{"id":8738,"date":"2020-08-05T11:17:15","date_gmt":"2020-08-05T18:17:15","guid":{"rendered":"https:\/\/depts.washington.edu\/pandemicalliance\/?p=8738"},"modified":"2021-04-06T11:17:57","modified_gmt":"2021-04-06T18:17:57","slug":"challenges-in-use-of-saliva-for-detection-of-sars-cov-2-rna-in-symptomatic-outpatients","status":"publish","type":"post","link":"https:\/\/depts.washington.edu\/pandemicalliance\/2020\/08\/05\/challenges-in-use-of-saliva-for-detection-of-sars-cov-2-rna-in-symptomatic-outpatients\/","title":{"rendered":"Challenges in Use of Saliva for Detection of SARS CoV-2 RNA in Symptomatic Outpatients"},"content":{"rendered":"<ul>\n<li data-leveltext=\"\uf0b7\" data-font=\"Symbol\" data-listid=\"8\" data-aria-posinset=\"2\" data-aria-level=\"1\"><span data-contrast=\"auto\">Landry et al.<\/span><span data-contrast=\"auto\">\u00a0<\/span><span data-contrast=\"auto\">found<\/span><span data-contrast=\"auto\">\u00a0that real-time RT-PCR of pure saliva samples had an overall sensitivity for SARS CoV-2 RNA detection of 85.7 % when compared to simultaneously collected nasopharyngeal swab samples.<\/span><span data-ccp-props=\"{&quot;134233279&quot;:true,&quot;201341983&quot;:0,&quot;335559739&quot;:120,&quot;335559740&quot;:259}\">\u00a0<\/span><\/li>\n<\/ul>\n<ul>\n<li data-leveltext=\"\uf0b7\" data-font=\"Symbol\" data-listid=\"8\" data-aria-posinset=\"1\" data-aria-level=\"1\"><span data-contrast=\"auto\">More than 33% of the saliva samples were initially too thick or stringy to be pipetted efficiently, requiring an additional sample preparation step before testing. The additional step slowed processing significantly and increased the risk of work-station contamination. Saliva samples requiring this additional step may not be appropriate for high volume testing, which requires ready aspiration by robotic instruments.\u00a0<\/span><span data-ccp-props=\"{&quot;134233279&quot;:true,&quot;201341983&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/li>\n<\/ul>\n<p><i><span data-contrast=\"none\">Landry et al. (<\/span><\/i><i><span data-contrast=\"none\">July 31,<\/span><\/i><i><span data-contrast=\"none\">\u00a02020). Challenges in Use of Saliva for Detection of SARS CoV-2 RNA in Symptomatic Outpatients. Journal of Clinical Virology.\u00a0<\/span><\/i><a href=\"https:\/\/doi.org\/10.1016\/j.jcv.2020.104567\"><span data-contrast=\"none\">https:\/\/doi.org\/10.1016\/j.jcv.2020.104567<\/span><\/a><i><span data-contrast=\"none\">\u00a0\u00a0<\/span><\/i><i><span data-contrast=\"none\">\u00a0\u00a0<\/span><\/i><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559685&quot;:720,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Landry et al.\u00a0found\u00a0that real-time RT-PCR of pure saliva samples had an overall sensitivity for SARS CoV-2 RNA detection of 85.7 % when compared to simultaneously collected nasopharyngeal swab samples.\u00a0 More than 33% of the saliva samples were initially too thick or stringy to be pipetted efficiently, requiring an additional sample preparation step before testing. The&#8230;<\/p>\n<div><a class=\"more\" href=\"https:\/\/depts.washington.edu\/pandemicalliance\/2020\/08\/05\/challenges-in-use-of-saliva-for-detection-of-sars-cov-2-rna-in-symptomatic-outpatients\/\">Read more<\/a><\/div>\n","protected":false},"author":8,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":"","_links_to":"","_links_to_target":""},"categories":[6],"tags":[],"topic":[19],"class_list":["post-8738","post","type-post","status-publish","format-standard","hentry","category-article-summary","topic-testing-and-treatment"],"_links":{"self":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/8738","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/comments?post=8738"}],"version-history":[{"count":1,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/8738\/revisions"}],"predecessor-version":[{"id":8739,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/8738\/revisions\/8739"}],"wp:attachment":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/media?parent=8738"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/categories?post=8738"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/tags?post=8738"},{"taxonomy":"topic","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/topic?post=8738"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}