reported\u00a0that the RT-LAMP assay was 2.5 times faster\u00a0and\u00a0had\u00a0a clinical sensitivity and specificity of 100%.\u00a0However, the RT-LAMP assay had a\u00a05-fold higher limit of detection,\u00a0\u00a0 Lau et al. (June 3, 2020). Real-Time Reverse Transcription&#8230;<\/p>\n<div><a class=\"more\" href=\"https:\/\/depts.washington.edu\/pandemicalliance\/2020\/06\/18\/real-time-reverse-transcription-loop-mediated-isothermal-amplification-for-rapid-detection-of-sars-cov-2\/\">Read more<\/a><\/div>\n","protected":false},"author":8,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":"","_links_to":"","_links_to_target":""},"categories":[6],"tags":[],"topic":[19],"class_list":["post-7627","post","type-post","status-publish","format-standard","hentry","category-article-summary","topic-testing-and-treatment"],"_links":{"self":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/7627","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/comments?post=7627"}],"version-history":[{"count":1,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/7627\/revisions"}],"predecessor-version":[{"id":7628,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/7627\/revisions\/7628"}],"wp:attachment":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/media?parent=7627"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/categories?post=7627"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/tags?post=7627"},{"taxonomy":"topic","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/topic?post=7627"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}