Klavins Lab Turbidostat Wiki | OperatingManual / SetupAssembleClosedSystem

August 16, 2014, at 04:05 PM by chris -

Changed line 70 from:

Wrap all culture vessel export ports in aluminum foil

to:

Wrap all culture vessel effluent tube openings in aluminum foil

Changed line 72 from:

Double check that all open ends of the tubing system, export ports, and air filters are properly wrapped in aluminum foil

to:

Double check that all open ends of the tubing system, effluent ports, and air filters are properly wrapped in aluminum foil

Added line 80:

As soon as the autoclave finishes remove the foil from only the air filters. This will allow them to dry. Only dry filters will pass air.

August 16, 2014, at 04:02 PM by chris -

Changed lines 65-68 from:

Connect a Luer lock end of a 2' tubing section of the octopus tubing system to an inport port on the culture vessel
Connect an air filter to the other inport port on the culture vessel
Repeat for the 7 remaining culture vessels
- After this step, the culture vessels should only have their export ports open to the environment

to:

For each culture tube
1. Connect one of the Female Luer ends from the culture tube to one of the male ends on the tubing manifold.
2. Connect an air filter to the other Female Luer port on the culture vessel
- After this step, the culture vessels should only have their effluent ports open

August 16, 2014, at 03:57 PM by chris -

Changed lines 52-54 from:

Wash with soap and water
- Make sure to clean all biofilms off of culture tube walls
Rinse culture tube with ethanol

to:

Wash with soap and water using a tube brush

Changed lines 54-55 from:

Clean magnetic stir bar
1. Rinse with ethanol

to:

Clean magnetic stir bar with soap and water

Changed line 56 from:

Place stir bar into culture tube with a little bit of DI water

to:

Place stir bar into culture tube with a little bit of DI water (whatever is left from rinsing is okay)

Changed lines 58-61 from:

Plug

Rinse out input ports with ethanol
Rinse out export port with ethanol
Securely place plug into culture tube

to:

Stopper

Rinse out input ports with ethanol from a squeeze bottle
Rinse out export port with ethanol from a squeeze bottle
Securely place Stopper into culture tube

August 16, 2014, at 03:47 PM by chris -

Changed line 44 from:

Fasten Luer lock connectors to tubing created in step 1

to:

Fasten a Male Luer lock connector to tubing created in step 1

Changed line 46 from:

Attach Teflon air filter to connecter side of tubing

to:

Attach the female end of the Teflon air filter to other end of the tubing

August 16, 2014, at 03:43 PM by chris -

Changed lines 17-18 from:

(2) 3" tube

to:

(1) 1" tube
(1) 3" tube

Changed line 20 from:

(1) 5' long tube

to:

(1) 5' tube

Added lines 22-26:

Attach two 6-way barb manifolds end-to-end with the 1" section of tube.
On the two other ends attach the 3" and 5' tubes.
On the remaining 8 ends of the barb manifolds attach the 8 2' tubes.
On the 8 2' pieces of tubing attach male Luer-barb connectors.
on the 2 remaining pieces, connect female Luer-barb connectors.

Changed line 42 from:

Cut tubing (2cm diameter?)

to:

Cut tubing (1/8" ID x 1/4" OD)

August 16, 2014, at 03:36 PM by chris -

Changed line 16 from:

Cut Tubing (50mm diameter?)

to:

Cut Tubing (1/16" ID 1/8" OD silicone). Note: you may adjust the length of tubing as needed for your configuration.

August 16, 2014, at 03:34 PM by chris -

Changed line 15 from:

If preparing for the first time, do steps 1-2 else skip to 3

to:

If preparing for the first time.

Added lines 22-26:

If reusing tubing.

Inspect the tubing.
1. Check the tubing for holes and leaks.
2. Check the tubing for fatigue cause by the pinch valves. Look for dents in the tubing, if it looks bad enough or tears when stretched, replace the tubing.

Changed lines 30-32 from:

Rinse with DI water

to:

Rinse with DI water.
1. Note: It's important to thoroughly rinse all tubing. Any left over media will burn in the autoclave and may discolor the media used to blank the system.

August 16, 2014, at 03:29 PM by chris -

Changed line 20 from:

Assemble tubes together in an "octopus" system

to:

Assemble tubing manifold

Changed line 22 from:

Rinse octopus tubing system

to:

Rinse tubing system

Added line 24:

using a squeeze bottle flush ethanol into each opening of the 10 way tubing manifold.

