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OperatingManual.SetupAttachOpenEnds History
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Added line 5:
Note: It's best to spray your gloved hands in ethanol before preforming these steps.
Changed lines 7-10 from:
PERFORM BELLOW STEPS QUICKLY
- Take the free male Luer lock connector on the 3" tube of the octopus tubing system and unwrap the aluminum foil from it
- Attach a steril syringe to the unwrapped male Luer lock connector
to:
PERFORM BELLOW STEPS USING STERILE TECHNIQUE
- Take the free female Luer lock connector on the 3" end of the tubing manifold and unwrap the aluminum foil from it
- Attach a sterile syringe to the end you just unwrapped
Changed line 12 from:
PERFORM BELLOW STEPS QUICKLY
to:
PERFORM BELLOW STEPS USING STERILE TECHNIQUE
Deleted line 15:
- Spray ethanol onto both unwrapped connectors
Changed line 1 from:
to:
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to:
Added line 1:
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Changed line 15 from:
Continue to next section: Attach closed system to machinery
to:
Changed line 15 from:
to:
Continue to next section: Attach closed system to machinery
Changed line 15 from:
[[https://students.washington.edu/cnt/pmwiki/pmwiki.php?n=OperatingManual.SetupAttachMachinery | Continue to next section}}
to:
Added lines 14-15:
[[https://students.washington.edu/cnt/pmwiki/pmwiki.php?n=OperatingManual.SetupAttachMachinery | Continue to next section}}
Added lines 1-2:
NEED: Pictures of attaching syringe and media line to octopus tubing
Deleted lines 4-5:
PLACING INPUT TUBING INTO PINCH VALVES
Changed lines 3-4 from:
- Take free Luer lock connector on the 3" tube and unwrap the aluminum foil from it
- Unwrap a 5 ml?? syringe from its packaging and connect it to the unwrapped Luer lock connector
to:
- Take the free male Luer lock connector on the 3" tube of the octopus tubing system and unwrap the aluminum foil from it
- Attach a steril syringe to the unwrapped male Luer lock connector
PLACING INPUT TUBING INTO PINCH VALVES
Changed line 8 from:
- Take free Luer lock connector on the 4' tube and unwrap the aluminum foil from it
to:
- Take free Luer lock connector on the 4' tube (media line) and unwrap the aluminum foil from it
Changed line 10 from:
- Ethanol both unwrapped ends
to:
- Spray ethanol onto both unwrapped connectors
Changed lines 1-2 from:
ATTACH STERILE SYRINGE
[-PERFORM BELLOW STEPS QUICKLY]-
to:
ATTACH STERILE SYRINGE
PERFORM BELLOW STEPS QUICKLY
Changed lines 4-6 from:
- 'Unwrap a 5 ml?? syringe from its packaging and connect it to the unwrapped Luer lock connector
ATTACH MEDIA LINE TO MEDIA BOTTLE
to:
- Unwrap a 5 ml?? syringe from its packaging and connect it to the unwrapped Luer lock connector
ATTACH MEDIA LINE TO MEDIA BOTTLE\\
Changed lines 2-4 from:
- Take free Luer lock connector on the 3' tube and unwrap the aluminum foil from it
- QUICKLY unwrap a 5 ml?? syringe from its packaging and connect it to the unwrapped Luer lock connector
to:
[-PERFORM BELLOW STEPS QUICKLY]-
- Take free Luer lock connector on the 3" tube and unwrap the aluminum foil from it
- 'Unwrap a 5 ml?? syringe from its packaging and connect it to the unwrapped Luer lock connector
ATTACH MEDIA LINE TO MEDIA BOTTLE
PERFORM BELLOW STEPS QUICKLY
- Take free Luer lock connector on the 4' tube and unwrap the aluminum foil from it
- Unrwap the aluminum foil from the output port on the media bottle
- Ethanol both unwrapped ends
- Connect the Luer lock of the media line to the output port on the media bottle
Added lines 1-4:
ATTACH STERILE SYRINGE
- Take free Luer lock connector on the 3' tube and unwrap the aluminum foil from it
- QUICKLY unwrap a 5 ml?? syringe from its packaging and connect it to the unwrapped Luer lock connector
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