{"id":8609,"date":"2021-04-02T09:49:07","date_gmt":"2021-04-02T16:49:07","guid":{"rendered":"https:\/\/depts.washington.edu\/pandemicalliance\/?p=8609"},"modified":"2021-04-06T09:49:50","modified_gmt":"2021-04-06T16:49:50","slug":"a-method-for-detection-of-sars-cov-2-rna-in-healthy-human-stool-a-validation-study","status":"publish","type":"post","link":"https:\/\/depts.washington.edu\/pandemicalliance\/2021\/04\/02\/a-method-for-detection-of-sars-cov-2-rna-in-healthy-human-stool-a-validation-study\/","title":{"rendered":"A Method for Detection of SARS-CoV-2 RNA in Healthy Human Stool: A Validation Study"},"content":{"rendered":"<ul>\n<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">A modified RT-PCR test to detect SARS-CoV-2 RNA in human stool had 100% analytical sensitivity in 20 stool samples freshly spiked with inactivated SARS-CoV-2 material and a lower limit of detection (LLD) of 3,000 viral RNA copies per gram. Analytical sensitivity decreased two-fold after a single freeze-thaw cycle at -80 \u00b0C. The assay had 100% sensitivity and specificity when used by a different laboratory to correctly identify 30 stool samples for the presence of SARS-CoV-2. Additionally, SARS-CoV-2 RNA was detected using this assay in the stool and anal swab specimens of 11 of 23 positive individuals.<\/span><\/li>\n<\/ul>\n<p><i><span style=\"font-weight: 400\">Coryell et al.\u00a0(Mar 31, 2021). A Method for Detection of SARS-CoV-2 RNA in Healthy Human Stool: A Validation Study. The Lancet Microbe. <\/span><\/i><a href=\"https:\/\/doi.org\/10.1016\/S2666-5247(21)00059-8\"><span style=\"font-weight: 400\">https:\/\/doi.org\/10.1016\/S2666-5247(21)00059-8<\/span><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>A modified RT-PCR test to detect SARS-CoV-2 RNA in human stool had 100% analytical sensitivity in 20 stool samples freshly spiked with inactivated SARS-CoV-2 material and a lower limit of detection (LLD) of 3,000 viral RNA copies per gram. Analytical sensitivity decreased two-fold after a single freeze-thaw cycle at -80 \u00b0C. The assay had 100%&#8230;<\/p>\n<div><a class=\"more\" href=\"https:\/\/depts.washington.edu\/pandemicalliance\/2021\/04\/02\/a-method-for-detection-of-sars-cov-2-rna-in-healthy-human-stool-a-validation-study\/\">Read more<\/a><\/div>\n","protected":false},"author":8,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":"","_links_to":"","_links_to_target":""},"categories":[6],"tags":[27],"topic":[19],"class_list":["post-8609","post","type-post","status-publish","format-standard","hentry","category-article-summary","tag-testing","topic-testing-and-treatment"],"_links":{"self":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/8609","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/comments?post=8609"}],"version-history":[{"count":1,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/8609\/revisions"}],"predecessor-version":[{"id":8610,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/8609\/revisions\/8610"}],"wp:attachment":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/media?parent=8609"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/categories?post=8609"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/tags?post=8609"},{"taxonomy":"topic","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/topic?post=8609"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}