{"id":8165,"date":"2021-03-30T09:59:44","date_gmt":"2021-03-30T16:59:44","guid":{"rendered":"https:\/\/depts.washington.edu\/pandemicalliance\/?p=8165"},"modified":"2021-03-31T10:00:38","modified_gmt":"2021-03-31T17:00:38","slug":"sars-cov-2-variant-b-1-1-7-caused-hla-a2-cd8-t-cell-epitope-mutations-for-impaired-cellular-immune-response","status":"publish","type":"post","link":"https:\/\/depts.washington.edu\/pandemicalliance\/2021\/03\/30\/sars-cov-2-variant-b-1-1-7-caused-hla-a2-cd8-t-cell-epitope-mutations-for-impaired-cellular-immune-response\/","title":{"rendered":"SARS-CoV-2 Variant B.1.1.7 Caused HLA-A2+ CD8+ T Cell Epitope Mutations for Impaired Cellular Immune Response"},"content":{"rendered":"<ul>\n<li style=\"font-weight: 400\"><i><span style=\"font-weight: 400\">[Pre-print, not peer-reviewed]<\/span><\/i><span style=\"font-weight: 400\"> The SARS-CoV-2 B.1.1.7 variant is associated with reduced CD8+ T cell activation due to at least two specific mutations in ORF1. The authors used algorithms to predict HLA-A2 binding epitopes in both the B.1.1.7 strain and the ancestral Wuhan strain and determined whether these epitopes could activate CD8+ T cells using an artificial antigen presentation system. Two mutations located in non-structural proteins of the B.1.1.7 variant (A1708D mutation in ORF1ab1707-1716 and I2230T mutation in ORF1ab2230-2238) were linked in the decreased activation of CD8+ T cells. The authors then constructed SARS-CoV-2 CD8+ tetramers based upon these predicted epitopes and used them to probe the CD8+ T cell memory from convalescent patients. They found that most CD8+ T cells from convalescent patients had an effector memory phenotype and furthermore there was substantially reduced recognition of the B.1.1.7 mutant epitopes.<\/span><\/li>\n<\/ul>\n<p><i><span style=\"font-weight: 400\">Xiao et al.\u00a0(Mar 29, 2021). SARS-CoV-2 Variant B.1.1.7 Caused HLA-A2+ CD8+ T Cell Epitope Mutations for Impaired Cellular Immune Response. Pre-print downloaded Mar 30 from <\/span><\/i><a href=\"https:\/\/doi.org\/10.1101\/2021.03.28.437363\"><span style=\"font-weight: 400\">https:\/\/doi.org\/10.1101\/2021.03.28.437363<\/span><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>[Pre-print, not peer-reviewed] The SARS-CoV-2 B.1.1.7 variant is associated with reduced CD8+ T cell activation due to at least two specific mutations in ORF1. The authors used algorithms to predict HLA-A2 binding epitopes in both the B.1.1.7 strain and the ancestral Wuhan strain and determined whether these epitopes could activate CD8+ T cells using an&#8230;<\/p>\n<div><a class=\"more\" href=\"https:\/\/depts.washington.edu\/pandemicalliance\/2021\/03\/30\/sars-cov-2-variant-b-1-1-7-caused-hla-a2-cd8-t-cell-epitope-mutations-for-impaired-cellular-immune-response\/\">Read more<\/a><\/div>\n","protected":false},"author":8,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":"","_links_to":"","_links_to_target":""},"categories":[6],"tags":[32,159],"topic":[31],"class_list":["post-8165","post","type-post","status-publish","format-standard","hentry","category-article-summary","tag-immunity","tag-variants","topic-vaccines-and-immunity"],"_links":{"self":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/8165","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/comments?post=8165"}],"version-history":[{"count":1,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/8165\/revisions"}],"predecessor-version":[{"id":8166,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/8165\/revisions\/8166"}],"wp:attachment":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/media?parent=8165"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/categories?post=8165"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/tags?post=8165"},{"taxonomy":"topic","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/topic?post=8165"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}