{"id":8037,"date":"2020-07-08T08:39:26","date_gmt":"2020-07-08T15:39:26","guid":{"rendered":"https:\/\/depts.washington.edu\/pandemicalliance\/?p=8037"},"modified":"2021-03-31T08:40:12","modified_gmt":"2021-03-31T15:40:12","slug":"development-and-evaluation-of-a-duo-sars-cov-2-rt-qpcr-assay-combining-two-assays-approved-by-the-world-health-organization-targeting-the-envelope-and-the-rna-dependant-rna-polymerase-rdrp-codi","status":"publish","type":"post","link":"https:\/\/depts.washington.edu\/pandemicalliance\/2020\/07\/08\/development-and-evaluation-of-a-duo-sars-cov-2-rt-qpcr-assay-combining-two-assays-approved-by-the-world-health-organization-targeting-the-envelope-and-the-rna-dependant-rna-polymerase-rdrp-codi\/","title":{"rendered":"Development and Evaluation of a Duo SARS-CoV-2 RT-QPCR Assay Combining Two Assays Approved by the World Health Organization Targeting the Envelope and the RNA-Dependant\u00a0RNA Polymerase (RdRp) Coding Regions"},"content":{"rendered":"<ul>\n<li data-leveltext=\"\uf0b7\" data-font=\"Symbol\" data-listid=\"34\" data-aria-posinset=\"1\" data-aria-level=\"1\"><span data-contrast=\"auto\">Pezzi et al. evaluated a duo SARS-CoV-2 RT-qPCR assay. Compared to\u00a0monoplex\u00a0RT-qPCR assays, dual-target assays may be less likely to have false-negative results caused by polymorphisms or point mutations. Based on 69 nasopharyngeal samples, the dual assay performed equal<\/span><span data-contrast=\"auto\">\u00a0to\u00a0<\/span><span data-contrast=\"auto\">or better<\/span><span data-contrast=\"auto\">\u00a0than\u00a0<\/span><span data-contrast=\"auto\">mono-target assay<\/span><span data-contrast=\"auto\">s<\/span><span data-contrast=\"auto\">\u00a0in terms of sensitivity, specificity, linearity<\/span><span data-contrast=\"auto\">,<\/span><span data-contrast=\"auto\">\u00a0and signal intensity.<\/span><span data-ccp-props=\"{&quot;134233279&quot;:true,&quot;201341983&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/li>\n<\/ul>\n<p><i><span data-contrast=\"none\">Pezzi et al. (June 25, 2020). Development and Evaluation of a Duo SARS-CoV-2 RT-QPCR Assay Combining Two Assays Approved by the World Health Organization Targeting the Envelope and the RNA-Dependant\u00a0RNA Polymerase (RdRp) Coding Regions. Viruses.\u00a0<\/span><\/i><a href=\"https:\/\/doi.org\/10.3390\/v12060686\"><span data-contrast=\"none\">https:\/\/doi.org\/10.3390\/v12060686<\/span><\/a><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559685&quot;:720,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Pezzi et al. evaluated a duo SARS-CoV-2 RT-qPCR assay. Compared to\u00a0monoplex\u00a0RT-qPCR assays, dual-target assays may be less likely to have false-negative results caused by polymorphisms or point mutations. Based on 69 nasopharyngeal samples, the dual assay performed equal\u00a0to\u00a0or better\u00a0than\u00a0mono-target assays\u00a0in terms of sensitivity, specificity, linearity,\u00a0and signal intensity.\u00a0 Pezzi et al. (June 25, 2020). Development and&#8230;<\/p>\n<div><a class=\"more\" href=\"https:\/\/depts.washington.edu\/pandemicalliance\/2020\/07\/08\/development-and-evaluation-of-a-duo-sars-cov-2-rt-qpcr-assay-combining-two-assays-approved-by-the-world-health-organization-targeting-the-envelope-and-the-rna-dependant-rna-polymerase-rdrp-codi\/\">Read more<\/a><\/div>\n","protected":false},"author":8,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":"","_links_to":"","_links_to_target":""},"categories":[6],"tags":[],"topic":[19],"class_list":["post-8037","post","type-post","status-publish","format-standard","hentry","category-article-summary","topic-testing-and-treatment"],"_links":{"self":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/8037","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/comments?post=8037"}],"version-history":[{"count":1,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/8037\/revisions"}],"predecessor-version":[{"id":8038,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/8037\/revisions\/8038"}],"wp:attachment":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/media?parent=8037"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/categories?post=8037"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/tags?post=8037"},{"taxonomy":"topic","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/topic?post=8037"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}