{"id":18987,"date":"2025-10-08T16:53:20","date_gmt":"2025-10-08T23:53:20","guid":{"rendered":"https:\/\/depts.washington.edu\/pnwcesu\/?p=18987"},"modified":"2025-10-09T10:07:08","modified_gmt":"2025-10-09T17:07:08","slug":"project-highlight-pygmy-rabbits-in-idaho-using-genetic-analyses-to-estimate-occupancy","status":"publish","type":"post","link":"https:\/\/depts.washington.edu\/pnwcesu\/project-highlight-pygmy-rabbits-in-idaho-using-genetic-analyses-to-estimate-occupancy\/","title":{"rendered":"Project Highlight: Pygmy Rabbits in Idaho &#8211; Using Genetic Analyses to Estimate Occupancy"},"content":{"rendered":"<h4 class=\"wp-block-heading\">By Kylie Baker<\/h4>\n<h3 class=\"wp-block-heading\">Mapping the Hidden World of Pygmy Rabbits: Genetic Tools and Collaborative Surveys in Idaho\u2019s Sagebrush Steppe<\/h3>\n<p>In the arid expanses of the sagebrush steppe, an elusive burrower quietly reshapes the landscape in profound ways: the pygmy rabbit. As the smallest rabbit species in the world, pygmy rabbits (<em>Brachylagus idahoensis<\/em>) are sagebrush obligates, meaning they rely on this plant for both food and shelter. Notably, they\u2019re one of the very few vertebrate species able to subsist almost entirely on sagebrush, a plant rich in toxic compounds designed to deter browsing. This makes them a biological anomaly and an essential indicator of sagebrush ecosystem health.&nbsp;<\/p>\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"768\" src=\"https:\/\/depts.washington.edu\/pnwcesu\/wordpress\/wp-content\/uploads\/2025\/10\/pygmy_winter1-1024x768.jpg\" alt=\"\" class=\"wp-image-18988\" srcset=\"https:\/\/depts.washington.edu\/pnwcesu\/wordpress\/wp-content\/uploads\/2025\/10\/pygmy_winter1-1024x768.jpg 1024w, https:\/\/depts.washington.edu\/pnwcesu\/wordpress\/wp-content\/uploads\/2025\/10\/pygmy_winter1-300x225.jpg 300w, https:\/\/depts.washington.edu\/pnwcesu\/wordpress\/wp-content\/uploads\/2025\/10\/pygmy_winter1-768x576.jpg 768w, https:\/\/depts.washington.edu\/pnwcesu\/wordpress\/wp-content\/uploads\/2025\/10\/pygmy_winter1-375x281.jpg 375w, https:\/\/depts.washington.edu\/pnwcesu\/wordpress\/wp-content\/uploads\/2025\/10\/pygmy_winter1-750x563.jpg 750w, https:\/\/depts.washington.edu\/pnwcesu\/wordpress\/wp-content\/uploads\/2025\/10\/pygmy_winter1-1140x855.jpg 1140w, https:\/\/depts.washington.edu\/pnwcesu\/wordpress\/wp-content\/uploads\/2025\/10\/pygmy_winter1-108x81.jpg 108w, https:\/\/depts.washington.edu\/pnwcesu\/wordpress\/wp-content\/uploads\/2025\/10\/pygmy_winter1.jpg 1152w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><figcaption class=\"wp-element-caption\"><strong>Pygmy rabbit in the field. <\/strong><em>Credit: Rachlow.<\/em><\/figcaption><\/figure>\n<\/div>\n<div class=\"wp-block-media-text is-stacked-on-mobile\">\n<figure class=\"wp-block-media-text__media\"><img loading=\"lazy\" decoding=\"async\" width=\"960\" height=\"684\" src=\"https:\/\/depts.washington.edu\/pnwcesu\/wordpress\/wp-content\/uploads\/2025\/10\/Project-Highlight-Visuals-9.jpg\" alt=\"\" class=\"wp-image-18999 size-full\" srcset=\"https:\/\/depts.washington.edu\/pnwcesu\/wordpress\/wp-content\/uploads\/2025\/10\/Project-Highlight-Visuals-9.jpg 960w, https:\/\/depts.washington.edu\/pnwcesu\/wordpress\/wp-content\/uploads\/2025\/10\/Project-Highlight-Visuals-9-300x214.jpg 300w, https:\/\/depts.washington.edu\/pnwcesu\/wordpress\/wp-content\/uploads\/2025\/10\/Project-Highlight-Visuals-9-768x547.jpg 768w, https:\/\/depts.washington.edu\/pnwcesu\/wordpress\/wp-content\/uploads\/2025\/10\/Project-Highlight-Visuals-9-375x267.jpg 