August 16, 2014, at 03:27 PM by chris -

Changed line 10 from:

Six Way Connector\\\

to:

Six Way Manifold\\\

August 14, 2014, at 09:06 PM by chris -

Deleted line 36:

NEED TO EXPLAIN HOW TO CREATE STOPPER WITH NEEDLES IN IT

August 14, 2014, at 09:04 PM by chris -

Changed line 67 from:

Set the temperature to 121 deg C?

to:

Set the temperature to 121 °C?

August 03, 2012, at 05:58 PM by felixekn -

Added lines 6-7:

Male Luer Lock

Deleted lines 8-9:

Male Luer Lock\\\

July 13, 2012, at 11:50 PM by felixekn -

Added line 37:

NEED TO EXPLAIN HOW TO CREATE STOPPER WITH NEEDLES IN IT

July 13, 2012, at 11:36 PM by felixekn -

Changed line 18 from:

(4) 2' tube

to:

(8) 2' tube

May 01, 2012, at 05:01 PM by felixekn -

Changed line 1 from:

Next ?

to:

Next ?

to:

Changed line 1 from:

Next ?

Changed line 70 from:

Next ?

April 16, 2012, at 04:10 PM by felixekn -

Added line 1:

Continue to next section

to:

Changed lines 67-69 from:

Setting is a preset called "Packs" on some autoclaves

to:

Setting is a preset called "Packs" on some autoclaves

Continue to next section

April 16, 2012, at 05:14 AM by felixekn -

Changed line 6 from:

to:

Changed line 8 from:

to:

Changed lines 10-11 from:

https://students.washington.edu/cnt/pmwiki/pub/uploads/OperatingManual/SetupAssembleClosedSystem/AssembledTubing.jpeg Assembled Tubing

to:

https://students.washington.edu/cnt/pmwiki/pub/uploads/OperatingManual/SetupAssembleClosedSystem/AssembledTubing.jpeg Assembled Tubing

https://students.washington.edu/cnt/pmwiki/pub/uploads/OperatingManual/SetupAssembleClosedSystem/InputConnection.jpeg Input port connection

April 16, 2012, at 05:11 AM by felixekn -

Changed lines 10-11 from:

https://students.washington.edu/cnt/pmwiki/pub/uploads/OperatingManual/SetupAssembleClosedSystem/AssembledTubing.jpeg Assembled Tubing

to:

https://students.washington.edu/cnt/pmwiki/pub/uploads/OperatingManual/SetupAssembleClosedSystem/AssembledTubing.jpeg Assembled Tubing

April 16, 2012, at 02:32 AM by felixekn -

Changed line 4 from:

\\

to:

Changed line 6 from:

\\

to:

Changed line 8 from:

\\

to:

April 15, 2012, at 09:40 PM by felixekn -

Changed line 1 from:

NEED: Picture of air filters, diagram of input and export holes, and completed closed system

to:

NEED: Picture of air filters, diagram of input and export holes on plug, and completed closed system

April 15, 2012, at 09:40 PM by felixekn -

Added lines 1-2:

NEED: Picture of air filters, diagram of input and export holes, and completed closed system

April 15, 2012, at 09:38 PM by felixekn -

Changed line 16 from:

Assemble tubes together

to:

Assemble tubes together in an "octopus" system

Changed line 18 from:

Rinse tubing

to:

Rinse octopus tubing system

Changed line 50 from:

Connect a Luer lock end of a 2' tubing section to an inport port on the culture vessel

to:

Connect a Luer lock end of a 2' tubing section of the octopus tubing system to an inport port on the culture vessel

Changed line 61 from:

Place closed system into an autoclave with

to:

Place closed tubing-culture vessel system into an autoclave

Changed line 63 from:

Run autoclave for 30? minutes

to:

Run autoclave for 30 minutes

April 15, 2012, at 09:20 PM by felixekn -

Changed line 44 from:

Rinse out inport ports with ethanol

to:

Rinse out input ports with ethanol

Deleted lines 45-47:

Repeat steps 1-2 for the 7 remaning culture tubes

Assembly

Place stir bar into culture tube

April 15, 2012, at 09:18 PM by felixekn -

Changed lines 33-37 from:

