{"id":7718,"date":"2020-06-22T12:04:00","date_gmt":"2020-06-22T19:04:00","guid":{"rendered":"https:\/\/depts.washington.edu\/pandemicalliance\/?p=7718"},"modified":"2021-03-26T12:04:40","modified_gmt":"2021-03-26T19:04:40","slug":"performance-assessment-of-sars-cov-2-pcr-assays-developed-by-who-referral-laboratories","status":"publish","type":"post","link":"https:\/\/depts.washington.edu\/pandemicalliance\/2020\/06\/22\/performance-assessment-of-sars-cov-2-pcr-assays-developed-by-who-referral-laboratories\/","title":{"rendered":"Performance Assessment of SARS-CoV-2 PCR Assays Developed by WHO Referral Laboratories"},"content":{"rendered":"<ul>\n<li data-leveltext=\"\uf0b7\" data-font=\"Symbol\" data-listid=\"34\" data-aria-posinset=\"2019\" data-aria-level=\"1\"><span data-contrast=\"auto\">Etievant<\/span><span data-contrast=\"auto\">\u00a0et al.\u00a0<\/span><span data-contrast=\"auto\">compared the performance of five RT-PCR-based methods developed by\u00a0<\/span><span data-contrast=\"auto\">World Health Organization\u00a0<\/span><span data-contrast=\"auto\">referral laboratories.\u00a0<\/span><span data-contrast=\"auto\">Three assays known as\u00a0<\/span><span data-contrast=\"auto\">N China CDC, N1 US CDC, and\u00a0<\/span><span data-contrast=\"auto\">RdRp<\/span><span data-contrast=\"auto\">\u00a0(IP2, IP4) were found to be the most sensitive assays on\u00a0<\/span><span data-contrast=\"auto\">based on\u00a0<\/span><span data-contrast=\"auto\">SARS-CoV-2 cell culture supernatants\u00a0<\/span><span data-contrast=\"auto\">and clinical respiratory samples. The E\u00a0<\/span><span data-contrast=\"auto\">Charite<\/span><span data-contrast=\"auto\">\u0301 and N2 US CDC assays had false-positive results from negative samples and negative controls.\u00a0<\/span><span data-ccp-props=\"{&quot;134233279&quot;:true,&quot;201341983&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/li>\n<\/ul>\n<p><i><span data-contrast=\"none\">Etievant<\/span><\/i><i><span data-contrast=\"none\">\u00a0et al. (June 16, 2020). Performance Assessment of SARS-CoV-2 PCR Assays Developed by WHO Referral Laboratories. Journal of Clinical Medicine.\u00a0<\/span><\/i><a href=\"https:\/\/doi.org\/10.3390\/jcm9061871\"><span data-contrast=\"none\">https:\/\/doi.org\/10.3390\/jcm9061871<\/span><\/a><i><span data-contrast=\"none\">\u00a0<\/span><\/i><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559685&quot;:720,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Etievant\u00a0et al.\u00a0compared the performance of five RT-PCR-based methods developed by\u00a0World Health Organization\u00a0referral laboratories.\u00a0Three assays known as\u00a0N China CDC, N1 US CDC, and\u00a0RdRp\u00a0(IP2, IP4) were found to be the most sensitive assays on\u00a0based on\u00a0SARS-CoV-2 cell culture supernatants\u00a0and clinical respiratory samples. The E\u00a0Charite\u0301 and N2 US CDC assays had false-positive results from negative samples and negative controls.\u00a0\u00a0&#8230;<\/p>\n<div><a class=\"more\" href=\"https:\/\/depts.washington.edu\/pandemicalliance\/2020\/06\/22\/performance-assessment-of-sars-cov-2-pcr-assays-developed-by-who-referral-laboratories\/\">Read more<\/a><\/div>\n","protected":false},"author":8,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":"","_links_to":"","_links_to_target":""},"categories":[6],"tags":[],"topic":[19],"class_list":["post-7718","post","type-post","status-publish","format-standard","hentry","category-article-summary","topic-testing-and-treatment"],"_links":{"self":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/7718","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/comments?post=7718"}],"version-history":[{"count":1,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/7718\/revisions"}],"predecessor-version":[{"id":7719,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/7718\/revisions\/7719"}],"wp:attachment":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/media?parent=7718"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/categories?post=7718"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/tags?post=7718"},{"taxonomy":"topic","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/topic?post=7718"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}