South Lake Union Lab Services
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The Cell and Tissue Culture Cost Center at SLU is located in Brotman basement room B-56. A satellite facility is also operated at Health Sciences Building room E-507. Mr. Hoa Nguyen is the lead technician for this cost center. Marcus Biondi provides relief assistance. The office may be reached at (206) 685-2720.
Hours of operation are 7:00am to 6:00pm M-F. The doors to B-56 remain unlocked after hours to allow drop-off of carts with items to be processed and for access to the dry ice supply. Please note that no machines in B-56 are available for general use.
Services offered for fee include:
- Glass Wash
- Autoclave- clean autoclaving of solutions
- Pipettes- glass pipette washing and plugging
- Biowaste- autoclave processing of biowaste and dirty items to be sterilized
- Sharps- offsite disposal of sharps containers
- CO2- monitoring and supply to labs
A dry ice supply for general use is maintained in B-56, but billed separately from this cost center.
Glass Wash and Dry-Heat Sterilization
All labs are expected to have labeled the PI's 3 initials on all lab-ware, etching being the preferred method for glass items.
Pre-rinse lab-ware after use and pre-soak in a 10% bleach solution appropriate vessels needing de-contamination prior to being brought to B-56.
The preferred method to deliver items for processing in B-56 is on a cart also labeled with the PI's initials or name. When a cart is not practical for the amount being delivered please use a labeled pan or basin. There are a limited number of buckets available in B-56 for transport of lab-ware on a first come first served basis. If your lab has their own buckets, please permanently label to prevent them becoming a generally distributed item.
All lab-ware is washed in an ultra-clean cycle that includes a heated acid and RO water rinse.
Dry-heat sterilization is offered upon request. The dry-heat sterilizer is operated only during the evening hours when the room is unoccupied.
- It is preferred that stir-bars be cleaned in the lab and not in B-56.
- All surgical instruments are to be cleaned in the lab and not in B-56.
- All incubator parts brought for washing to B-56 must be labeled with the labs identifiers. If autoclaving is desired, parts should be placed in clear autoclaving bags (275F) for processing.
Autoclaving, of both liquid and dry items, is done on a first come, first served basis. Please note that for liquids that arrive in B-56 before 3:00pm, all reasonable efforts will be extended to insure that these liquids are autoclaved that day. Liquids arriving at 3:01 or later will be autoclaved as scheduling allows. In general, dry items for autoclave will be turned around in 24 hours; no special arrangements are offered for retrieval outside of our regular hours.
It is expected that all items to be autoclaved will be identified with the PI's 3 initials. If not brought down on a cart, please leave your items on the shelves immediately to your left as you enter B-56.
Processed items not on carts can be found on the shelves about 12 feet inside of B-56, also to the left.
Carts with processed items or empty biohazard bins can be found either inside B-56 or in the hallway behind the door marked B-15.
Washing and plugging service is currently provided for glass pipettes; turn around time may be up to 4 days.
Use of disposable pipettes is encouraged. Pipettes or pipette tips for disposal must be packaged in "keepers". Keepers are essentially cardstock boxes for safe handling of these items prior to disposal. These containers are closed and put into bio-hazardous waste bags as any other waste item. Keepers, when closed properly, prevent puncturing the biowaste bags, thus minimizing potential injury to personnel during autoclaving and disposal.
Dry, non-volatile, bio-hazardous waste, void of all specimens, human or animal, is autoclaved on site in B-56. Bio-hazardous waste is collected into bags, purchased by the lab, prominently stenciled with the biohazard symbol and able to withstand autoclaving at 275F. Any waste bags not certified to 275F will be returned for repackaging. Every bag of waste is to have the PI's name plus a minimum of a 3" length of autoclave tape adhered to the side. Biowaste bags are to be closed either with a commercially available clip or "bunched" at the top in a fashion that leaves an opening approximately 1 inch in diameter.
Each lab is encouraged to centralize their dry biohazard collection somewhere in their lab. Red biohazard bins are provided for this purpose. Labs are requested to label the bins with the PI's 3 letter initials and the room of origin. To be in compliance with EH&S, each re-usable biowaste collection container is to have a liner stenciled with the biohazard symbol. Again, please note that these liner bags are to be autoclaveable at 275F.
Use the provided red biowaste bins, complete with lid, to transport waste to B-56. The preferred way to do this is on a lab cart. In the event that B-56 is unattended, DO NOT leave biohazard waste bags on the floor. Expect to leave the lab cart, plus bin, and return 4 hours later for retrieval.
Bins are the unit of charge. Any bins delivered to B-56 that are less than 75% full will be charged estimated by percent of volume of the bin. Biowaste bags larger than 75% of the volume of a red bin are not accepted and will be returned to the lab of origin for re-packaging.
For safety, Agar plates are to be segregated, placed in a keeper (see pipettes, above) and bagged with the word AGAR prominently labeled plus the PI's name written on the outside.
NOTE: All biowaste is to be contained in biowaste bags able to withstand autoclaving at 275F. Every bag of waste is to have the PI's name plus a minimum of a 3" length of autoclave tape adhered to each bag.
Sharps (collected in hard plastic sharps containers) are brought to B-56 for off-site shipment and processing by a University contractor. Label all sharps containers with the PI's initials and room number of origin.
Broken Lab Glass
Non-biohazard broken lab glass is disposed of as described on the EH&S web site.
CO2 supplies drawn from piped wall turrets at SLU are managed from central closets on each research floor. The Cell and Tissue Culture team meets with researchers to define their need, in terms of number of items hooked into the system. A ratio, based on each lab's amount of equipment drawing from the system, is then used to calculate percentage of CO2 used. Monitoring by CTC staff ensures an uninterrupted supply.
Dry-ice pellets are also available in B-56 on a self-serve basis. Pre-tare your container on the balances provided, weigh what you need, and enter the data required on the sign-out sheet. Prior to taking dry ice, please be sure to provide the appropriate budget number the application is to be charged against. This service is billed separately from the Cell and Tissue Culture Cost Center. Dry ice is delivered Monday and Thursday mornings so please notify Hoa, Marcus or Matthew Curry if need for an unusually large amount is expected.
Biowaste Bin Liners (autoclavable to 275F)
Diversified Medical Products (800-825-5224); cat #9538E
Whitney Products (800-DEVICES);
Bench-Top Keepers BH2006 (3x3x15.5") (pipettes)
BH2003 (6x6x10") (pipettes, plates, tubes, etc)
BH2002 (4x6x7") (pipette tips, tubes, etc.)
BH2005 (5x2x11") (pipette tips, tubes, swabs, etc)
Other Autoclavable Bags
Inquire about size practical for your use and can withstand 275F.
Diversified Medical Products