375w, https:\/\/depts.washington.edu\/pnwcesu\/wordpress\/wp-content\/uploads\/2025\/10\/Project-Highlight-Visuals-9-750x534.jpg 750w\" sizes=\"auto, (max-width: 960px) 100vw, 960px\" \/><\/figure>\n<div class=\"wp-block-media-text__content\">\n<p>Many do not appreciate the importance of pygmy rabbits. Sagebrush habitat conservation largely centers on the sage-grouse, leaving little attention or credit to the powerful pygmies. However, pygmy rabbits are considered ecosystem engineers; they play a vital role in the sagebrush steppe ecosystem. Their burrowing activity aerates soil, improves water infiltration, and enriches the soil with nutrients\u2014all of which boost sagebrush reproduction. Their burrows create microhabitats that support a range of other species, from birds to insects. They\u2019re inconspicuous, but their impact is profound.<\/p>\n<\/div>\n<\/div>\n<p>Despite their ecological significance, pygmy rabbits are rarely seen and are notoriously difficult to study, making it a challenge for scientists and land managers to understand where they persist, how populations are changing, and how best to protect them. A Pacific Northwest Cooperative Ecosystem Studies Unit (PNW CESU) multi-partner collaboration led by Dr. Janet Rachlow at the University of Idaho and supported by the U.S. Fish and Wildlife Service addresses these knowledge gaps. Their project combines traditional field surveys with genetic analysis to better map pygmy rabbit occupancy across Idaho and parts of Montana. This new effort builds on a prior Bureau of Land Management-funded project focused on refining maps of priority pygmy rabbit habitat. With a renewed potential listing for pygmy rabbits under the Endangered Species Act (ESA), it is crucial now more than ever for researchers to have a baseline of pygmy rabbit occupancy to determine any population changes.&nbsp;<\/p>\n<h3 class=\"wp-block-heading\">Improving Survey Accuracy Through Occupancy Modeling and DNA Analysis<\/h3>\n<p>Previous surveys of pygmy rabbits often relied on field observations of burrow systems and pellet identification, with the assumption that if no evidence was found, the rabbits weren\u2019t present. But as Dr. Rachlow noted, \u201cThey\u2019re wily. They may be there and go undetected.\u201d Occupancy modeling offers a solution. This statistical approach accounts for the possibility of false absences by calculating the probability of detection, giving researchers a much clearer picture of where pygmy rabbits are actually present, even when signs are sparse.<\/p>\n<div class=\"wp-block-media-text has-media-on-the-right is-stacked-on-mobile\">\n<div class=\"wp-block-media-text__content\">\n<p>To ensure accurate species identification, the team is supplementing field observations with genetic confirmation. Pygmy rabbit pellets\u2014collected from snow-covered burrow sites during winter surveys\u2014are analyzed in the lab to confirm species. This is crucial because juvenile cottontails, which overlap with pygmy rabbits in some areas, leave behind similarly sized pellets and can easily be mistaken in the field.<\/p>\n<\/div>\n<figure class=\"wp-block-media-text__media\"><img loading=\"lazy\" decoding=\"async\" width=\"481\" height=\"681\" src=\"https:\/\/depts.washington.edu\/pnwcesu\/wordpress\/wp-content\/uploads\/2025\/10\/Project-Highlight-Visuals-2.jpg\" alt=\"\" class=\"wp-image-18989 size-full\" srcset=\"https:\/\/depts.washington.edu\/pnwcesu\/wordpress\/wp-content\/uploads\/2025\/10\/Project-Highlight-Visuals-2.jpg 481w, https:\/\/depts.washington.edu\/pnwcesu\/wordpress\/wp-content\/uploads\/2025\/10\/Project-Highlight-Visuals-2-212x300.jpg 212w, https:\/\/depts.washington.edu\/pnwcesu\/wordpress\/wp-content\/uploads\/2025\/10\/Project-Highlight-Visuals-2-375x531.jpg 375w\" sizes=\"auto, (max-width: 481px) 100vw, 481px\" \/><\/figure>\n<\/div>\n<p>Support from the U.S. Fish and Wildlife Service funds this genetic work, strengthening the validity of survey results and providing a clear foundation for habitat modeling. Together, these tools allow for a far more accurate picture of pygmy rabbit occupancy across their range.