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Information Operating
Derivative |
SetupAttachMachineryOperatingManual.SetupAttachMachinery HistoryHide minor edits - Show changes to output Changed line 2 from:
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[[OperatingManual/SetupPrimePump | Next]] Changed lines 1-2 from:
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[[OperatingManual/SetupAttachOpenEnds | Previous]] [[OperatingManualSetupPrimePump | Next]] Changed lines 54-55 from:
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[[OperatingManual/SetupAttachOpenEnds | Previous]] [[OperatingManual/SetupPrimePump | Next]] Added line 1:
[[https://students.washington.edu/cnt/pmwiki/pmwiki.php?n=OperatingManual.SetupAttachOpenEnds | Previous]] [[https://students.washington.edu/cnt/pmwiki/pmwiki.php?n=OperatingManual.SetupPrimePump | Next]] Changed line 53 from:
[[https://students.washington.edu/cnt/pmwiki/pmwiki.php?n=OperatingManual. to:
[[https://students.washington.edu/cnt/pmwiki/pmwiki.php?n=OperatingManual.SetupAttachOpenEnds | Previous]] [[https://students.washington.edu/cnt/pmwiki/pmwiki.php?n=OperatingManual.SetupPrimePump | Next]] Added line 52:
[[https://students.washington.edu/cnt/pmwiki/pmwiki.php?n=OperatingManual.SetupPrimePump | Continue to next section]] Added lines 1-2:
!!!NEED: Diagrams for attaching the syringe to the pump, diagram for placing culture vessels in their chambers, diagrams for connecting effluent tubes to waste containers Deleted lines 48-50:
[+'''@@FINAL STEPS@@'''+] #Unclamp the media line originating from the media filled carboy Deleted line 1:
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#Tape the top of each culture vessel to its chamber #Turn on chambers (switch is outside of incubator) Added lines 49-52:
[+'''@@FINAL STEPS@@'''+] #Unclamp the media line originating from the media filled carboy Changed lines 30-34 from:
# #Connect previously unwrapped **See Figure 2 #Repeat for the remaining 7 Teflon air filters #Turn to:
#Connect air intakes to air pumps ##Unwrap aluminum foil from a Teflon air filter ##Connect previously unwrapped Teflon air filter to tubing hanging from incubator ceiling ***See Figure 2 ##Repeat substeps 1-2 for the remaining 7 Teflon air filters ##Turn on air pumps (switch is outside of incubator) Changed lines 24-26 from:
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##Feed the 2' sections of the octopus lines through the valves around the motor. One at a time, remove the plastic covering piece by sliding it down and off. ##Insert the line and slide the plastic piece back on from the bottom. ##Culture vessel in chamber 1 should be connected to pinch valve 1 (furthest pinch valve from opening of incubator) and the rest should follow in that order Changed line 37 from:
#Spray ethanol onto to:
#Spray ethanol onto effluent tube holder (each holder takes 4 effluent tubes) Changed lines 39-40 from:
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#Attach effluent tubes to effluent tube holder in order (culture vessel 1 then 2 then 3 etc.) ##Unwrap aluminum foil from effluent tube Changed lines 42-43 from:
##Insert to:
##Insert tube into the effluent tube holder ##Repeat substeps steps 1-3 for remaining culture vessels Changed lines 45-46 from:
# #Insert waste container connector??? into a waste bottle to:
#Insert effluent tube holder into a waste bottles Deleted line 46:
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[-Tubing inserted into pinch valve-]\\\ to:
[-'''''Figure 1:''''' Tubing inserted into pinch valve-]\\\ Changed lines 20-21 from:
[-Air filters connected to culture vessels-] to:
[-'''''Figure 2:'''''Air filters connected to culture vessels-] Changed lines 27-28 from:
***See to:
***See Figure 1 Changed line 32 from:
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**See Figure 2 Changed line 16 from:
%center%%block rframe width=190px% [+{+'''Setup'''+}+]\\ to:
%center%%block rframe width=190px% [+{+'''Setup Diagrams'''+}+]\\ Changed line 16 from:
%center%%block rframe width=190px% [ to:
%center%%block rframe width=190px% [+{+'''Setup'''+}+]\\ Changed line 20 from:
[-Air filters connected-] to:
[-Air filters connected to culture vessels-] Changed lines 16-21 from:
%center%% to:
%center%%block rframe width=190px% [-Parts-]\\ %width=190px%Attach:PinchValve.jpeg [-Tubing inserted into pinch valve-]\\\ %width=190px%Attach:AirFilterConnection.jpeg [-Air filters connected-] Deleted line 28:
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'''All steps bellow are performed inside of an incubator''' Changed line 13 from:
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#Inside the incubator place each culture vessel into a chamber in order of how they are connected to the octopus tubing system Changed line 23 from:
[+'''@@CONNECT AIR TUBING TO to:
[+'''@@CONNECT AIR INTAKE TUBING TO AIR PUMPS@@'''+] Added line 28:
#Turn on air pumps (switch is outside of incubator) Added lines 18-19:
**Feed the 2' sections of the octopus lines through the valves around the motor. One at a time, remove the plastic covering piece by sliding it down and off. **Insert the line and slide the plastic piece back on from the bottom. Changed line 21 from:
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***See image to the left Added line 15:
%center%%boxed rframe%Attach:PinchValve.jpeg | [-Tubing inserted into pinch valve-] Deleted line 19:
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Attach:PinchValve. to:
%center%%boxed rframe%Attach:PinchValve.jpeg | [-Tubing inserted into pinch valve-] Changed line 19 from:
Attach: to:
Attach:PinchValve.jpeg Changed line 6 from:
#Fasten bracing bracket around the newly inserted to:
#Fasten bracing bracket around the newly inserted syringe Changed line 8 from:
#Place tubing connected to the to:
#Place tubing connected to the syringe between the solenoid pinch valve Added line 19:
Attach: PinchValve.jpeg Changed line 4 from:
#Place to:
#Place syringe connected to 3' tube of octopus tubing system into holder Changed line 15 from:
[+'''@@ATTACH MEDIA to:
[+'''@@ATTACH MEDIA TUBING TO PINCH VALVES@@'''+] Changed line 26 from:
[+'''@@CONNECT EFFLUENT to:
[+'''@@CONNECT EFFLUENT TUBING TO WASTE BOTTLES@@'''+] Changed lines 36-37 from:
#Repeat steps 1-4 for the remaining 4 culture vessels to:
**(diagram coming soon) #Repeat steps 1-4 for the remaining 4 culture vessels Changed line 35 from:
#Insert waste container connector??? into to:
#Insert waste container connector??? into a waste bottle Changed lines 16-17 from:
#Place the media input tubing that connects to the input ports on the culture vessels into their respective **Culture vessel in chamber 1 should be connected to to:
#Place the media input tubing that connects to the input ports on the culture vessels into their respective pinch valve **Culture vessel in chamber 1 should be connected to pinch valve 1 (furthest pinch valve from opening of incubator) and the rest should follow in that order Changed line 29 from:
#Place effluent tubing from the culture vessel in to:
#Place effluent tubing from the culture vessel in the first most chamber into the waste container connector??? Changed lines 34-36 from:
#'''IN ORDER''' Repeat steps 1-2 for the to:
#'''IN ORDER''' Repeat steps 1-2 for the next 3 culture vessels #Insert waste container connector??? into one of the two waste bottles #Repeat steps 1-4 for the remaining 4 culture vessels Changed lines 30-33 from:
#'''IN ORDER''' Repeat steps 1-2 for the remaining 7 culture vessels to:
##Unwrap aluminum foil from effluent tubing ##Spray newly exposed end with ethanol ##Insert tubing into the waste container connector **(diagram coming soon) #'''IN ORDER''' Repeat steps 1-2 for the remaining 7 culture vessels Changed lines 32-33 from:
** to:
**Insert tubing into the waste container connector #'''IN ORDER''' Repeat steps 1-2 for the remaining 7 culture vessels Changed lines 29-32 from:
# to:
#Place effluent tubing from the culture vessel in chamber 1 into the waste container connector??? **Unwrap aluminum foil from effluent tubing **Spray newly exposed end with ethanol ** Changed lines 11-12 from:
[+'''@@PLACE CULTURE VESSELS INTO THEIR #Place each culture vessel into a to:
[+'''@@PLACE CULTURE VESSELS INTO THEIR CHAMBERS@@'''+] #Place each culture vessel into a chamber in order of how they are connected to the tubing system Added lines 27-29:
#Spray ethanol onto specially made waste container connector???? (has 4 holes for tubing) **(diagram coming soon) #Unwrap aluminum foil from effluent tubing of culture vessel in chamber 1 Changed lines 21-23 from:
[+'''@@CONNECT EFFLUENT TUBES TO WASTE BOTTLES@@ to:
#Unwrap aluminum foil from a Teflon air filter #Connect previously unwrapped Teflon air filter to tubing hanging from incubator ceiling **(diagram coming soon) #Repeat for the remaining 7 Teflon air filters [+'''@@CONNECT EFFLUENT TUBES TO WASTE BOTTLES@@'''+] Changed line 1 from:
[+'''@@ATTACH SYRINGE TO SYRINGE PUMP@@'''+] to:
[+'''@@ATTACH SYRINGE TO SYRINGE PUMP@@'''+] Changed line 11 from:
[+'''@@PLACE CULTURE VESSELS INTO THEIR HOLDERS@@'''+] to:
[+'''@@PLACE CULTURE VESSELS INTO THEIR HOLDERS@@'''+] Changed line 15 from:
[+'''@@ATTACH MEDIA TUBES TO PINCH VALVES@@'''+] to:
[+'''@@ATTACH MEDIA TUBES TO PINCH VALVES@@'''+] Changed line 20 from:
[+'''@@CONNECT AIR TUBING TO PRESSURE REGULATORS@@'''+] to:
[+'''@@CONNECT AIR TUBING TO PRESSURE REGULATORS@@'''+] Deleted lines 10-11:
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#Place each culture vessel into a holder in order of how they are connected to the tubing system **(diagram coming soon) [+'''@@ATTACH MEDIA TUBES TO PINCH VALVES@@'''+]\\ #Place the media input tubing that connects to the input ports on the culture vessels into their respective punch valve **Culture vessel in chamber 1 should be connected to punch valve 1 (furthest pinch valve from opening of incubator) **(diagram coming soon) Added line 21:
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#Unscrew syringe pump bracing bracket **(diagram coming soon) #Place 5 ml syringe into holder **(diagram coming soon) #Fasten bracing bracket around the newly inserted 5 ml syringe **(diagram coming soon) #Place tubing connected to the 5 ml syringe between the solenoid pinch valve **(diagram coming soon) Changed line 17 from:
[+'''@@CONNECT EFFLUENT TUBES TO WASTE BOTTLES@@'''+]\\ to:
[+'''@@CONNECT EFFLUENT TUBES TO WASTE BOTTLES@@'''+]\\ Added lines 1-5:
[+'''@@ATTACH SYRINGE TO SYRINGE PUMP@@'''+]\\ [+'''@@ATTACH MEDIA TUBES TO PINCH VALVES@@'''+]\\ [+'''@@PLACE CULTURE VESSELS INTO THEIR HOLDERS@@'''+]\\ [+'''@@CONNECT AIR TUBING TO PRESSURE REGULATORS@@'''+]\\ [+'''@@CONNECT EFFLUENT TUBES TO WASTE BOTTLES@@'''+]\\ |