{"id":8664,"date":"2020-07-31T10:30:00","date_gmt":"2020-07-31T17:30:00","guid":{"rendered":"https:\/\/depts.washington.edu\/pandemicalliance\/?p=8664"},"modified":"2021-04-06T10:30:40","modified_gmt":"2021-04-06T17:30:40","slug":"pooled-rna-sample-reverse-transcriptase-real-time-pcr-assay-for-sars-cov-2-infection-a-reliable-faster-and-economical-method","status":"publish","type":"post","link":"https:\/\/depts.washington.edu\/pandemicalliance\/2020\/07\/31\/pooled-rna-sample-reverse-transcriptase-real-time-pcr-assay-for-sars-cov-2-infection-a-reliable-faster-and-economical-method\/","title":{"rendered":"Pooled RNA Sample Reverse Transcriptase Real Time PCR Assay for SARS CoV-2 Infection: A Reliable, Faster and Economical Method"},"content":{"rendered":"<ul>\n<li data-leveltext=\"\uf0b7\" data-font=\"Symbol\" data-listid=\"16\" data-aria-posinset=\"1\" data-aria-level=\"1\"><span data-contrast=\"auto\">Gupta et al. demonstrate that pool testing with 8 RNA samples analyzed with RT-qPCR can achieve a sensitivity of 95.4%, a specificity of 100%<\/span><span data-contrast=\"auto\">\u00a0(<\/span><span data-contrast=\"auto\">280 samples\u00a0<\/span><span data-contrast=\"auto\">tested<\/span><span data-contrast=\"auto\">:\u00a0<\/span><span data-contrast=\"auto\">40 positive and 240 negative<\/span><span data-contrast=\"auto\">).<\/span><span data-ccp-props=\"{&quot;134233279&quot;:true,&quot;201341983&quot;:0,&quot;335559685&quot;:360,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/li>\n<li data-leveltext=\"\uf0b7\" data-font=\"Symbol\" data-listid=\"16\" data-aria-posinset=\"2\" data-aria-level=\"1\"><span data-contrast=\"auto\">The authors\u00a0<\/span><span data-contrast=\"auto\">conclude that the results of pool testing are not affected by the number of positive samples in a pool, suggesting the utility of pooled testing\u00a0<\/span><span data-contrast=\"auto\">at various stages of the pandemic.<\/span><span data-ccp-props=\"{&quot;134233279&quot;:true,&quot;201341983&quot;:0,&quot;335559685&quot;:360,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/li>\n<\/ul>\n<p><i><span data-contrast=\"none\">Gupta et al. (July 30, 2020). Pooled RNA Sample Reverse Transcriptase Real Time PCR Assay for SARS CoV-2 Infection: A Reliable, Faster and Economical Method. PLOS ONE.\u00a0<\/span><\/i><a href=\"https:\/\/doi.org\/10.1371\/journal.pone.0236859\"><span data-contrast=\"none\">https:\/\/doi.org\/10.1371\/journal.pone.0236859<\/span><\/a><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559685&quot;:720,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Gupta et al. demonstrate that pool testing with 8 RNA samples analyzed with RT-qPCR can achieve a sensitivity of 95.4%, a specificity of 100%\u00a0(280 samples\u00a0tested:\u00a040 positive and 240 negative).\u00a0 The authors\u00a0conclude that the results of pool testing are not affected by the number of positive samples in a pool, suggesting the utility of pooled testing\u00a0at&#8230;<\/p>\n<div><a class=\"more\" href=\"https:\/\/depts.washington.edu\/pandemicalliance\/2020\/07\/31\/pooled-rna-sample-reverse-transcriptase-real-time-pcr-assay-for-sars-cov-2-infection-a-reliable-faster-and-economical-method\/\">Read more<\/a><\/div>\n","protected":false},"author":8,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":"","_links_to":"","_links_to_target":""},"categories":[6],"tags":[],"topic":[19],"class_list":["post-8664","post","type-post","status-publish","format-standard","hentry","category-article-summary","topic-testing-and-treatment"],"_links":{"self":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/8664","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/comments?post=8664"}],"version-history":[{"count":1,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/8664\/revisions"}],"predecessor-version":[{"id":8665,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/8664\/revisions\/8665"}],"wp:attachment":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/media?parent=8664"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/categories?post=8664"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/tags?post=8664"},{"taxonomy":"topic","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/topic?post=8664"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}