<\/p>\n<h3 class=\"wp-block-heading\">A Rare, Large-Scale Collaboration<\/h3>\n<div class=\"wp-block-media-text is-stacked-on-mobile\">\n<figure class=\"wp-block-media-text__media\"><img loading=\"lazy\" decoding=\"async\" width=\"852\" height=\"684\" src=\"https:\/\/depts.washington.edu\/pnwcesu\/wordpress\/wp-content\/uploads\/2025\/10\/Project-Highlight-Visuals-10.jpg\" alt=\"\" class=\"wp-image-19001 size-full\" srcset=\"https:\/\/depts.washington.edu\/pnwcesu\/wordpress\/wp-content\/uploads\/2025\/10\/Project-Highlight-Visuals-10.jpg 852w, https:\/\/depts.washington.edu\/pnwcesu\/wordpress\/wp-content\/uploads\/2025\/10\/Project-Highlight-Visuals-10-300x241.jpg 300w, https:\/\/depts.washington.edu\/pnwcesu\/wordpress\/wp-content\/uploads\/2025\/10\/Project-Highlight-Visuals-10-768x617.jpg 768w, https:\/\/depts.washington.edu\/pnwcesu\/wordpress\/wp-content\/uploads\/2025\/10\/Project-Highlight-Visuals-10-375x301.jpg 375w, https:\/\/depts.washington.edu\/pnwcesu\/wordpress\/wp-content\/uploads\/2025\/10\/Project-Highlight-Visuals-10-750x602.jpg 750w\" sizes=\"auto, (max-width: 852px) 100vw, 852px\" \/><\/figure>\n<div class=\"wp-block-media-text__content\">\n<p>The broader survey effort has mobilized more than 100 biologists from federal and state agencies across Idaho and neighboring Montana, an impressively large and coordinated undertaking for a single small mammal species. \u201cIt was a huge collaborative effort,\u201d said Rachlow. Her PhD student, Fiona McKibben, led the logistical coordination, developing an internal website to streamline data collection, share training materials, and assign survey routes.<\/p>\n<\/div>\n<\/div>\n<p>Since the pygmy rabbit habitat spans across borders, the joint effort from biologists at the Idaho Department of Fish &amp; Game and the Montana Department of Fish, Wildlife &amp; Parks was critical. This collaboration ensures consistency and a unified regional approach to data collection. It is especially valuable now that pygmy rabbits are under renewed consideration for listing under the ESA.<\/p>\n<div class=\"wp-block-media-text has-media-on-the-right is-stacked-on-mobile\">\n<div class=\"wp-block-media-text__content\">\n<p>The scale of this effort is striking in its own right. Mobilizing this many field biologists across such a vast and rugged landscape speaks not only to the technical demands of pygmy rabbit detection but also to the growing recognition of the species\u2019 importance as a marker for sagebrush ecosystem health. Few wildlife projects of this scope can claim such a wide and united field network, let alone for a rabbit most people will never see.