|
Information
Operating
Manual
Construction
Manual
Derivative
Designs
Downloads
PmWiki
edit SideBar
|
OperatingManual.SetupAttachMachinery History
Show minor edits - Show changes to markup
Changed lines 1-2 from:
to:
Changed lines 54-55 from:
to:
Added line 1:
Changed line 53 from:
to:
Added lines 1-2:
NEED: Diagrams for attaching the syringe to the pump, diagram for placing culture vessels in their chambers, diagrams for connecting effluent tubes to waste containers
Deleted lines 48-50:
FINAL STEPS
- Unclamp the media line originating from the media filled carboy
Deleted line 1:
All steps bellow are performed inside of an incubator
Changed lines 14-16 from:
to:
- Tape the top of each culture vessel to its chamber
- Turn on chambers (switch is outside of incubator)
Added lines 49-52:
FINAL STEPS
- Unclamp the media line originating from the media filled carboy
Changed lines 30-34 from:
- Unwrap aluminum foil from a Teflon air filter
- Connect previously unwrapped Teflon air filter to tubing hanging from incubator ceiling
- Repeat for the remaining 7 Teflon air filters
- Turn on air pumps (switch is outside of incubator)
to:
- Connect air intakes to air pumps
- Unwrap aluminum foil from a Teflon air filter
- Connect previously unwrapped Teflon air filter to tubing hanging from incubator ceiling
- Repeat substeps 1-2 for the remaining 7 Teflon air filters
- Turn on air pumps (switch is outside of incubator)
Changed lines 24-26 from:
- Feed the 2' sections of the octopus lines through the valves around the motor. One at a time, remove the plastic covering piece by sliding it down and off.
- Insert the line and slide the plastic piece back on from the bottom.
- Culture vessel in chamber 1 should be connected to pinch valve 1 (furthest pinch valve from opening of incubator) and the rest should follow in that order
to:
- Feed the 2' sections of the octopus lines through the valves around the motor. One at a time, remove the plastic covering piece by sliding it down and off.
- Insert the line and slide the plastic piece back on from the bottom.
- Culture vessel in chamber 1 should be connected to pinch valve 1 (furthest pinch valve from opening of incubator) and the rest should follow in that order
Changed line 37 from:
- Spray ethanol onto specially made waste container connector???? (has 4 holes for tubing)
to:
- Spray ethanol onto effluent tube holder (each holder takes 4 effluent tubes)
Changed lines 39-40 from:
- Place effluent tubing from the culture vessel in the first most chamber into the waste container connector???
- Unwrap aluminum foil from effluent tubing
to:
- Attach effluent tubes to effluent tube holder in order (culture vessel 1 then 2 then 3 etc.)
- Unwrap aluminum foil from effluent tube
Changed lines 42-43 from:
- Insert tubing into the waste container connector
to:
- Insert tube into the effluent tube holder
- Repeat substeps steps 1-3 for remaining culture vessels
Changed lines 45-46 from:
- IN ORDER Repeat steps 1-2 for the next 3 culture vessels
- Insert waste container connector??? into a waste bottle
to:
- Insert effluent tube holder into a waste bottles
Deleted line 46:
- Repeat steps 1-4 for the remaining 4 culture vessels
Changed line 18 from:
Tubing inserted into pinch valve\\\
to:
Figure 1: Tubing inserted into pinch valve\\\
Changed lines 20-21 from:
Air filters connected to culture vessels
to:
Figure 2:Air filters connected to culture vessels
Changed lines 27-28 from:
to:
Changed line 32 from:
to:
Changed line 16 from:
to:
Changed line 16 from:
to:
Changed line 20 from:
to:
Air filters connected to culture vessels
Changed lines 16-21 from:
Tubing inserted into pinch valveto:
Parts
Tubing inserted into pinch valve
Air filters connected
Deleted line 28:
Added line 2:
All steps bellow are performed inside of an incubator
Changed line 13 from:
- Place each culture vessel into a chamber in order of how they are connected to the tubing system
to:
- Inside the incubator place each culture vessel into a chamber in order of how they are connected to the octopus tubing system
Changed line 23 from:
CONNECT AIR TUBING TO PRESSURE REGULATORS
to:
CONNECT AIR INTAKE TUBING TO AIR PUMPS
Added line 28:
- Turn on air pumps (switch is outside of incubator)
Added lines 18-19:
- Feed the 2' sections of the octopus lines through the valves around the motor. One at a time, remove the plastic covering piece by sliding it down and off.
- Insert the line and slide the plastic piece back on from the bottom.
