{"id":9355,"date":"2021-04-22T15:38:55","date_gmt":"2021-04-22T22:38:55","guid":{"rendered":"https:\/\/depts.washington.edu\/pandemicalliance\/?p=9355"},"modified":"2021-04-23T15:39:34","modified_gmt":"2021-04-23T22:39:34","slug":"an-international-interlaboratory-ring-trial-confirms-the-feasibility-of-an-open-source-extraction-less-direct-rt-qpcr-method-for-reliable-detection-of-sars-cov-2-rna-in-clinical-sa","status":"publish","type":"post","link":"https:\/\/depts.washington.edu\/pandemicalliance\/2021\/04\/22\/an-international-interlaboratory-ring-trial-confirms-the-feasibility-of-an-open-source-extraction-less-direct-rt-qpcr-method-for-reliable-detection-of-sars-cov-2-rna-in-clinical-sa\/","title":{"rendered":"An International, Interlaboratory Ring Trial Confirms the Feasibility of an Open-Source, Extraction-Less \u201cDirect\u201d RT-QPCR Method for Reliable Detection of SARS-CoV-2 RNA in Clinical Samples"},"content":{"rendered":"<ul>\n<li style=\"font-weight: 400\"><i><span style=\"font-weight: 400\">[Pre-print, not peer-reviewed]<\/span><\/i><span style=\"font-weight: 400\"> An international interlaboratory trial observed 100% concordance in using &#8220;direct&#8221; RT-PCR across the 10 included laboratories (3 in US, including the University of Washington Virology Lab [UWVL]). &#8220;Direct&#8221; or \u201cextraction-less\u201d RT-PCR is an inexpensive alternative to conventional RT-qPCR that does not require a resource intensive RNA extraction step. The assay was found to have 99.6% sensitivity and 100% specificity across participating laboratories in discerning 5 negative and 25 positive samples prepared by the UWVL. There was high intralaboratory correlation for cycle threshold (Ct value), including samples with low concentrations of virus (high Ct values).<\/span><\/li>\n<\/ul>\n<p><i><span style=\"font-weight: 400\">Mills et al.\u00a0(Apr 16, 2021). An International, Interlaboratory Ring Trial Confirms the Feasibility of an Open-Source, Extraction-Less \u201cDirect\u201d RT-QPCR Method for Reliable Detection of SARS-CoV-2 RNA in Clinical Samples. Pre-print downloaded Apr 22 from <\/span><\/i><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/33880478\/\"><span style=\"font-weight: 400\">https:\/\/pubmed.ncbi.nlm.nih.gov\/33880478\/<\/span><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>[Pre-print, not peer-reviewed] An international interlaboratory trial observed 100% concordance in using &#8220;direct&#8221; RT-PCR across the 10 included laboratories (3 in US, including the University of Washington Virology Lab [UWVL]). &#8220;Direct&#8221; or \u201cextraction-less\u201d RT-PCR is an inexpensive alternative to conventional RT-qPCR that does not require a resource intensive RNA extraction step. The assay was found&#8230;<\/p>\n<div><a class=\"more\" href=\"https:\/\/depts.washington.edu\/pandemicalliance\/2021\/04\/22\/an-international-interlaboratory-ring-trial-confirms-the-feasibility-of-an-open-source-extraction-less-direct-rt-qpcr-method-for-reliable-detection-of-sars-cov-2-rna-in-clinical-sa\/\">Read more<\/a><\/div>\n","protected":false},"author":8,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":"","_links_to":"","_links_to_target":""},"categories":[6],"tags":[27],"topic":[19],"class_list":["post-9355","post","type-post","status-publish","format-standard","hentry","category-article-summary","tag-testing","topic-testing-and-treatment"],"_links":{"self":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/9355","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/comments?post=9355"}],"version-history":[{"count":1,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/9355\/revisions"}],"predecessor-version":[{"id":9356,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/posts\/9355\/revisions\/9356"}],"wp:attachment":[{"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/media?parent=9355"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/categories?post=9355"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/tags?post=9355"},{"taxonomy":"topic","embeddable":true,"href":"https:\/\/depts.washington.edu\/pandemicalliance\/wp-json\/wp\/v2\/topic?post=9355"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}