<\/p>\n<\/div>\n<figure class=\"wp-block-media-text__media\"><img loading=\"lazy\" decoding=\"async\" width=\"867\" height=\"720\" src=\"https:\/\/depts.washington.edu\/pnwcesu\/wordpress\/wp-content\/uploads\/2025\/10\/Project-Highlight-Visuals-11.jpg\" alt=\"\" class=\"wp-image-19002 size-full\" srcset=\"https:\/\/depts.washington.edu\/pnwcesu\/wordpress\/wp-content\/uploads\/2025\/10\/Project-Highlight-Visuals-11.jpg 867w, https:\/\/depts.washington.edu\/pnwcesu\/wordpress\/wp-content\/uploads\/2025\/10\/Project-Highlight-Visuals-11-300x249.jpg 300w, https:\/\/depts.washington.edu\/pnwcesu\/wordpress\/wp-content\/uploads\/2025\/10\/Project-Highlight-Visuals-11-768x638.jpg 768w, https:\/\/depts.washington.edu\/pnwcesu\/wordpress\/wp-content\/uploads\/2025\/10\/Project-Highlight-Visuals-11-375x311.jpg 375w, https:\/\/depts.washington.edu\/pnwcesu\/wordpress\/wp-content\/uploads\/2025\/10\/Project-Highlight-Visuals-11-750x623.jpg 750w\" sizes=\"auto, (max-width: 867px) 100vw, 867px\" \/><\/figure>\n<\/div>\n<h3 class=\"wp-block-heading\">Early Results<\/h3>\n<p>Over the course of two winters, survey teams visited more than 500 sites across Idaho and adjacent portions of Montana, marking one of the most extensive pygmy rabbit survey efforts to date. Of those sites, 197 were identified in the field as having pygmy rabbit presence. To date, 131 fecal samples have undergone genetic testing, with approximately 70% confirmed as pygmy rabbits and about 30% misidentified as cottontails. This greatly underscores the importance of DNA confirmation in occupancy work, especially when data are collected from a variety of locations, researchers, and dates.&nbsp;<\/p>\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"878\" src=\"https:\/\/depts.washington.edu\/pnwcesu\/wordpress\/wp-content\/uploads\/2025\/10\/survey_status-1024x878.jpg\" alt=\"\" class=\"wp-image-18990\" srcset=\"https:\/\/depts.washington.edu\/pnwcesu\/wordpress\/wp-content\/uploads\/2025\/10\/survey_status-1024x878.jpg 1024w, https:\/\/depts.washington.edu\/pnwcesu\/wordpress\/wp-content\/uploads\/2025\/10\/survey_status-300x257.jpg 300w, https:\/\/depts.washington.edu\/pnwcesu\/wordpress\/wp-content\/uploads\/2025\/10\/survey_status-768x658.jpg 768w, https:\/\/depts.washington.edu\/pnwcesu\/wordpress\/wp-content\/uploads\/2025\/10\/survey_status-1536x1317.jpg 1536w, https:\/\/depts.washington.edu\/pnwcesu\/wordpress\/wp-content\/uploads\/2025\/10\/survey_status-2048x1755.jpg 2048w, https:\/\/depts.washington.edu\/pnwcesu\/wordpress\/wp-content\/uploads\/2025\/10\/survey_status-375x321.jpg 375w, https:\/\/depts.washington.edu\/pnwcesu\/wordpress\/wp-content\/uploads\/2025\/10\/survey_status-750x643.jpg 750w, https:\/\/depts.washington.edu\/pnwcesu\/wordpress\/wp-content\/uploads\/2025\/10\/survey_status-1140x977.jpg 1140w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><figcaption class=\"wp-element-caption\"><strong>Map 1. <\/strong>Locations of field-identified pygmy rabbit sites, genetically confirmed sites, and sites with no detection. Note: Many field-identified sites are currently in the process of genetic testing for confirmation. <em>Credit: McKibben.<\/em><\/figcaption><\/figure>\n<\/div>\n<p>Fortunately, the team is having great success extracting viable DNA from the samples. Nearly all were usable, due in large part to optimal collection conditions\u2014fresh pellets on snow, kept frozen. These data are now being used to update occupancy models and refine range maps, which will inform ongoing conservation efforts.<\/p>\n<h3 class=\"wp-block-heading\">Why It Matters: Conservation Implications<\/h3>\n<p>This project has gained new urgency in light of a recent ESA listing petition, following a previous petition nearly 20 years ago. \u201cBack then, we didn\u2019t even have a baseline distribution map,\u201d said Rachlow. That changed in 2019 when her team published the first range-wide species distribution model, using available data from across the West. \u201cNow, we have a baseline to compare against,\u201d she added. \u201cWe can start to ask: are they increasing or decreasing, and why?