Changed line 21 from:
to:
Added line 15:
Tubing inserted into pinch valveDeleted line 19:
Tubing inserted into pinch valve
Changed line 19 from:
to:
Tubing inserted into pinch valve
Changed line 19 from:
to:
Changed line 6 from:
- Fasten bracing bracket around the newly inserted 5 ml syringe
to:
- Fasten bracing bracket around the newly inserted syringe
Changed line 8 from:
- Place tubing connected to the 5 ml syringe between the solenoid pinch valve
to:
- Place tubing connected to the syringe between the solenoid pinch valve
Added line 19:
Changed line 4 from:
- Place 5 ml syringe into holder
to:
- Place syringe connected to 3' tube of octopus tubing system into holder
Changed line 15 from:
ATTACH MEDIA TUBES TO PINCH VALVES
to:
ATTACH MEDIA TUBING TO PINCH VALVES
Changed line 26 from:
CONNECT EFFLUENT TUBES TO WASTE BOTTLES
to:
CONNECT EFFLUENT TUBING TO WASTE BOTTLES
Changed lines 36-37 from:
- Repeat steps 1-4 for the remaining 4 culture vessels
to:
- Repeat steps 1-4 for the remaining 4 culture vessels
Changed line 35 from:
- Insert waste container connector??? into one of the two waste bottles
to:
- Insert waste container connector??? into a waste bottle
Changed lines 16-17 from:
- Place the media input tubing that connects to the input ports on the culture vessels into their respective punch valve
- Culture vessel in chamber 1 should be connected to punch valve 1 (furthest pinch valve from opening of incubator)
to:
- Place the media input tubing that connects to the input ports on the culture vessels into their respective pinch valve
- Culture vessel in chamber 1 should be connected to pinch valve 1 (furthest pinch valve from opening of incubator) and the rest should follow in that order
Changed line 29 from:
- Place effluent tubing from the culture vessel in chamber 1 into the waste container connector???
to:
- Place effluent tubing from the culture vessel in the first most chamber into the waste container connector???
Changed lines 34-36 from:
- IN ORDER Repeat steps 1-2 for the remaining 7 culture vessels
to:
- IN ORDER Repeat steps 1-2 for the next 3 culture vessels
- Insert waste container connector??? into one of the two waste bottles
- Repeat steps 1-4 for the remaining 4 culture vessels
Changed lines 30-33 from:
- Unwrap aluminum foil from effluent tubing
- Spray newly exposed end with ethanol
- Insert tubing into the waste container connector
- IN ORDER Repeat steps 1-2 for the remaining 7 culture vessels
to:
- Unwrap aluminum foil from effluent tubing
- Spray newly exposed end with ethanol
- Insert tubing into the waste container connector
- IN ORDER Repeat steps 1-2 for the remaining 7 culture vessels
Changed lines 32-33 from:
to:
- Insert tubing into the waste container connector
- IN ORDER Repeat steps 1-2 for the remaining 7 culture vessels
Changed lines 29-32 from:
- Unwrap aluminum foil from effluent tubing of culture vessel in chamber 1
to:
- Place effluent tubing from the culture vessel in chamber 1 into the waste container connector???
- Unwrap aluminum foil from effluent tubing
- Spray newly exposed end with ethanol
-
Changed lines 11-12 from:
PLACE CULTURE VESSELS INTO THEIR HOLDERS
- Place each culture vessel into a holder in order of how they are connected to the tubing system
to:
PLACE CULTURE VESSELS INTO THEIR CHAMBERS
- Place each culture vessel into a chamber in order of how they are connected to the tubing system
Added lines 27-29:
- Spray ethanol onto specially made waste container connector???? (has 4 holes for tubing)
- Unwrap aluminum foil from effluent tubing of culture vessel in chamber 1
Changed lines 21-23 from:
CONNECT EFFLUENT TUBES TO WASTE BOTTLES\\
to:
- Unwrap aluminum foil from a Teflon air filter
- Connect previously unwrapped Teflon air filter to tubing hanging from incubator ceiling
- Repeat for the remaining 7 Teflon air filters
CONNECT EFFLUENT TUBES TO WASTE BOTTLES
Changed line 1 from:
ATTACH SYRINGE TO SYRINGE PUMP\\
to:
ATTACH SYRINGE TO SYRINGE PUMP
Changed line 11 from:
PLACE CULTURE VESSELS INTO THEIR HOLDERS\\
to:
PLACE CULTURE VESSELS INTO THEIR HOLDERS
Changed line 15 from:
ATTACH MEDIA TUBES TO PINCH VALVES\\
to:
ATTACH MEDIA TUBES TO PINCH VALVES
Changed line 20 from:
CONNECT AIR TUBING TO PRESSURE REGULATORS\\
to:
CONNECT AIR TUBING TO PRESSURE REGULATORS
Deleted lines 10-11:
ATTACH MEDIA TUBES TO PINCH VALVES
Changed lines 12-19 from:
to:
- Place each culture vessel into a holder in order of how they are connected to the tubing system
ATTACH MEDIA TUBES TO PINCH VALVES
#Place the media input tubing that connects to the input ports on the culture vessels into their respective punch valve
- Culture vessel in chamber 1 should be connected to punch valve 1 (furthest pinch valve from opening of incubator)
- (diagram coming soon)
Added line 21:
Changed lines 2-10 from:
to:
- Unscrew syringe pump bracing bracket
- Place 5 ml syringe into holder
- Fasten bracing bracket around the newly inserted 5 ml syringe
- Place tubing connected to the 5 ml syringe between the solenoid pinch valve
Changed line 17 from:
CONNECT EFFLUENT TUBES TO WASTE BOTTLES\\
to:
CONNECT EFFLUENT TUBES TO WASTE BOTTLES\\
Added line 2:
Added line 4:
Added line 6:
Added line 8:
Added lines 1-5:
ATTACH SYRINGE TO SYRINGE PUMP]
[ATTACH MEDIA TUBES TO PINCH VALVES]
[PLACE CULTURE VESSELS INTO THEIR HOLDERS]
[CONNECT AIR TUBING TO PRESSURE REGULATORS]
[CONNECT EFFLUENT TUBES TO WASTE BOTTLES\\
|