Clean culture tube with water and soap
- Take care to clear all biofilms off of culture tube walls
  - It's okay for stir bar to be in or out of the culture vessel
- Rinse culture tube with ethanol
- Rinse culture tube with DI water

to:

Clean culture tube
1. Wash with soap and water
  - Make sure to clean all biofilms off of culture tube walls
2. Rinse culture tube with ethanol
3. Rinse culture tube with DI water

Changed lines 39-40 from:

Rinse with ethanol

to:

Rinse with ethanol
Rinse with DI water

April 15, 2012, at 09:15 PM by felixekn -

Changed line 34 from:

Take care to clear all biofilms off of culture vessel

to:

Take care to clear all biofilms off of culture tube walls

Changed lines 36-39 from:

Rinse culture tube with ethanol
Rinse culture tube with DI water
Rinse stir bar with ethanol
Place stir bar into culture tube

to:

Rinse culture tube with ethanol
Rinse culture tube with DI water

Clean magnetic stir bar
- Rinse with ethanol
Place stir bar into culture tube with a little bit of DI water

April 15, 2012, at 09:13 PM by felixekn -

Added line 29:

See Images to the left:

April 15, 2012, at 09:11 PM by felixekn -

Changed line 16 from:

Fasten connectors to tubing

to:

Assemble tubes together

April 14, 2012, at 09:23 PM by felixekn -

Changed lines 1-2 from:

\\

to:

Parts
\\

Added line 9:

April 12, 2012, at 09:49 PM by felixekn -

Changed line 7 from:

Assembled Tubing

to:

https://students.washington.edu/cnt/pmwiki/pub/uploads/OperatingManual/SetupAssembleClosedSystem/AssembledTubing.jpeg Assembled Tubing

April 12, 2012, at 09:45 PM by felixekn -

Changed line 1 from:

\\

to:

\\

April 12, 2012, at 09:44 PM by felixekn -

Changed line 2 from:

Female Luer Lock\\\

to:

Female Luer Lock\\\

Changed line 4 from:

Male Luer Lock\\\

to:

Male Luer Lock\\\

Changed lines 6-7 from:

Six Way Connector

Assembled Tubing

to:

Six Way Connector

Assembled Tubing

April 12, 2012, at 09:44 PM by felixekn -

Added lines 1-7:

Female Luer Lock

Male Luer Lock

Six Way Connector

Assembled Tubing

Changed lines 15-20 from:

See images bellow:

Female Luer Lock

Male Luer Lock

Six Way Connector

Assembled Tubing

to:

See images to the left:

Changed lines 25-28 from:

See Images Bellow

Female Luer Lock

to:

See Images to the left:

April 12, 2012, at 09:25 PM by felixekn -

Changed lines 10-14 from:

Female Luer Lock

Male Luer Lock

Six Way Connector

Assembled Tubing

\\

to:

Female Luer Lock

Male Luer Lock

Six Way Connector

Assembled Tubing

April 12, 2012, at 09:18 PM by felixekn -

Changed lines 10-13 from:

Female Luer Lock

Male Luer Lock

Six Way Connector

Assembled Tubing

to:

Female Luer Lock

Male Luer Lock

Six Way Connector

Assembled Tubing

April 12, 2012, at 09:17 PM by felixekn -

Changed line 13 from:

Assembled Tubing

to:

Assembled Tubing

April 12, 2012, at 09:16 PM by felixekn -

Changed line 12 from:

Attach:5WayConnector.jpeg Δ | Five Way Connector

to:

Six Way Connector

Changed line 14 from:

to:

\\

Changed line 18 from:

to:

\\

April 12, 2012, at 09:15 PM by felixekn -

April 12, 2012, at 08:59 PM by felixekn -

Changed line 11 from:

Attach:LuerLockMale1.jpeg Δ | Male Luer Lock

to:

Male Luer Lock

April 12, 2012, at 08:59 PM by felixekn -

Changed line 13 from:

Assembled Tubing

to:

Assembled Tubing

April 12, 2012, at 08:58 PM by felixekn -

Changed lines 8-14 from:

See diagram bellow (not implemented yet)

to:

See images bellow:

Female Luer Lock

Attach:LuerLockMale1.jpeg Δ | Male Luer Lock Attach:5WayConnector.jpeg Δ | Five Way Connector