\u201d<\/p>\n<p>Importantly, the data also allow land management agencies to make more informed decisions when planning roads, firebreaks, or energy infrastructure. The goal is not to halt development, but to do it smartly. Knowing where sensitive species occur allows you to avoid those areas or take appropriate precautions. It\u2019s about maintaining intact, functioning landscapes, where humans and wildlife can co-exist.<\/p>\n<h3 class=\"wp-block-heading\">Next Steps: Looking Ahead with Climate Projections and Trend Analysis<\/h3>\n<p>Looking forward, the research team plans to use these data to evaluate habitat characteristics, identify key predictors of occupancy, and model how those patterns may shift under future climate scenarios. Climatic variables such as temperature extremes and precipitation levels will be tested against occupancy data to determine their influence on pygmy rabbit distribution. This could ultimately allow researchers to forecast how suitable habitats may expand, contract, or shift over the coming decades.<\/p>\n<p>Additionally, by comparing new survey data with results from a similar study conducted during 2009-2012 by the Idaho Department of Fish &amp; Game in southeast Idaho, the team hopes to detect long-term trends in occupancy in parts of the State. While early impressions suggest pygmy rabbit distribution may not have changed drastically, this comparison will offer one of the first rigorous evaluations of population trends over time.<\/p>\n<p>Meanwhile, the threat of Rabbit Hemorrhagic Disease Virus (RHDV2)\u2014a highly infectious and often fatal virus affecting wild and domestic rabbits\u2014adds urgency to the work. Documented in pygmy rabbits in Nevada, the disease represents a major risk and adds urgency to ongoing monitoring and habitat protection.<\/p>\n<h3 class=\"wp-block-heading\">The Bigger Picture: Resilience, Interconnection, and Hope<\/h3>\n<p>In total, this work is providing much-needed clarity about the pygmy rabbit\u2019s current status and future prospects. And while the project focuses on a single species, its broader impact ripples across the sagebrush steppe\u2014a diverse and increasingly vulnerable ecosystem that supports more than 350 species of vertebrates and plays a key role in Western livelihoods, recreation, and watershed health.<\/p>\n<div class=\"wp-block-media-text is-stacked-on-mobile\">\n<figure class=\"wp-block-media-text__media\"><img loading=\"lazy\" decoding=\"async\" width=\"960\" height=\"687\" src=\"https:\/\/depts.washington.edu\/pnwcesu\/wordpress\/wp-content\/uploads\/2025\/10\/Project-Highlight-Visuals-6.jpg\" alt=\"\" class=\"wp-image-18995 size-full\" srcset=\"https:\/\/depts.washington.edu\/pnwcesu\/wordpress\/wp-content\/uploads\/2025\/10\/Project-Highlight-Visuals-6.jpg 960w, https:\/\/depts.washington.edu\/pnwcesu\/wordpress\/wp-content\/uploads\/2025\/10\/Project-Highlight-Visuals-6-300x215.jpg 300w, https:\/\/depts.washington.edu\/pnwcesu\/wordpress\/wp-content\/uploads\/2025\/10\/Project-Highlight-Visuals-6-768x550.jpg 768w, https:\/\/depts.washington.edu\/pnwcesu\/wordpress\/wp-content\/uploads\/2025\/10\/Project-Highlight-Visuals-6-375x268.jpg 375w, https:\/\/depts.washington.edu\/pnwcesu\/wordpress\/wp-content\/uploads\/2025\/10\/Project-Highlight-Visuals-6-750x537.jpg 750w\" sizes=\"auto, (max-width: 960px) 100vw, 960px\" \/><\/figure>\n<div class=\"wp-block-media-text__content\">\n<p>If we want to conserve wildlife, it starts with habitat\u2014and understanding that these ecosystems are complex, dynamic, and deeply interconnected. Every organism, no matter how small, plays a role in maintaining the health of our landscapes. By mapping pygmy rabbit presence and protecting the habitats they depend on, researchers and land managers are doing far more than conserving a single species\u2014they\u2019re laying the groundwork for protecting an entire ecosystem.