{"id":8717,"date":"2020-08-04T11:05:20","date_gmt":"2020-08-04T18:05:20","guid":{"rendered":"https:\/\/depts.washington.edu\/pandemicalliance\/?p=8717"},"modified":"2021-04-06T11:06:08","modified_gmt":"2021-04-06T18:06:08","slug":"antibody-response-against-sars-cov-2-spike-protein-and-nucleoprotein-evaluated-by-4-automated-immunoassays-and-3-elisas","status":"publish","type":"post","link":"https:\/\/depts.washington.edu\/pandemicalliance\/2020\/08\/04\/antibody-response-against-sars-cov-2-spike-protein-and-nucleoprotein-evaluated-by-4-automated-immunoassays-and-3-elisas\/","title":{"rendered":"Antibody Response against SARS-CoV-2 Spike Protein and Nucleoprotein Evaluated by 4 Automated Immunoassays and 3 ELISAs"},"content":{"rendered":"<ul>\n<li data-leveltext=\"\uf0b7\" data-font=\"Symbol\" data-listid=\"45\" data-aria-posinset=\"1\" data-aria-level=\"1\"><span data-contrast=\"auto\">To measure sensitivity of antibody response against SARS-CoV-2 spike protein and nucleoprotein,\u00a0<\/span><span data-contrast=\"auto\">4\u00a0<\/span><span data-contrast=\"auto\">automated immunoassays\u00a0<\/span><span data-contrast=\"auto\">(Roche Abbott,\u00a0<\/span><span data-contrast=\"auto\">Diasorin<\/span><span data-contrast=\"auto\">, and\u00a0<\/span><span data-contrast=\"auto\">Snibe<\/span><span data-contrast=\"auto\">)\u00a0<\/span><span data-contrast=\"auto\">and 3 ELISA<\/span><span data-contrast=\"auto\">s\u00a0<\/span><span data-contrast=\"auto\">(2 from\u00a0<\/span><span data-contrast=\"auto\">Euroimmun<\/span><span data-contrast=\"auto\">\u00a0and\u00a0<\/span><span data-contrast=\"auto\">Mikrogen<\/span><span data-contrast=\"auto\">) were evaluated<\/span><span data-contrast=\"auto\">\u00a0using\u00a0<\/span><span data-contrast=\"auto\">samples from 114 patients with moderate, severe, or critical COVID-19\u00a0<\/span><span data-contrast=\"auto\">and\u00a0<\/span><span data-contrast=\"auto\">113 pre-pandemic samples.\u00a0<\/span><span data-contrast=\"auto\">T<\/span><span data-contrast=\"auto\">hree weeks\u00a0<\/span><span data-contrast=\"auto\">post-symptom\u00a0<\/span><span data-contrast=\"auto\">onset<\/span><span data-contrast=\"auto\">,\u00a0<\/span><span data-contrast=\"auto\">sensitivity was 100%. Specificity varied from 94.7% to 100%; using a ROC curve to estimate cut-offs corresponding to specificities of 95% and 97.5%,\u00a0<\/span><span data-contrast=\"auto\">the\u00a0<\/span><span data-contrast=\"auto\">Roche\u00a0<\/span><span data-contrast=\"auto\">assay (all immunoglobulins, anti-nucleocapsid)\u00a0<\/span><span data-contrast=\"auto\">had the highest sensitivity (85% and 81%, respectively). Seroconversion occurred on average 2 days earlier for anti-nuc<\/span><span data-contrast=\"auto\">l<\/span><span data-contrast=\"auto\">eoca<\/span><span data-contrast=\"auto\">p<\/span><span data-contrast=\"auto\">sid assays than the anti-spike assays.\u00a0<\/span><span data-contrast=\"auto\">T<\/span><span data-contrast=\"auto\">ime to\u00a0<\/span><span data-contrast=\"auto\">IgG\u00a0<\/span><span data-contrast=\"auto\">seroconversion\u00a0<\/span><span data-contrast=\"auto\">was similar\u00a0<\/span><span data-contrast=\"auto\">for critical\u00a0<\/span><span data-contrast=\"auto\">and<\/span><span data-contrast=\"auto\">\u00a0non-critical cases.\u00a0\u00a0<\/span><span data-ccp-props=\"{&quot;134233279&quot;:true,&quot;201341983&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:240}\">\u00a0<\/span><\/li>\n<\/ul>\n<p><i><span data-contrast=\"auto\">Van\u00a0<\/span><\/i><i><span data-contrast=\"auto\">Elslande<\/span><\/i><i><span data-contrast=\"auto\">\u00a0et al. (July 2020). Antibody Response against SARS-CoV-2 Spike Protein and Nucleoprotein Evaluated by 4 Automated Immunoassays and 3 ELISAs. Clinical Microbiology and Infection.\u00a0<\/span><\/i><a href=\"https:\/\/doi.org\/10.1016\/j.cmi.2020.07.038\"><i><span data-contrast=\"none\">https:\/\/doi.org\/10.1016\/j.cmi.2020.07.038<\/span><\/i><\/a><i><span data-contrast=\"auto\">\u00a0<\/span><\/i><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559685&quot;:720,&quot;335559739&quot;:160,&quot;335559740&quot;:240}\">\u00a0<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p>To measure sensitivity of antibody response against SARS-CoV-2 spike protein and nucleoprotein,\u00a04\u00a0automated immunoassays\u00a0(Roche Abbott,\u00a0Diasorin, and\u00a0Snibe)\u00a0and 3 ELISAs\u00a0(2 from\u00a0Euroimmun\u00a0and\u00a0Mikrogen) were evaluated\u00a0using\u00a0samples from 114 patients with moderate, severe, or critical COVID-19\u00a0and\u00a0113 pre-pandemic samples.\u00a0Three weeks\u00a0post-symptom\u00a0onset,\u00a0sensitivity was 100%. Specificity varied from 94.7% to 100%; using a ROC curve to estimate cut-offs corresponding to specificities of 95% and 97.5%,\u00a0the\u00a0Roche\u00a0assay (all&#8230;<\/p>\n<div><a class=\"more\" href=\"https:\/\/depts.washington.edu\/pandemicalliance\/2020\/08\/04\/antibody-response-against-sars-cov-2-spike-protein-and-nucleoprotein-evaluated-by-4-automated-immunoassays-and-3-elisas\/\">Read more<\/a><\/div>\n","protected":false},"author":8,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":"","_links_to":"","_links_to_target":""},"categories":[6],"tags":[],"topic":[19],"class_list":["post-8717","post","type-post","status-publish","format-standard","hentry","category-article-summary","topic-testing-and-treatment"],"_links":{"self":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/8717","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/comments?post=8717"}],"version-history":[{"count":1,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/8717\/revisions"}],"predecessor-version":[{"id":8718,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/8717\/revisions\/8718"}],"wp:attachment":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/media?parent=8717"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/categories?post=8717"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/tags?post=8717"},{"taxonomy":"topic","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/topic?post=8717"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}