Assembled Tubing

Changed lines 23-25 from:

Fasten Luer lock connector to tubing created in step 1
- See diagram bellow (not implemented yet)

to:

Fasten Luer lock connectors to tubing created in step 1
- See Images Bellow

Female Luer Lock

Changed lines 64-67 from:

Setting is a preset called "Packs" on some autoclaves

http://pmichaud.com/img/misc/pc.jpg | [- [[ Wikipedia:Paper_clips | Paper clips ]] are fun to work with. -]

to:

Setting is a preset called "Packs" on some autoclaves

April 12, 2012, at 08:05 PM by cnt -

Changed line 19 from:

""

to:

Changed line 59 from:

Wikipedia:Paper_clips | Paper clips ]] are fun to work with. -]

to:

Wikipedia:Paper_clips | Paper clips ]] are fun to work with. -]

April 12, 2012, at 07:59 PM by felixekn -

Changed lines 56-59 from:

Setting is a preset called "Packs" on some autoclaves

to:

Setting is a preset called "Packs" on some autoclaves

http://pmichaud.com/img/misc/pc.jpg | [- [[ Wikipedia:Paper_clips | Paper clips ]] are fun to work with. -]

April 12, 2012, at 07:50 PM by felixekn -

Changed line 19 from:

"Attach:LuerLockFem1.JPG Δ"

to:

""

April 12, 2012, at 07:18 PM by felixekn -

Changed line 19 from:

"Attach:foo.jpg Δ"

to:

"Attach:LuerLockFem1.JPG Δ"

April 12, 2012, at 07:04 PM by felixekn -

Added line 19:

"Attach:foo.jpg Δ"

April 10, 2012, at 08:56 PM by felixekn -

Changed lines 53-54 from:

Set the temperature to 125� C?

Run autoclave for 30? minutes

to:

Set the temperature to 121� C?

Run autoclave for 30? minutes
- Setting is a preset called "Packs" on some autoclaves

April 10, 2012, at 03:55 AM by felixekn -

April 10, 2012, at 02:41 AM by felixekn -

Changed lines 52-54 from:

Place closed system into an autoclave with a set temperature of 125� C? for 30? minutes

to:

Place closed system into an autoclave with
- Set the temperature to 125� C?
Run autoclave for 30? minutes

April 10, 2012, at 02:40 AM by felixekn -

Changed lines 49-52 from:

NOTE: All aluminum foil wraps should be tight enough so that no liquid is able to enter the now enclosed tubing-culture vessel system

to:

NOTE: All aluminum foil wraps should be tight enough so that no liquid is able to enter the now enclosed tubing-culture vessel system

AUTOCLAVE CLOSED SYSTEM

Place closed system into an autoclave with a set temperature of 125� C? for 30? minutes

April 10, 2012, at 02:33 AM by felixekn -

April 10, 2012, at 02:31 AM by felixekn -

Changed lines 15-19 from:

to:

Cut tubing (2cm diameter?)
- (8) 6" tube
Fasten Luer lock connector to tubing created in step 1
- See diagram bellow (not implemented yet)
Attach Teflon air filter to connecter side of tubing

April 10, 2012, at 02:29 AM by felixekn -

Changed lines 1-2 from:

TUBING PREPARATION

If preparing for the first time, do steps 1-2 else skip to 3

to:

TUBING PREPARATION

If preparing for the first time, do steps 1-2 else skip to 3

Changed lines 13-14 from:

CULTURE VESSEL PREPARATION

Body

to:

AIR FILTER PREPARATION

Execute steps bellow only if no air filters are created or need replacing

CULTURE VESSEL PREPARATION

Body

Changed line 27 from:

Plug

to:

Plug

Changed line 31 from:

Assembly

to:

Assembly

Changed line 36 from:

CLOSED SYSTEM ASSEMBLY

to:

CLOSED SYSTEM ASSEMBLY

April 10, 2012, at 02:26 AM by felixekn -

April 10, 2012, at 02:25 AM by felixekn -

Changed lines 14-15 from:

Apply to all culture vessels

Body

to:

Body

Changed lines 22-23 from:

Plug

to:

Repeat steps 1-5 for the 7 remaning culture tubes

Plug

Changed lines 26-27 from:

Assembly

to:

Repeat steps 1-2 for the 7 remaning culture tubes

Assembly

Changed lines 30-31 from:

to:

Repeat steps 1-2 for the 7 remaning culture tubes

Changed line 41 from:

NOTE: All aluminum foil wraps should be tight enough so that no liquid is able to enter the now enclosed tubing-culture vessel system

to:

NOTE: All aluminum foil wraps should be tight enough so that no liquid is able to enter the now enclosed tubing-culture vessel system

April 10, 2012, at 02:23 AM by felixekn -

Changed line 16 from:

Clean culture vessel with water and soap

to:

Clean culture tube with water and soap

Changed lines 19-20 from:

Rinse culture vessel with ethanol
Rinse culture vessel with DI water

to:

Rinse culture tube with ethanol
Rinse culture tube with DI water

Changed line 22 from:

Place stir bar into culture vessel

to:

Place stir bar into culture tube

Changed lines 27-29 from:

Place stir bar into culture vessel
Securely place plug into culture vessel

to:

Place stir bar into culture tube
Securely place plug into culture tube

Changed lines 31-39 from:

Connect Luer lock ends of the 2' tubing sections to an inport port on the culture vessel

to:

Connect a Luer lock end of a 2' tubing section to an inport port on the culture vessel
Connect an air filter to the other inport port on the culture vessel
Repeat for the 7 remaining culture vessels
- After this step, the culture vessels should only have their export ports open to the environment
Wrap all open ends of the tubing system in aluminum foil
Wrap all culture vessel export ports in aluminum foil
Wrap the air filter in aluminum foil
- Double check that all open ends of the tubing system, export ports, and air filters are properly wrapped in aluminum foil

NOTE: All aluminum foil wraps should be tight enough so that no liquid is able to enter the now enclosed tubing-culture vessel system

April 10, 2012, at 02:14 AM by felixekn -

Changed line 15 from:

BODY

to:

Body

Changed lines 23-24 from:

'PLUG

Rinse out input ports with ethanol

to:

Plug

Rinse out inport ports with ethanol

Changed line 26 from:

ASSEMBLY

to:

Assembly

Added line 31:

Connect Luer lock ends of the 2' tubing sections to an inport port on the culture vessel

April 10, 2012, at 02:12 AM by felixekn -

Changed lines 15-16 from:

Rinse culture vessel with water and soap

to:

BODY

Clean culture vessel with water and soap

Added lines 19-30:

Rinse culture vessel with ethanol
Rinse culture vessel with DI water
Rinse stir bar with ethanol
Place stir bar into culture vessel

'PLUG

Rinse out input ports with ethanol
Rinse out export port with ethanol

ASSEMBLY

Place stir bar into culture vessel
Securely place plug into culture vessel

CLOSED SYSTEM ASSEMBLY

April 10, 2012, at 02:07 AM by felixekn -

Changed lines 15-17 from:

to:

Rinse culture vessel with water and soap
- Take care to clear all biofilms off of culture vessel
  - It's okay for stir bar to be in or out of the culture vessel

April 10, 2012, at 02:02 AM by felixekn -

Deleted lines 0-8:

MATERIALS

50mm? diameter tubing
2cm? diameter tubing
(16) ?gage syringe
(8) Foam stopper
(8) Glass capillary
(8) Teflon air filter

Added line 2:

If preparing for the first time, do steps 1-2 else skip to 3

April 10, 2012, at 02:00 AM by felixekn -

Added lines 1-23:

MATERIALS

50mm? diameter tubing
2cm? diameter tubing
(16) ?gage syringe
(8) Foam stopper
(8) Glass capillary
(8) Teflon air filter

TUBING PREPARATION

Cut Tubing (50mm diameter?)
- (2) 3" tube
- (4) 2' tube
- (1) 5' long tube
Fasten connectors to tubing
- See diagram bellow (not implemented yet)
Rinse tubing
1. Rinse with ethanol
2. Rinse with DI water

CULTURE VESSEL PREPARATION

Apply to all culture vessels

SetupAssembleClosedSystem

OperatingManual.SetupAssembleClosedSystem History

NEED: Picture of air filters, diagram of input and export holes, and completed closed system

NEED: Picture of air filters, diagram of input and export holes on plug, and completed closed system

NEED: Picture of air filters, diagram of input and export holes, and completed closed system