<\/p>\n<\/div>\n<\/div>\n<p>In many ways, the pygmy rabbit is an ideal symbol of resilience in the face of habitat fragmentation, disease, and climate change. Small but mighty, hard to find but essential, they remind us that unassuming creatures often carry great ecological weight.<\/p>\n<p>And as this extraordinary collaborative effort shows, protecting them requires a similarly determined and coordinated response. I can\u2019t think of a better mascot to champion resilience in the face of habitat and climatic changes, and I aspire to remain as dedicated as the mighty pygmy rabbits.<\/p>\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n<p><em>A special thank you to Dr. Janet Rachlow, Fiona McKibben, Dr. Leona Svancara with the Northwest Climate Adaptation Science Center, the University of Idaho, U.S. Fish and Wildlife Service, Bureau of Land Management, National Park Service, U.S. Forest Service, Idaho Fish &amp; Game, Idaho National Laboratory, the Montana Department of Fish, Wildlife &amp; Parks, and the many biologists who helped contribute to these impactful projects!<\/em><\/p>\n","protected":false},"excerpt":{"rendered":"<p>By Kylie Baker Mapping the Hidden World of Pygmy Rabbits: Genetic Tools and Collaborative Surveys in Idaho\u2019s Sagebrush Steppe In the arid expanses of the sagebrush steppe, an elusive burrower quietly reshapes the landscape in profound ways: the pygmy rabbit. As the smallest rabbit species in the world, pygmy rabbits (Brachylagus idahoensis) are sagebrush obligates,&#8230;<\/p>\n<div><a class=\"more\" href=\"https:\/\/depts.washington.edu\/pnwcesu\/project-highlight-pygmy-rabbits-in-idaho-using-genetic-analyses-to-estimate-occupancy\/\">Read more<\/a><\/div>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[3621],"tags":[3625],"class_list":["post-18987","post","type-post","status-publish","format-standard","hentry","category-project-highlights","tag-projecthighlight"],"acf":[],"publishpress_future_action":{"enabled":false,"date":"2026-04-19 11:46:23","action":"change-status","newStatus":"draft","terms":[],"taxonomy":"category","extraData":[]},"publishpress_future_workflow_manual_trigger":{"enabledWorkflows":[]},"_links":{"self":[{"href":"https:\/\/depts.washington.edu\/pnwcesu\/wp-json\/wp\/v2\/posts\/18987","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/depts.washington.edu\/pnwcesu\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/depts.washington.edu\/pnwcesu\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/pnwcesu\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/depts.washington.edu\/pnwcesu\/wp-json\/wp\/v2\/comments?post=18987"}],"version-history":[{"count":3,"href":"https:\/\/depts.washington.edu\/pnwcesu\/wp-json\/wp\/v2\/posts\/18987\/revisions"}],"predecessor-version":[{"id":19003,"href":"https:\/\/depts.washington.edu\/pnwcesu\/wp-json\/wp\/v2\/posts\/18987\/revisions\/19003"}],"wp:attachment":[{"href":"https:\/\/depts.washington.edu\/pnwcesu\/wp-json\/wp\/v2\/media?parent=18987"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/depts.washington.edu\/pnwcesu\/wp-json\/wp\/v2\/categories?post=18987"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/depts.washington.edu\/pnwcesu\/wp-json\/wp\/v2\/tags?post=18987"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}